The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish
A fever, or increased body temperature, is a symptom of inflammation, which is a complex defence reaction of the organism to pathogenic infections. After pathogens enter the body, immune cells secrete a number of agents, the functions of which stimulate the body to develop a functional immune and fe...
Published in: | Brain, Behavior, and Immunity |
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Main Authors: | , , , , , , , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2018
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Online Access: | http://hdl.handle.net/1893/26851 https://doi.org/10.1016/j.bbi.2018.03.023 http://dspace.stir.ac.uk/bitstream/1893/26851/1/1-s2.0-S088915911830076X-main.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/26851 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Ectotherm behavioural fever cytokine TRP channels virus |
spellingShingle |
Ectotherm behavioural fever cytokine TRP channels virus Boltana, Sebastian Sanhueza, Nataly Donoso, Andrea Aguilar, Andrea Crespo, Diego Vergara, Daniela Arriagada, Gabriel Morales-Lange, Byron Mercado, Luis Rey, Sonia Tort, Lluis MacKenzie, Simon The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish |
topic_facet |
Ectotherm behavioural fever cytokine TRP channels virus |
description |
A fever, or increased body temperature, is a symptom of inflammation, which is a complex defence reaction of the organism to pathogenic infections. After pathogens enter the body, immune cells secrete a number of agents, the functions of which stimulate the body to develop a functional immune and fever response. In mammals it is known that \{PGE2\} is the principal mediator of fever. The extent to which \{PGE2\} and other pro-inflammatory cytokines such as TNF-α, IL-6, or IL-1β could be involved in the induction of behavioural fever in fish remains to be clarified. Several members of the transient receptor potential (TRP) family of ion channels have been implicated as transducers of thermal stimuli, including \{TRPV1\} and TRPV2, which are activated by heat. Here we show that members of the \{TRP\} family, \{TRPV1\} and TRPV4, may participate in the coordination of temperature sensing during the behavioural fever. To examine the behavioral fever mechanism in Salmo salar an infection with IPNV, infectious pancreatic necrosis virus, was carried out by an immersion challenge with 10 ×105 PFU/mL-1 of IPNV. Behavioural fever impacted upon the expression levels of both \{TRPV1\} and \{TRPV4\} mRNAs after the viral challenge and revealed a juxtaposed regulation of \{TRPV\} channels. Our results suggest that an increase in the mRNA abundance of \{TRPV1\} is tightly correlated with a significant elevation in the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α and PGE2) in the Pre-Optic Area (POA) and cytokine release in plasma. Together, these data indicate that the reduction of \{TRPV4\} expression during behavioural fever may contribute to the onset of behavioural fever influencing movement toward higher water temperatures. Our data also suggest an effect of \{TRPV\} channels in the regulation of behavioural fever through activation of \{EP3\} receptors in the central nervous system by \{PGE2\} induced by plasma-borne cytokines. These results highlight for first time in mobile ectotherms the key role of ... |
author2 |
Fondo Nacional de Desarrollo Científico y Tecnológico Universidad de Concepción Consejo Nacional de Innovación, Ciencia y Tecnología University of Concepcion Utrecht University Pontifical Catholic University of Valparaiso Institute of Aquaculture Universitat Autonoma de Barcelona orcid:0000-0002-3406-3291 orcid:0000-0003-1845-6826 |
format |
Article in Journal/Newspaper |
author |
Boltana, Sebastian Sanhueza, Nataly Donoso, Andrea Aguilar, Andrea Crespo, Diego Vergara, Daniela Arriagada, Gabriel Morales-Lange, Byron Mercado, Luis Rey, Sonia Tort, Lluis MacKenzie, Simon |
author_facet |
Boltana, Sebastian Sanhueza, Nataly Donoso, Andrea Aguilar, Andrea Crespo, Diego Vergara, Daniela Arriagada, Gabriel Morales-Lange, Byron Mercado, Luis Rey, Sonia Tort, Lluis MacKenzie, Simon |
author_sort |
Boltana, Sebastian |
title |
The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish |
title_short |
The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish |
title_full |
The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish |
title_fullStr |
The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish |
title_full_unstemmed |
The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish |
title_sort |
expression of trpv channels, prostaglandin e2 and pro-inflammatory cytokines during behavioural fever in fish |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://hdl.handle.net/1893/26851 https://doi.org/10.1016/j.bbi.2018.03.023 http://dspace.stir.ac.uk/bitstream/1893/26851/1/1-s2.0-S088915911830076X-main.pdf |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
