Chromosome aberrations in pressure-induced triploid Atlantic salmon
Background Triploid organisms have three sets of chromosomes. In Atlantic salmon, hydrostatic pressure treatment of newly fertilized eggs has been extensively used to produce triploids which are functionally sterile due to their unpaired chromosomes. These fish often perform poorly on commercial far...
Published in: | BMC Genetics |
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Language: | English |
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2020
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Online Access: | http://hdl.handle.net/1893/31337 https://doi.org/10.1186/s12863-020-00864-0 http://dspace.stir.ac.uk/bitstream/1893/31337/1/s12863-020-00864-0.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/31337 |
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openpolar |
institution |
Open Polar |
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University of Stirling: Stirling Digital Research Repository |
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ftunivstirling |
language |
English |
topic |
Triploid Trisomy Aneuploid PUD Mosaic Aquaculture Environmental impact Welfare |
spellingShingle |
Triploid Trisomy Aneuploid PUD Mosaic Aquaculture Environmental impact Welfare Glover, Kevin A Harvey, Alison C Hansen, Tom Johnny Fjelldal, Per Gunnar Besnier, Francois N Bos, Jim Boy Ayllon, Fernando Taggart, John B Solberg, Monica F Chromosome aberrations in pressure-induced triploid Atlantic salmon |
topic_facet |
Triploid Trisomy Aneuploid PUD Mosaic Aquaculture Environmental impact Welfare |
description |
Background Triploid organisms have three sets of chromosomes. In Atlantic salmon, hydrostatic pressure treatment of newly fertilized eggs has been extensively used to produce triploids which are functionally sterile due to their unpaired chromosomes. These fish often perform poorly on commercial farms, sometimes without explanation. Inheritance patterns in individuals subjected to pressure treatment have not been investigated in Atlantic salmon thus far. However, work on other species suggests that this treatment can result in aberrant inheritance. We therefore studied this in Atlantic salmon by genotyping 16 polymorphic microsatellites in eyed eggs and juveniles which had been subjected to pressure-induction of triploidy. Communally reared juveniles including fish subjected to pressure-induction of triploidy and their diploid siblings were included as a control. Results No diploid offspring were detected in any of the eggs or juveniles which were subjected to hydrostatic pressure; therefore, the induction of triploidy was highly successful. Aberrant inheritance was nevertheless observed in 0.9% of the eggs and 0.9% of the juveniles that had been subjected to pressure treatment. In the communally reared fish, 0.3% of the fish subjected to pressure treatment displayed aberrant inheritance, while their diploid controls displayed 0% aberrant inheritance. Inheritance errors included two eyed eggs lacking maternal DNA across all microsatellites, and, examples in both eggs and juveniles of either the maternal or paternal allele lacking in one of the microsatellites. All individuals displaying chromosome aberrations were otherwise triploid. Conclusions This is the first study to document aberrant inheritance in Atlantic salmon that have been subjected to pressure-induction of triploidy. Our experiments unequivocally demonstrate that even when induction of triploidy is highly successful, this treatment can cause chromosome aberrations in this species. Based upon our novel data, and earlier studies in other organisms, we ... |
author2 |
Norwegian Institute of Marine Research ZEBCARE Institute of Aquaculture orcid:0000-0002-3843-9663 |
format |
Article in Journal/Newspaper |
author |
Glover, Kevin A Harvey, Alison C Hansen, Tom Johnny Fjelldal, Per Gunnar Besnier, Francois N Bos, Jim Boy Ayllon, Fernando Taggart, John B Solberg, Monica F |
author_facet |
Glover, Kevin A Harvey, Alison C Hansen, Tom Johnny Fjelldal, Per Gunnar Besnier, Francois N Bos, Jim Boy Ayllon, Fernando Taggart, John B Solberg, Monica F |
author_sort |
Glover, Kevin A |
title |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_short |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_full |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_fullStr |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_full_unstemmed |
Chromosome aberrations in pressure-induced triploid Atlantic salmon |
title_sort |
