Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon
N-3 long chain polyunsaturated fatty acids (n-3LC-PUFA) are essential components of vertebrate membrane lipids and are crucially deficient in modern Western diets. The main human dietary source for n-3LC-PUFA is fish and seafood, particularly oily fish and over 50% of global fish production is curre...
Published in: | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/2745 2023-05-15T15:29:57+02:00 Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon Leaver, Michael Taggart, John Villeneuve, Laure Bron, James Guy, Derrick R Bishop, Stephen C Houston, Ross D Matika, Oswald Tocher, Douglas R Institute of Aquaculture Landcatch Natural Selection Ltd University of Edinburgh orcid:0000-0002-3155-0844 orcid:0000-0002-3843-9663 orcid:0000-0003-3544-0519 orcid:0000-0002-8603-9410 2011-03 application/pdf http://hdl.handle.net/1893/2745 https://doi.org/10.1016/j.cbd.2010.04.002 http://www.sciencedirect.com/science/journal/1744117X http://dspace.stir.ac.uk/bitstream/1893/2745/1/MLeaverGIA.pdf en eng Elsevier Leaver M, Taggart J, Villeneuve L, Bron J, Guy DR, Bishop SC, Houston RD, Matika O & Tocher DR (2011) Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 6 (1), pp. 62-69. http://www.sciencedirect.com/science/journal/1744117X; https://doi.org/10.1016/j.cbd.2010.04.002 http://hdl.handle.net/1893/2745 doi:10.1016/j.cbd.2010.04.002 20451480 http://www.sciencedirect.com/science/journal/1744117X WOS:000286712700009 2-s2.0-79251642246 892962 http://dspace.stir.ac.uk/bitstream/1893/2745/1/MLeaverGIA.pdf Published in Comparative Biochemistry and Physiology Part D: Genomics and Proteomics by Elsevier.; Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, Volume 6, Issue 1, March 2011, pp. 62 - 69. Atlantic salmon flesh n-3 long-chain polyunsaturated fatty acids trait heritibility microarray liver Fishes Nutrition Requirements Journal Article AM - Accepted Manuscript 2011 ftunivstirling https://doi.org/10.1016/j.cbd.2010.04.002 2022-06-13T18:43:46Z N-3 long chain polyunsaturated fatty acids (n-3LC-PUFA) are essential components of vertebrate membrane lipids and are crucially deficient in modern Western diets. The main human dietary source for n-3LC-PUFA is fish and seafood, particularly oily fish and over 50% of global fish production is currently supplied by aquaculture. However, increasing pressure to include vegetable oils, which are devoid of n-3LC-PUFA, in aquaculture feeds reduces the content of these crucial nutrients in farmed fish flesh. The aim of this study was to measure the heritability and infer mechanisms determining flesh n-3LC-PUFA content in Atlantic salmon. This was achieved by analysing flesh lipid parameters in 48 families of Atlantic salmon, and by measuring differences in hepatic mRNA expression in families with high and low flesh n-3LC-PUFA. The results show that flesh n-3LC-PUFA level is a highly heritable trait (h2 = 0.77±0.14) and indicate the involvement of increased lipid transport, most likely in the form of very low density lipoprotein (VLDL) from liver. This increase in lipid transport may be associated with increased activity of a transcription factor, hepatic nuclear factor 4α (HNF4α), possibly as a result of family differences in transforming growth factor β1 (Tgfβ1) signalling. This study paves the way for identification of quantitative trait loci and gene interaction networks that are associated with levels of n-3LC-PUFA in fish flesh. Such markers can be used to assist the sustainable production of Atlantic salmon and provide optimal levels of critical nutrients for human consumers. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 6 1 62 69 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon flesh n-3 long-chain polyunsaturated fatty acids trait heritibility microarray liver Fishes Nutrition Requirements |
spellingShingle |
Atlantic salmon flesh n-3 long-chain polyunsaturated fatty acids trait heritibility microarray liver Fishes Nutrition Requirements Leaver, Michael Taggart, John Villeneuve, Laure Bron, James Guy, Derrick R Bishop, Stephen C Houston, Ross D Matika, Oswald Tocher, Douglas R Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon |
topic_facet |
Atlantic salmon flesh n-3 long-chain polyunsaturated fatty acids trait heritibility microarray liver Fishes Nutrition Requirements |
description |
N-3 long chain polyunsaturated fatty acids (n-3LC-PUFA) are essential components of vertebrate membrane lipids and are crucially deficient in modern Western diets. The main human dietary source for n-3LC-PUFA is fish and seafood, particularly oily fish and over 50% of global fish production is currently supplied by aquaculture. However, increasing pressure to include vegetable oils, which are devoid of n-3LC-PUFA, in aquaculture feeds reduces the content of these crucial nutrients in farmed fish flesh. The aim of this study was to measure the heritability and infer mechanisms determining flesh n-3LC-PUFA content in Atlantic salmon. This was achieved by analysing flesh lipid parameters in 48 families of Atlantic salmon, and by measuring differences in hepatic mRNA expression in families with high and low flesh n-3LC-PUFA. The results show that flesh n-3LC-PUFA level is a highly heritable trait (h2 = 0.77±0.14) and indicate the involvement of increased lipid transport, most likely in the form of very low density lipoprotein (VLDL) from liver. This increase in lipid transport may be associated with increased activity of a transcription factor, hepatic nuclear factor 4α (HNF4α), possibly as a result of family differences in transforming growth factor β1 (Tgfβ1) signalling. This study paves the way for identification of quantitative trait loci and gene interaction networks that are associated with levels of n-3LC-PUFA in fish flesh. Such markers can be used to assist the sustainable production of Atlantic salmon and provide optimal levels of critical nutrients for human consumers. |
author2 |
Institute of Aquaculture Landcatch Natural Selection Ltd University of Edinburgh orcid:0000-0002-3155-0844 orcid:0000-0002-3843-9663 orcid:0000-0003-3544-0519 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Leaver, Michael Taggart, John Villeneuve, Laure Bron, James Guy, Derrick R Bishop, Stephen C Houston, Ross D Matika, Oswald Tocher, Douglas R |
author_facet |
Leaver, Michael Taggart, John Villeneuve, Laure Bron, James Guy, Derrick R Bishop, Stephen C Houston, Ross D Matika, Oswald Tocher, Douglas R |
author_sort |
Leaver, Michael |
title |
Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon |
title_short |
Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon |
title_full |
Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon |
title_fullStr |
Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon |
title_full_unstemmed |
Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon |
title_sort |
heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of atlantic salmon |
publisher |
Elsevier |
publishDate |
2011 |
url |
http://hdl.handle.net/1893/2745 https://doi.org/10.1016/j.cbd.2010.04.002 http://www.sciencedirect.com/science/journal/1744117X http://dspace.stir.ac.uk/bitstream/1893/2745/1/MLeaverGIA.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Leaver M, Taggart J, Villeneuve L, Bron J, Guy DR, Bishop SC, Houston RD, Matika O & Tocher DR (2011) Heritability and mechanisms of n-3 long chain polyunsaturated fatty acid deposition in the flesh of Atlantic salmon. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 6 (1), pp. 62-69. http://www.sciencedirect.com/science/journal/1744117X; https://doi.org/10.1016/j.cbd.2010.04.002 http://hdl.handle.net/1893/2745 doi:10.1016/j.cbd.2010.04.002 20451480 http://www.sciencedirect.com/science/journal/1744117X WOS:000286712700009 2-s2.0-79251642246 892962 http://dspace.stir.ac.uk/bitstream/1893/2745/1/MLeaverGIA.pdf |
op_rights |
Published in Comparative Biochemistry and Physiology Part D: Genomics and Proteomics by Elsevier.; Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, Volume 6, Issue 1, March 2011, pp. 62 - 69. |
op_doi |
https://doi.org/10.1016/j.cbd.2010.04.002 |
container_title |
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics |
container_volume |
6 |
container_issue |
1 |
container_start_page |
62 |
op_container_end_page |
69 |
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