Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection
Amoebic gill disease (AGD) is emerging as one of the most significant health challenges affecting farmed Atlantic salmon in the marine environment. It is caused by the amphizoic amoeba Neoparamoeba perurans, with infestation of gills causing severe hyperplastic lesions, compromising overall gill int...
Published in: | Journal of Fish Diseases |
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Main Authors: | , , , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Wiley
2019
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Subjects: | |
Online Access: | http://hdl.handle.net/1893/30287 https://doi.org/10.1111/jfd.13073 http://dspace.stir.ac.uk/bitstream/1893/30287/1/no%20corrections%20version%2023%2007%20sf%20ammended%20SFChang%20et%20al%202019.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/30287 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
ionoregulatory cells mitochondria‐rich cell Na+/K+‐ATPase Neoparamoeba perurans salmonid transmission electron microscopy |
spellingShingle |
ionoregulatory cells mitochondria‐rich cell Na+/K+‐ATPase Neoparamoeba perurans salmonid transmission electron microscopy Chang, Yao-Chung Hamlin-Wright, Harry Monaghan, Sean Herath, Tharangani Baily, Johanna del Pozo, Jorge Downes, Jamie Preston, Andrew Chalmers, Lynn Jayasuriya, Nilantha Bron, James E Adams, Alexandra Fridman, Sophie Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection |
topic_facet |
ionoregulatory cells mitochondria‐rich cell Na+/K+‐ATPase Neoparamoeba perurans salmonid transmission electron microscopy |
description |
Amoebic gill disease (AGD) is emerging as one of the most significant health challenges affecting farmed Atlantic salmon in the marine environment. It is caused by the amphizoic amoeba Neoparamoeba perurans, with infestation of gills causing severe hyperplastic lesions, compromising overall gill integrity and function. This study used histology, transmission electron microscopy (TEM), immunohistochemistry and transcript expression to relate AGD‐associated pathological changes to changes in the morphology and distribution of chloride cells (CCs) in the gills of Atlantic salmon (Salmo salar L.) showing the progression of an AGD infection. A marked reduction in numbers of immunolabelled CCs was detected, and a changing pattern in distribution and morphology was closely linked with the level of basal epithelial hyperplasia in the gill. In addition, acute degenerative ultrastructural changes to CCs at the lesion site were observed with TEM. These findings were supported by the early‐onset downregulation of Na+/K+‐ATPase transcript expression. This study provides supportive evidence that histological AGD lesion assessment was a good qualitative tool for AGD scoring and corresponded well with qPCR genomic Paramoeba perurans quantification. Ultrastructural changes induced in salmon CCs as a result of AGD are reported here for the first time. |
author2 |
BBSRC Biotechnology and Biological Sciences Research Council European Commission Institute of Aquaculture Harper Adams University University of Edinburgh Marine Institute (Ireland) University of Bristol orcid:0000-0002-7692-7756 orcid:0000-0002-2242-7078 orcid:0000-0002-3587-5160 orcid:0000-0002-1271-2991 orcid:0000-0003-3544-0519 orcid:0000-0002-0159-0474 |
format |
Article in Journal/Newspaper |
author |
Chang, Yao-Chung Hamlin-Wright, Harry Monaghan, Sean Herath, Tharangani Baily, Johanna del Pozo, Jorge Downes, Jamie Preston, Andrew Chalmers, Lynn Jayasuriya, Nilantha Bron, James E Adams, Alexandra Fridman, Sophie |
author_facet |
Chang, Yao-Chung Hamlin-Wright, Harry Monaghan, Sean Herath, Tharangani Baily, Johanna del Pozo, Jorge Downes, Jamie Preston, Andrew Chalmers, Lynn Jayasuriya, Nilantha Bron, James E Adams, Alexandra Fridman, Sophie |
author_sort |
Chang, Yao-Chung |
title |
Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection |
title_short |
Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection |
title_full |
Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection |
title_fullStr |
Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection |
title_full_unstemmed |
Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection |
title_sort |
changes in distribution, morphology and ultrastructure of chloride cell in atlantic salmon during an agd infection |
publisher |
Wiley |
publishDate |
2019 |
url |