Boltana S, Sanhueza N, Donoso A, Aguilar A, Crespo D, Vergara D, Arriagada G, Morales-Lange B, Mercado L, Rey S, Tort L & MacKenzie S (2018) The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish. Brain, Behavior, and Immunity, 71, pp. 169-181. https://doi.org/10.1016/j.bbi.2018.03.023 http://hdl.handle.net/1893/26851 doi:10.1016/j.bbi.2018.03.023 29574261 WOS:000437386800019 2-s2.0-85044589317 878973 http://dspace.stir.ac.uk/bitstream/1893/26851/1/1-s2.0-S088915911830076X-main.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Boltana S, Sanhueza N, Donoso A, Aguilar A, Crespo D, Vergara D, Arriagada G, Morales-Lange B, Mercado L, Rey S, Tort L & MacKenzie S (2018) The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish, Brain, Behavior, and Immunity, 71, pp. 169-181. DOI:10.1016/j.bbi.2018.03.023 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ [1-s2.0-S088915911830076X-main.pdf] Publisher requires embargo of 12 months after formal publication. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.bbi.2018.03.023 |
container_title |
Brain, Behavior, and Immunity |
container_volume |
71 |
container_start_page |
169 |
op_container_end_page |
181 |
_version_ |
1766182749630103552 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/26851 2023-05-15T18:10:01+02:00 The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish Boltana, Sebastian Sanhueza, Nataly Donoso, Andrea Aguilar, Andrea Crespo, Diego Vergara, Daniela Arriagada, Gabriel Morales-Lange, Byron Mercado, Luis Rey, Sonia Tort, Lluis MacKenzie, Simon Fondo Nacional de Desarrollo Científico y Tecnológico Universidad de Concepción Consejo Nacional de Innovación, Ciencia y Tecnología University of Concepcion Utrecht University Pontifical Catholic University of Valparaiso Institute of Aquaculture Universitat Autonoma de Barcelona orcid:0000-0002-3406-3291 orcid:0000-0003-1845-6826 2018-07-31 application/pdf http://hdl.handle.net/1893/26851 https://doi.org/10.1016/j.bbi.2018.03.023 http://dspace.stir.ac.uk/bitstream/1893/26851/1/1-s2.0-S088915911830076X-main.pdf en eng Elsevier Boltana S, Sanhueza N, Donoso A, Aguilar A, Crespo D, Vergara D, Arriagada G, Morales-Lange B, Mercado L, Rey S, Tort L & MacKenzie S (2018) The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish. Brain, Behavior, and Immunity, 71, pp. 169-181. https://doi.org/10.1016/j.bbi.2018.03.023 http://hdl.handle.net/1893/26851 doi:10.1016/j.bbi.2018.03.023 29574261 WOS:000437386800019 2-s2.0-85044589317 878973 http://dspace.stir.ac.uk/bitstream/1893/26851/1/1-s2.0-S088915911830076X-main.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Boltana S, Sanhueza N, Donoso A, Aguilar A, Crespo D, Vergara D, Arriagada G, Morales-Lange B, Mercado L, Rey S, Tort L & MacKenzie S (2018) The expression of TRPV channels, prostaglandin E2 and pro-inflammatory cytokines during behavioural fever in fish, Brain, Behavior, and Immunity, 71, pp. 169-181. DOI:10.1016/j.bbi.2018.03.023 © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ [1-s2.0-S088915911830076X-main.pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND Ectotherm behavioural fever cytokine TRP channels virus Journal Article AM - Accepted Manuscript 2018 ftunivstirling https://doi.org/10.1016/j.bbi.2018.03.023 2022-06-13T18:45:26Z A fever, or increased body temperature, is a symptom of inflammation, which is a complex defence reaction of the organism to pathogenic infections. After pathogens enter the body, immune cells secrete a number of agents, the functions of which stimulate the body to develop a functional immune and fever response. In mammals it is known that \{PGE2\} is the principal mediator of fever. The extent to which \{PGE2\} and other pro-inflammatory cytokines such as TNF-α, IL-6, or IL-1β could be involved in the induction of behavioural fever in fish remains to be clarified. Several members of the transient receptor potential (TRP) family of ion channels have been implicated as transducers of thermal stimuli, including \{TRPV1\} and TRPV2, which are activated by heat. Here we show that members of the \{TRP\} family, \{TRPV1\} and TRPV4, may participate in the coordination of temperature sensing during the behavioural fever. To examine the behavioral fever mechanism in Salmo salar an infection with IPNV, infectious pancreatic necrosis virus, was carried out by an immersion challenge with 10 ×105 PFU/mL-1 of IPNV. Behavioural fever impacted upon the expression levels of both \{TRPV1\} and \{TRPV4\} mRNAs after the viral challenge and revealed a juxtaposed regulation of \{TRPV\} channels. Our results suggest that an increase in the mRNA abundance of \{TRPV1\} is tightly correlated with a significant elevation in the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α and PGE2) in the Pre-Optic Area (POA) and cytokine release in plasma. Together, these data indicate that the reduction of \{TRPV4\} expression during behavioural fever may contribute to the onset of behavioural fever influencing movement toward higher water temperatures. Our data also suggest an effect of \{TRPV\} channels in the regulation of behavioural fever through activation of \{EP3\} receptors in the central nervous system by \{PGE2\} induced by plasma-borne cytokines. These results highlight for first time in mobile ectotherms the key role of ... Article in Journal/Newspaper Salmo salar University of Stirling: Stirling Digital Research Repository Brain, Behavior, and Immunity 71 169 181 |