Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)
Background Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic salmon farming. Pyrethroids, which block arthropod voltage‐gated sodium channels (Nav1), are used for salmon delousing. However, pyrethroid resistance is common in L. salmonis. The present study charact...
Published in: | Pest Management Science |
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Main Authors: | , , , , , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Wiley
2019
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Online Access: | http://hdl.handle.net/1893/27612 https://doi.org/10.1002/ps.5151 http://dspace.stir.ac.uk/bitstream/1893/27612/1/Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf |
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openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Agronomy and Crop Science Insect Science General Medicine |
spellingShingle |
Agronomy and Crop Science Insect Science General Medicine Carmona-Antoñanzas, Greta Helgesen, Kari O Humble, Joseph L Tschesche, Claudia Bakke, Marit J Gamble, Louise Bekaert, Michaël Bassett, David I Horsberg, Tor E Bron, James E Sturm, Armin Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) |
topic_facet |
Agronomy and Crop Science Insect Science General Medicine |
description |
Background Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic salmon farming. Pyrethroids, which block arthropod voltage‐gated sodium channels (Nav1), are used for salmon delousing. However, pyrethroid resistance is common in L. salmonis. The present study characterised Nav1 homologues in L. salmonis in order to identify channel mutations associated to resistance, called kdr (knockdown) mutations. Results Genome scans identified three L. salmonis Nav1 homologues, LsNav1.1, LsNav1.2 and LsNav1.3. Arthropod kdr mutations map to specific Nav1 regions within domains DI‐III, namely segments S5 and S6 and the linker helix connecting S4 and S5. The above channel regions were amplified by RT‐PCR and sequenced in deltamethrin‐susceptible and deltamethrin‐resistant L. salmonis. While LsNav1.1 and LsNav1.2 lacked nucleotide polymorphisms showing association to resistance, LsNav1.3 showed a non‐synonymous mutation in S5 of DII occurring in deltamethrin‐resistant parasites. The mutation is homologous to a previously described kdr mutation (I936V, numbering according to Musca domestica Vssc1) and was present in two pyrethroid‐resistant L. salmonis strains (allele frequencies of 0.800 and 0.357), but absent in two pyrethroid‐susceptible strains. Conclusions The present study indicates that a kdr‐mutation in LsNaV 1.3 may contribute to deltamethrin resistance in L. salmonis. |
author2 |
Biotechnology and Biological Sciences Research Council Institute of Aquaculture Norwegian Veterinary Institute Norwegian University of Life Sciences orcid:0000-0002-1206-7654 orcid:0000-0002-7529-0829 orcid:0000-0003-3544-0519 orcid:0000-0003-2632-1999 |
format |
Article in Journal/Newspaper |
author |
Carmona-Antoñanzas, Greta Helgesen, Kari O Humble, Joseph L Tschesche, Claudia Bakke, Marit J Gamble, Louise Bekaert, Michaël Bassett, David I Horsberg, Tor E Bron, James E Sturm, Armin |
author_facet |
Carmona-Antoñanzas, Greta Helgesen, Kari O Humble, Joseph L Tschesche, Claudia Bakke, Marit J Gamble, Louise Bekaert, Michaël Bassett, David I Horsberg, Tor E Bron, James E Sturm, Armin |
author_sort |
Carmona-Antoñanzas, Greta |
title |
Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) |
title_short |
Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) |
title_full |
Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) |
title_fullStr |
Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) |
title_full_unstemmed |
Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) |
title_sort |
mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (lepeophtheirus salmonis) |
publisher |
Wiley |
publishDate |
2019 |
url |