http://hdl.handle.net/1893/30287 https://doi.org/10.1111/jfd.13073 http://dspace.stir.ac.uk/bitstream/1893/30287/1/no%20corrections%20version%2023%2007%20sf%20ammended%20SFChang%20et%20al%202019.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Chang Y, Hamlin-Wright H, Monaghan S, Herath T, Baily J, del Pozo J, Downes J, Preston A, Chalmers L, Jayasuriya N, Bron JE, Adams A & Fridman S (2019) Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection. Journal of Fish Diseases, 42 (10), pp. 1433-1446. https://doi.org/10.1111/jfd.13073 Development of autogenous vaccines for the control of amoebic gill disease in farmed Atlantic salmon ParaFishControl - Advanced Tools and Research Strategies for Parasite Control in European farmed fish BB/M028224/1 Grant Agreement no 634429 http://hdl.handle.net/1893/30287 doi:10.1111/jfd.13073 31429104 WOS:000481926900001 2-s2.0-85070888640 1442393 http://dspace.stir.ac.uk/bitstream/1893/30287/1/no%20corrections%20version%2023%2007%20sf%20ammended%20SFChang%20et%20al%202019.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Chang, Y‐C, Hamlin‐Wright, H, Monaghan, S, et al. Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection. J Fish Dis. 2019; 42: 1433– 1446, which has been published in final form at https://doi.org/10.1111/jfd.13073. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving. 2020-08-21 [no corrections version 23 07 sf ammended SFChang et al 2019.pdf] Publisher requires embargo of 12 months after formal publication. |
op_doi |
https://doi.org/10.1111/jfd.13073 |
container_title |
Journal of Fish Diseases |
container_volume |
42 |
container_issue |
10 |
container_start_page |
1433 |
op_container_end_page |
1446 |
_version_ |
1766361049163890688 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/30287 2023-05-15T15:30:36+02:00 Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection Chang, Yao-Chung Hamlin-Wright, Harry Monaghan, Sean Herath, Tharangani Baily, Johanna del Pozo, Jorge Downes, Jamie Preston, Andrew Chalmers, Lynn Jayasuriya, Nilantha Bron, James E Adams, Alexandra Fridman, Sophie BBSRC Biotechnology and Biological Sciences Research Council European Commission Institute of Aquaculture Harper Adams University University of Edinburgh Marine Institute (Ireland) University of Bristol orcid:0000-0002-7692-7756 orcid:0000-0002-2242-7078 orcid:0000-0002-3587-5160 orcid:0000-0002-1271-2991 orcid:0000-0003-3544-0519 orcid:0000-0002-0159-0474 2019-10 application/pdf http://hdl.handle.net/1893/30287 https://doi.org/10.1111/jfd.13073 http://dspace.stir.ac.uk/bitstream/1893/30287/1/no%20corrections%20version%2023%2007%20sf%20ammended%20SFChang%20et%20al%202019.pdf en eng Wiley Chang Y, Hamlin-Wright H, Monaghan S, Herath T, Baily J, del Pozo J, Downes J, Preston A, Chalmers L, Jayasuriya N, Bron JE, Adams A & Fridman S (2019) Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection. Journal of Fish Diseases, 42 (10), pp. 1433-1446. https://doi.org/10.1111/jfd.13073 Development of autogenous vaccines for the control of amoebic gill disease in farmed Atlantic salmon ParaFishControl - Advanced Tools and Research Strategies for Parasite Control in European farmed fish BB/M028224/1 Grant Agreement no 634429 http://hdl.handle.net/1893/30287 doi:10.1111/jfd.13073 31429104 WOS:000481926900001 2-s2.0-85070888640 1442393 http://dspace.stir.ac.uk/bitstream/1893/30287/1/no%20corrections%20version%2023%2007%20sf%20ammended%20SFChang%20et%20al%202019.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Chang, Y‐C, Hamlin‐Wright, H, Monaghan, S, et al. Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection. J Fish Dis. 2019; 42: 1433– 1446, which has been published in final form at https://doi.org/10.1111/jfd.13073. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving. 2020-08-21 [no corrections version 23 07 sf ammended SFChang et al 2019.pdf] Publisher requires embargo of 12 months after formal publication. ionoregulatory cells mitochondria‐rich cell Na+/K+‐ATPase Neoparamoeba perurans salmonid transmission electron microscopy Journal Article AM - Accepted Manuscript 2019 ftunivstirling https://doi.org/10.1111/jfd.13073 2022-06-13T18:44:27Z Amoebic gill disease (AGD) is emerging as one of the most significant health challenges affecting farmed Atlantic salmon in the marine environment. It is caused by the amphizoic amoeba Neoparamoeba perurans, with infestation of gills causing severe hyperplastic lesions, compromising overall gill integrity and function. This study used histology, transmission electron microscopy (TEM), immunohistochemistry and transcript expression to relate AGD‐associated pathological changes to changes in the morphology and distribution of chloride cells (CCs) in the gills of Atlantic salmon (Salmo salar L.) showing the progression of an AGD infection. A marked reduction in numbers of immunolabelled CCs was detected, and a changing pattern in distribution and morphology was closely linked with the level of basal epithelial hyperplasia in the gill. In addition, acute degenerative ultrastructural changes to CCs at the lesion site were observed with TEM. These findings were supported by the early‐onset downregulation of Na+/K+‐ATPase transcript expression. This study provides supportive evidence that histological AGD lesion assessment was a good qualitative tool for AGD scoring and corresponded well with qPCR genomic Paramoeba perurans quantification. Ultrastructural changes induced in salmon CCs as a result of AGD are reported here for the first time. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Journal of Fish Diseases 42 10 1433 1446 |