chromosome aberrations in pressure-induced triploid atlantic salmon |
publisher |
BMC |
publishDate |
2020 |
url |
http://hdl.handle.net/1893/31337 https://doi.org/10.1186/s12863-020-00864-0 http://dspace.stir.ac.uk/bitstream/1893/31337/1/s12863-020-00864-0.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Glover KA, Harvey AC, Hansen TJ, Fjelldal PG, Besnier FN, Bos JB, Ayllon F, Taggart JB & Solberg MF (2020) Chromosome aberrations in pressure-induced triploid Atlantic salmon. BMC Genetics, 21, Art. No.: 59. https://doi.org/10.1186/s12863-020-00864-0 59 http://hdl.handle.net/1893/31337 doi:10.1186/s12863-020-00864-0 WOS:000540458400001 1638916 http://dspace.stir.ac.uk/bitstream/1893/31337/1/s12863-020-00864-0.pdf |
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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. http://creativecommons.org/licenses/by/4.0/ |
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CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s12863-020-00864-0 |
container_title |
BMC Genetics |
container_volume |
21 |
container_issue |
1 |
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1766360411349712896 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/31337 2023-05-15T15:29:57+02:00 Chromosome aberrations in pressure-induced triploid Atlantic salmon Glover, Kevin A Harvey, Alison C Hansen, Tom Johnny Fjelldal, Per Gunnar Besnier, Francois N Bos, Jim Boy Ayllon, Fernando Taggart, John B Solberg, Monica F Norwegian Institute of Marine Research ZEBCARE Institute of Aquaculture orcid:0000-0002-3843-9663 2020 application/pdf http://hdl.handle.net/1893/31337 https://doi.org/10.1186/s12863-020-00864-0 http://dspace.stir.ac.uk/bitstream/1893/31337/1/s12863-020-00864-0.pdf en eng BMC Glover KA, Harvey AC, Hansen TJ, Fjelldal PG, Besnier FN, Bos JB, Ayllon F, Taggart JB & Solberg MF (2020) Chromosome aberrations in pressure-induced triploid Atlantic salmon. BMC Genetics, 21, Art. No.: 59. https://doi.org/10.1186/s12863-020-00864-0 59 http://hdl.handle.net/1893/31337 doi:10.1186/s12863-020-00864-0 WOS:000540458400001 1638916 http://dspace.stir.ac.uk/bitstream/1893/31337/1/s12863-020-00864-0.pdf This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. http://creativecommons.org/licenses/by/4.0/ CC0 PDM CC-BY Triploid Trisomy Aneuploid PUD Mosaic Aquaculture Environmental impact Welfare Journal Article VoR - Version of Record 2020 ftunivstirling https://doi.org/10.1186/s12863-020-00864-0 2022-06-13T18:45:14Z Background Triploid organisms have three sets of chromosomes. In Atlantic salmon, hydrostatic pressure treatment of newly fertilized eggs has been extensively used to produce triploids which are functionally sterile due to their unpaired chromosomes. These fish often perform poorly on commercial farms, sometimes without explanation. Inheritance patterns in individuals subjected to pressure treatment have not been investigated in Atlantic salmon thus far. However, work on other species suggests that this treatment can result in aberrant inheritance. We therefore studied this in Atlantic salmon by genotyping 16 polymorphic microsatellites in eyed eggs and juveniles which had been subjected to pressure-induction of triploidy. Communally reared juveniles including fish subjected to pressure-induction of triploidy and their diploid siblings were included as a control. Results No diploid offspring were detected in any of the eggs or juveniles which were subjected to hydrostatic pressure; therefore, the induction of triploidy was highly successful. Aberrant inheritance was nevertheless observed in 0.9% of the eggs and 0.9% of the juveniles that had been subjected to pressure treatment. In the communally reared fish, 0.3% of the fish subjected to pressure treatment displayed aberrant inheritance, while their diploid controls displayed 0% aberrant inheritance. Inheritance errors included two eyed eggs lacking maternal DNA across all microsatellites, and, examples in both eggs and juveniles of either the maternal or paternal allele lacking in one of the microsatellites. All individuals displaying chromosome aberrations were otherwise triploid. Conclusions This is the first study to document aberrant inheritance in Atlantic salmon that have been subjected to pressure-induction of triploidy. Our experiments unequivocally demonstrate that even when induction of triploidy is highly successful, this treatment can cause chromosome aberrations in this species. Based upon our novel data, and earlier studies in other organisms, we ... Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository BMC Genetics 21 1 |