http://hdl.handle.net/1893/27612 https://doi.org/10.1002/ps.5151 http://dspace.stir.ac.uk/bitstream/1893/27612/1/Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Carmona-Antoñanzas G, Helgesen KO, Humble JL, Tschesche C, Bakke MJ, Gamble L, Bekaert M, Bassett DI, Horsberg TE, Bron JE & Sturm A (2019) Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75 (2), pp. 527-536. https://doi.org/10.1002/ps.5151 Identifying molecular determinants of drug susceptibility in salmon lice (Lepeophtheirus salmonis) BB/L022923/1 http://hdl.handle.net/1893/27612 doi:10.1002/ps.5151 30062864 WOS:000455406600001 2-s2.0-85053841945 970527 http://dspace.stir.ac.uk/bitstream/1893/27612/1/Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Carmona‐Antoñanzas, G. , Helgesen, K. O., Humble, J. L., Tschesche, C. , Bakke, M. J., Gamble, L. , Bekaert, M. , Bassett, D. I., Horsberg, T. E., Bron, J. E. and Sturm, A. (2019), Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75: 527-536, which has been published in final form at https://doi.org/10.1002/ps.5151. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving. 2019-08-01 [Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf] Publisher requires embargo of 12 months after formal publication. |
op_doi |
https://doi.org/10.1002/ps.5151 |
container_title |
Pest Management Science |
container_volume |
75 |
container_issue |
2 |
container_start_page |
527 |
op_container_end_page |
536 |
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1766363015830044672 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/27612 2023-05-15T15:32:31+02:00 Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis) Carmona-Antoñanzas, Greta Helgesen, Kari O Humble, Joseph L Tschesche, Claudia Bakke, Marit J Gamble, Louise Bekaert, Michaël Bassett, David I Horsberg, Tor E Bron, James E Sturm, Armin Biotechnology and Biological Sciences Research Council Institute of Aquaculture Norwegian Veterinary Institute Norwegian University of Life Sciences orcid:0000-0002-1206-7654 orcid:0000-0002-7529-0829 orcid:0000-0003-3544-0519 orcid:0000-0003-2632-1999 2019-02-01 application/pdf http://hdl.handle.net/1893/27612 https://doi.org/10.1002/ps.5151 http://dspace.stir.ac.uk/bitstream/1893/27612/1/Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf en eng Wiley Carmona-Antoñanzas G, Helgesen KO, Humble JL, Tschesche C, Bakke MJ, Gamble L, Bekaert M, Bassett DI, Horsberg TE, Bron JE & Sturm A (2019) Mutations in voltage-gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75 (2), pp. 527-536. https://doi.org/10.1002/ps.5151 Identifying molecular determinants of drug susceptibility in salmon lice (Lepeophtheirus salmonis) BB/L022923/1 http://hdl.handle.net/1893/27612 doi:10.1002/ps.5151 30062864 WOS:000455406600001 2-s2.0-85053841945 970527 http://dspace.stir.ac.uk/bitstream/1893/27612/1/Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Carmona‐Antoñanzas, G. , Helgesen, K. O., Humble, J. L., Tschesche, C. , Bakke, M. J., Gamble, L. , Bekaert, M. , Bassett, D. I., Horsberg, T. E., Bron, J. E. and Sturm, A. (2019), Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis). Pest Management Science, 75: 527-536, which has been published in final form at https://doi.org/10.1002/ps.5151. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving. 2019-08-01 [Carmona-Anto-anzas_et_al-2018-Pest_Management_Science.pdf] Publisher requires embargo of 12 months after formal publication. Agronomy and Crop Science Insect Science General Medicine Journal Article AM - Accepted Manuscript 2019 ftunivstirling https://doi.org/10.1002/ps.5151 2022-06-13T18:42:11Z Background Parasitic salmon lice (Lepeophtheirus salmonis) cause high economic losses in Atlantic salmon farming. Pyrethroids, which block arthropod voltage‐gated sodium channels (Nav1), are used for salmon delousing. However, pyrethroid resistance is common in L. salmonis. The present study characterised Nav1 homologues in L. salmonis in order to identify channel mutations associated to resistance, called kdr (knockdown) mutations. Results Genome scans identified three L. salmonis Nav1 homologues, LsNav1.1, LsNav1.2 and LsNav1.3. Arthropod kdr mutations map to specific Nav1 regions within domains DI‐III, namely segments S5 and S6 and the linker helix connecting S4 and S5. The above channel regions were amplified by RT‐PCR and sequenced in deltamethrin‐susceptible and deltamethrin‐resistant L. salmonis. While LsNav1.1 and LsNav1.2 lacked nucleotide polymorphisms showing association to resistance, LsNav1.3 showed a non‐synonymous mutation in S5 of DII occurring in deltamethrin‐resistant parasites. The mutation is homologous to a previously described kdr mutation (I936V, numbering according to Musca domestica Vssc1) and was present in two pyrethroid‐resistant L. salmonis strains (allele frequencies of 0.800 and 0.357), but absent in two pyrethroid‐susceptible strains. Conclusions The present study indicates that a kdr‐mutation in LsNaV 1.3 may contribute to deltamethrin resistance in L. salmonis. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Pest Management Science 75 2 527 536 |