Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar
Background To ensure sustainability of aquaculture, plant-based ingredients are being used in feeds to replace marine-derived products. However, plants contain secondary metabolites which can affect food intake and nutrient utilisation of fish. The application of nutritional stimuli during early dev...
Published in: | BMC Genomics |
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Format: | Article in Journal/Newspaper |
Language: | English |
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BioMed Central
2017
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Online Access: | http://hdl.handle.net/1893/26182 https://doi.org/10.1186/s12864-017-4264-7 http://dspace.stir.ac.uk/bitstream/1893/26182/1/s12864-017-4264-7.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/26182 |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
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ftunivstirling |
language |
English |
topic |
Atlantic salmon nutritional programming aquaculture microarray liver transcriptome plant-based feeds |
spellingShingle |
Atlantic salmon nutritional programming aquaculture microarray liver transcriptome plant-based feeds Vera, LM Metochis, Christoforos Taylor, John Clarkson, Michael Skjaerven, Kaja H Migaud, Herve Tocher, Douglas R Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar |
topic_facet |
Atlantic salmon nutritional programming aquaculture microarray liver transcriptome plant-based feeds |
description |
Background To ensure sustainability of aquaculture, plant-based ingredients are being used in feeds to replace marine-derived products. However, plants contain secondary metabolites which can affect food intake and nutrient utilisation of fish. The application of nutritional stimuli during early development can induce long-term changes in animal physiology. Recently, we successfully used this approach to improve the utilisation of plant-based diets in diploid and triploid Atlantic salmon. In the present study we explored the molecular mechanisms occurring in the liver of salmon when challenged with a plant-based diet in order to determine the metabolic processes affected, and the effect of ploidy. Results Microarray analysis revealed that nutritional history had a major impact on the expression of genes. Key pathways of intermediary metabolism were up-regulated, including oxidative phosphorylation, pyruvate metabolism, TCA cycle, glycolysis and fatty acid metabolism. Other differentially expressed pathways affected by diet included protein processing in endoplasmic reticulum, RNA transport, endocytosis and purine metabolism. The interaction between diet and ploidy also had an effect on the hepatic transcriptome of salmon. The biological pathways with the highest number of genes affected by this interaction were related to gene transcription and translation, and cell processes such as proliferation, differentiation, communication and membrane trafficking. Conclusions The present study revealed that nutritional programming induced changes in a large number of metabolic processes in Atlantic salmon, which may be associated with the improved fish performance and nutrient utilisation demonstrated previously. In addition, differences between diploid and triploid salmon were found, supporting recent data that indicate nutritional requirements of triploid salmon may differ from those of their diploid counterparts. |
author2 |
European Commission Institute of Aquaculture University of Stirling National Institute of Nutrition and Seafood Research (NIFES) orcid:0000-0003-0999-055X orcid:0000-0002-3299-0630 orcid:0000-0003-4370-7922 orcid:0000-0002-5404-7512 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Vera, LM Metochis, Christoforos Taylor, John Clarkson, Michael Skjaerven, Kaja H Migaud, Herve Tocher, Douglas R |
author_facet |
Vera, LM Metochis, Christoforos Taylor, John Clarkson, Michael Skjaerven, Kaja H Migaud, Herve Tocher, Douglas R |
author_sort |
Vera, LM |
title |
Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar |
title_short |
Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar |
title_full |
Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar |
title_fullStr |
Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar |
title_full_unstemmed |
Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar |
title_sort |
early nutritional programming affects liver transcriptome in diploid and triploid atlantic salmon, salmo salar |
