Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)
Supplies of marine fish oils (FO ) are limited, and sustainable production in aquaculture dictates that alternatives, such as vegetable oils, must be found that do not compromise fish health and product quality. Nutrigenomics is anticipated to provide an increased understanding of how nutrition infl...
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American Society for Nutritional Sciences
2005
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ftunivstirling:oai:dspace.stir.ac.uk:1893/2917 2023-05-15T15:30:46+02:00 Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) Jordal, Ann-Elise O Torstensen, Bente E Tsoi, Stephen Tocher, Douglas R Lall, Santosh P Douglas, Susan E National Institute of Nutrition and Seafood Research (NIFES) Institute of Aquaculture orcid:0000-0002-8603-9410 2005-10 application/pdf http://hdl.handle.net/1893/2917 http://jn.nutrition.org/content/135/10/2355.abstract http://dspace.stir.ac.uk/bitstream/1893/2917/1/Jordal%20final.pdf en eng American Society for Nutritional Sciences Jordal AO, Torstensen BE, Tsoi S, Tocher DR, Lall SP & Douglas SE (2005) Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). Journal of Nutrition, 135 (10), pp. 2355-2361. http://jn.nutrition.org/content/135/10/2355.abstract http://hdl.handle.net/1893/2917 http://jn.nutrition.org/content/135/10/2355.abstract 2-s2.0-26444552886 836979 http://dspace.stir.ac.uk/bitstream/1893/2917/1/Jordal%20final.pdf The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 3000-01-01 [Jordal final.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. Atlantic salmon Salmo salar fish ol vegetable oil gene expression microarray QPCR Lipid metabolism Fishes Feeding and feeds Fishes Nutrition Dietary supplements Fishes Quality Journal Article AM - Accepted Manuscript 2005 ftunivstirling 2022-06-13T18:43:24Z Supplies of marine fish oils (FO ) are limited, and sustainable production in aquaculture dictates that alternatives, such as vegetable oils, must be found that do not compromise fish health and product quality. Nutrigenomics is anticipated to provide an increased understanding of how nutrition influences metabolic pathways and homeostatic control, and may be used to measure and validate subtle changes in organ-specific, metabolic gene expression signatures. We have compared two groups of Atlantic salmon fed diets containing 100% FO or 75% rapeseed oil (RO)/25% FO for 42 weeks. A small-scale cDNA microarray was constructed to screen for changes in expression of lipid metabolism genes in the liver resulting from this partial substitution of RO for FO. Fatty acid desaturase gene expression was significantly increased in fish fed 75% RO compared to fish fed the control diet; this was confirmed by quantitative Real Time PCR analysis (Q-PCR). In addition, several genes, among these mitochondrial proteins, peroxisome proliferator activated receptor-γ (PPARγ) as well as other transcription factors, co-activators and signal transducers, showed significant differential regulation. This partially validated microarray may be used for further gene expression profiling using other dietary comparisons. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon Salmo salar fish ol vegetable oil gene expression microarray QPCR Lipid metabolism Fishes Feeding and feeds Fishes Nutrition Dietary supplements Fishes Quality |
spellingShingle |
Atlantic salmon Salmo salar fish ol vegetable oil gene expression microarray QPCR Lipid metabolism Fishes Feeding and feeds Fishes Nutrition Dietary supplements Fishes Quality Jordal, Ann-Elise O Torstensen, Bente E Tsoi, Stephen Tocher, Douglas R Lall, Santosh P Douglas, Susan E Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
topic_facet |
Atlantic salmon Salmo salar fish ol vegetable oil gene expression microarray QPCR Lipid metabolism Fishes Feeding and feeds Fishes Nutrition Dietary supplements Fishes Quality |
description |
Supplies of marine fish oils (FO ) are limited, and sustainable production in aquaculture dictates that alternatives, such as vegetable oils, must be found that do not compromise fish health and product quality. Nutrigenomics is anticipated to provide an increased understanding of how nutrition influences metabolic pathways and homeostatic control, and may be used to measure and validate subtle changes in organ-specific, metabolic gene expression signatures. We have compared two groups of Atlantic salmon fed diets containing 100% FO or 75% rapeseed oil (RO)/25% FO for 42 weeks. A small-scale cDNA microarray was constructed to screen for changes in expression of lipid metabolism genes in the liver resulting from this partial substitution of RO for FO. Fatty acid desaturase gene expression was significantly increased in fish fed 75% RO compared to fish fed the control diet; this was confirmed by quantitative Real Time PCR analysis (Q-PCR). In addition, several genes, among these mitochondrial proteins, peroxisome proliferator activated receptor-γ (PPARγ) as well as other transcription factors, co-activators and signal transducers, showed significant differential regulation. This partially validated microarray may be used for further gene expression profiling using other dietary comparisons. |
author2 |
National Institute of Nutrition and Seafood Research (NIFES) Institute of Aquaculture orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Jordal, Ann-Elise O Torstensen, Bente E Tsoi, Stephen Tocher, Douglas R Lall, Santosh P Douglas, Susan E |
author_facet |
Jordal, Ann-Elise O Torstensen, Bente E Tsoi, Stephen Tocher, Douglas R Lall, Santosh P Douglas, Susan E |
author_sort |
Jordal, Ann-Elise O |
title |
Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_short |
Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_full |
Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_fullStr |
Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_sort |
dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in atlantic salmon (salmo salar l.) |
publisher |
American Society for Nutritional Sciences |
publishDate |
2005 |
url |
http://hdl.handle.net/1893/2917 http://jn.nutrition.org/content/135/10/2355.abstract http://dspace.stir.ac.uk/bitstream/1893/2917/1/Jordal%20final.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Jordal AO, Torstensen BE, Tsoi S, Tocher DR, Lall SP & Douglas SE (2005) Dietary rapeseed oil affects the expression of genes involved in hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). Journal of Nutrition, 135 (10), pp. 2355-2361. http://jn.nutrition.org/content/135/10/2355.abstract http://hdl.handle.net/1893/2917 http://jn.nutrition.org/content/135/10/2355.abstract 2-s2.0-26444552886 836979 http://dspace.stir.ac.uk/bitstream/1893/2917/1/Jordal%20final.pdf |
op_rights |
The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 3000-01-01 [Jordal final.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. |
_version_ |
1766361214523277312 |