Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon
Atlantic salmon migrates from rivers to sea to feed, grow and develop gonads before returning to spawn in freshwater. The transition to marine habitats is associated with dramatic changes in the environment, including water salinity, exposure to pathogens and shift in dietary lipid availability. Man...
Published in: | Molecular Ecology |
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Language: | English |
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Online Access: | http://hdl.handle.net/1893/27001 https://doi.org/10.1111/mec.14533 http://dspace.stir.ac.uk/bitstream/1893/27001/1/Harvey_etal_MolEcol_submission_revised_final.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/27001 2023-05-15T15:30:57+02:00 Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon Gillard, Gareth Harvey, Thomas Gjuvsland, Arne Jin, Yang Thomassen, Magny Lien, Sigbjorn Leaver, Michael Torgerson, Jacob Hvidsten, Torgeir Vik, Jon Olav Sandve, Simen Norwegian University of Life Sciences Institute of Aquaculture orcid:0000-0002-3155-0844 2018-03-31 application/pdf http://hdl.handle.net/1893/27001 https://doi.org/10.1111/mec.14533 http://dspace.stir.ac.uk/bitstream/1893/27001/1/Harvey_etal_MolEcol_submission_revised_final.pdf en eng Wiley-Blackwell Gillard G, Harvey T, Gjuvsland A, Jin Y, Thomassen M, Lien S, Leaver M, Torgerson J, Hvidsten T, Vik JO & Sandve S (2018) Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon. Molecular Ecology, 27 (5), pp. 1200-1213. https://doi.org/10.1111/mec.14533 http://hdl.handle.net/1893/27001 doi:10.1111/mec.14533 29431879 WOS:000429575900009 2-s2.0-85043467110 877864 http://dspace.stir.ac.uk/bitstream/1893/27001/1/Harvey_etal_MolEcol_submission_revised_final.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Gillard G, Harvey TN, Gjuvsland A, et al. Life‐stage‐associated remodelling of lipid metabolism regulation in Atlantic salmon. Molecular Ecology 2018;27:1200–1213, which has been published in final form at https://doi.org/10.1111/mec.14533. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving. [Harvey_etal_MolEcol_submission_revised_final.pdf] Publisher requires embargo of 12 months after formal publication. adaptation fish life stage metabolism transcriptomics Journal Article AM - Accepted Manuscript 2018 ftunivstirling https://doi.org/10.1111/mec.14533 2022-06-13T18:42:51Z Atlantic salmon migrates from rivers to sea to feed, grow and develop gonads before returning to spawn in freshwater. The transition to marine habitats is associated with dramatic changes in the environment, including water salinity, exposure to pathogens and shift in dietary lipid availability. Many changes in physiology and metabolism occur across this life-stage transition, but little is known about the molecular nature of these changes. Here, we use a long-term feeding experiment to study transcriptional regulation of lipid metabolism in Atlantic salmon gut and liver in both fresh- and saltwater. We find that lipid metabolism becomes significantly less plastic to differences in dietary lipid composition when salmon transitions to saltwater and experiences increased dietary lipid availability. Expression of genes in liver relating to lipogenesis and lipid transport decreases overall and becomes less responsive to diet, while genes for lipid uptake in gut become more highly expressed. Finally, analyses of evolutionary consequences of the salmonid-specific whole-genome duplication on lipid metabolism reveal several pathways with significantly different (p < .05) duplicate retention or duplicate regulatory conservation. We also find a limited number of cases where the whole-genome duplication has resulted in an increased gene dosage. In conclusion, we find variable and pathway-specific effects of the salmonid genome duplication on lipid metabolism genes. A clear life-stage-associated shift in lipid metabolism regulation is evident, and we hypothesize this to be, at least partly, driven by nondietary factors such as the preparatory remodelling of gene regulation and physiology prior to sea migration. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Molecular Ecology 27 5 1200 1213 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
adaptation fish life stage metabolism transcriptomics |
spellingShingle |