publisher |
BioMed Central |
publishDate |
2017 |
url |
http://hdl.handle.net/1893/26182 https://doi.org/10.1186/s12864-017-4264-7 http://dspace.stir.ac.uk/bitstream/1893/26182/1/s12864-017-4264-7.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Vera L, Metochis C, Taylor J, Clarkson M, Skjaerven KH, Migaud H & Tocher DR (2017) Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar. BMC Genomics, 18 (1), Art. No.: 886. https://doi.org/10.1186/s12864-017-4264-7 ARRAINA KBBE-2001-5-288925 ARRAINA 886 http://hdl.handle.net/1893/26182 doi:10.1186/s12864-017-4264-7 29149845 WOS:000415621800001 510547 http://dspace.stir.ac.uk/bitstream/1893/26182/1/s12864-017-4264-7.pdf |
op_rights |
© The Author(s). 2017 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s12864-017-4264-7 |
container_title |
BMC Genomics |
container_volume |
18 |
container_issue |
1 |
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1766360970208215040 |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/26182 2023-05-15T15:30:31+02:00 Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar Vera, LM Metochis, Christoforos Taylor, John Clarkson, Michael Skjaerven, Kaja H Migaud, Herve Tocher, Douglas R European Commission Institute of Aquaculture University of Stirling National Institute of Nutrition and Seafood Research (NIFES) orcid:0000-0003-0999-055X orcid:0000-0002-3299-0630 orcid:0000-0003-4370-7922 orcid:0000-0002-5404-7512 orcid:0000-0002-8603-9410 2017-11-17 application/pdf http://hdl.handle.net/1893/26182 https://doi.org/10.1186/s12864-017-4264-7 http://dspace.stir.ac.uk/bitstream/1893/26182/1/s12864-017-4264-7.pdf en eng BioMed Central Vera L, Metochis C, Taylor J, Clarkson M, Skjaerven KH, Migaud H & Tocher DR (2017) Early nutritional programming affects liver transcriptome in diploid and triploid Atlantic salmon, Salmo salar. BMC Genomics, 18 (1), Art. No.: 886. https://doi.org/10.1186/s12864-017-4264-7 ARRAINA KBBE-2001-5-288925 ARRAINA 886 http://hdl.handle.net/1893/26182 doi:10.1186/s12864-017-4264-7 29149845 WOS:000415621800001 510547 http://dspace.stir.ac.uk/bitstream/1893/26182/1/s12864-017-4264-7.pdf © The Author(s). 2017 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://creativecommons.org/licenses/by/4.0/ CC0 PDM CC-BY Atlantic salmon nutritional programming aquaculture microarray liver transcriptome plant-based feeds Journal Article VoR - Version of Record 2017 ftunivstirling https://doi.org/10.1186/s12864-017-4264-7 2022-06-13T18:46:11Z Background To ensure sustainability of aquaculture, plant-based ingredients are being used in feeds to replace marine-derived products. However, plants contain secondary metabolites which can affect food intake and nutrient utilisation of fish. The application of nutritional stimuli during early development can induce long-term changes in animal physiology. Recently, we successfully used this approach to improve the utilisation of plant-based diets in diploid and triploid Atlantic salmon. In the present study we explored the molecular mechanisms occurring in the liver of salmon when challenged with a plant-based diet in order to determine the metabolic processes affected, and the effect of ploidy. Results Microarray analysis revealed that nutritional history had a major impact on the expression of genes. Key pathways of intermediary metabolism were up-regulated, including oxidative phosphorylation, pyruvate metabolism, TCA cycle, glycolysis and fatty acid metabolism. Other differentially expressed pathways affected by diet included protein processing in endoplasmic reticulum, RNA transport, endocytosis and purine metabolism. The interaction between diet and ploidy also had an effect on the hepatic transcriptome of salmon. The biological pathways with the highest number of genes affected by this interaction were related to gene transcription and translation, and cell processes such as proliferation, differentiation, communication and membrane trafficking. Conclusions The present study revealed that nutritional programming induced changes in a large number of metabolic processes in Atlantic salmon, which may be associated with the improved fish performance and nutrient utilisation demonstrated previously. In addition, differences between diploid and triploid salmon were found, supporting recent data that indicate nutritional requirements of triploid salmon may differ from those of their diploid counterparts. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository BMC Genomics 18 1 |