adaptation fish life stage metabolism transcriptomics Gillard, Gareth Harvey, Thomas Gjuvsland, Arne Jin, Yang Thomassen, Magny Lien, Sigbjorn Leaver, Michael Torgerson, Jacob Hvidsten, Torgeir Vik, Jon Olav Sandve, Simen Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon |
topic_facet |
adaptation fish life stage metabolism transcriptomics |
description |
Atlantic salmon migrates from rivers to sea to feed, grow and develop gonads before returning to spawn in freshwater. The transition to marine habitats is associated with dramatic changes in the environment, including water salinity, exposure to pathogens and shift in dietary lipid availability. Many changes in physiology and metabolism occur across this life-stage transition, but little is known about the molecular nature of these changes. Here, we use a long-term feeding experiment to study transcriptional regulation of lipid metabolism in Atlantic salmon gut and liver in both fresh- and saltwater. We find that lipid metabolism becomes significantly less plastic to differences in dietary lipid composition when salmon transitions to saltwater and experiences increased dietary lipid availability. Expression of genes in liver relating to lipogenesis and lipid transport decreases overall and becomes less responsive to diet, while genes for lipid uptake in gut become more highly expressed. Finally, analyses of evolutionary consequences of the salmonid-specific whole-genome duplication on lipid metabolism reveal several pathways with significantly different (p < .05) duplicate retention or duplicate regulatory conservation. We also find a limited number of cases where the whole-genome duplication has resulted in an increased gene dosage. In conclusion, we find variable and pathway-specific effects of the salmonid genome duplication on lipid metabolism genes. A clear life-stage-associated shift in lipid metabolism regulation is evident, and we hypothesize this to be, at least partly, driven by nondietary factors such as the preparatory remodelling of gene regulation and physiology prior to sea migration. |
author2 |
Norwegian University of Life Sciences Institute of Aquaculture orcid:0000-0002-3155-0844 |
format |
Article in Journal/Newspaper |
author |
Gillard, Gareth Harvey, Thomas Gjuvsland, Arne Jin, Yang Thomassen, Magny Lien, Sigbjorn Leaver, Michael Torgerson, Jacob Hvidsten, Torgeir Vik, Jon Olav Sandve, Simen |
author_facet |
Gillard, Gareth Harvey, Thomas Gjuvsland, Arne Jin, Yang Thomassen, Magny Lien, Sigbjorn Leaver, Michael Torgerson, Jacob Hvidsten, Torgeir Vik, Jon Olav Sandve, Simen |
author_sort |
Gillard, Gareth |
title |
Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon |
title_short |
Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon |
title_full |
Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon |
title_fullStr |
Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon |
title_full_unstemmed |
Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon |
title_sort |
life-stage-associated remodelling of lipid metabolism regulation in atlantic salmon |
publisher |
Wiley-Blackwell |
publishDate |
2018 |
url |
http://hdl.handle.net/1893/27001 https://doi.org/10.1111/mec.14533 http://dspace.stir.ac.uk/bitstream/1893/27001/1/Harvey_etal_MolEcol_submission_revised_final.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Gillard G, Harvey T, Gjuvsland A, Jin Y, Thomassen M, Lien S, Leaver M, Torgerson J, Hvidsten T, Vik JO & Sandve S (2018) Life-stage-associated remodelling of lipid metabolism regulation in Atlantic salmon. Molecular Ecology, 27 (5), pp. 1200-1213. https://doi.org/10.1111/mec.14533 http://hdl.handle.net/1893/27001 doi:10.1111/mec.14533 29431879 WOS:000429575900009 2-s2.0-85043467110 877864 http://dspace.stir.ac.uk/bitstream/1893/27001/1/Harvey_etal_MolEcol_submission_revised_final.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Gillard G, Harvey TN, Gjuvsland A, et al. Life‐stage‐associated remodelling of lipid metabolism regulation in Atlantic salmon. Molecular Ecology 2018;27:1200–1213, which has been published in final form at https://doi.org/10.1111/mec.14533. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving. [Harvey_etal_MolEcol_submission_revised_final.pdf] Publisher requires embargo of 12 months after formal publication. |
op_doi |
https://doi.org/10.1111/mec.14533 |
container_title |
Molecular Ecology |
container_volume |
27 |
container_issue |
5 |
container_start_page |
1200 |
op_container_end_page |
1213 |
_version_ |
1766361435256913920 |