In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments
In this study, microbial community dynamics were assessed within a simple invitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic t...
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ftunivstirling:oai:dspace.stir.ac.uk:1893/25845 2023-05-15T15:31:24+02:00 In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments Zarkasi, Kamarul Zaman Taylor, Richard S Glencross, Brett Abell, Guy C J Tamplin, Mark L Bowman, John P University of Science, Malaysia (USM) Commonwealth Scientific and Industrial Research Organisation (CSIRO) Institute of Aquaculture University of Tasmania orcid:0000-0003-1167-8530 2017-10 application/pdf http://hdl.handle.net/1893/25845 https://doi.org/10.1016/j.resmic.2017.07.003 http://dspace.stir.ac.uk/bitstream/1893/25845/1/Zarkasi_et_a_2017-GOA_version.pdf en eng Elsevier Zarkasi KZ, Taylor RS, Glencross B, Abell GCJ, Tamplin ML & Bowman JP (2017) In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments. Research in Microbiology, 168 (8), pp. 751-759. https://doi.org/10.1016/j.resmic.2017.07.003 http://hdl.handle.net/1893/25845 doi:10.1016/j.resmic.2017.07.003 28728852 WOS:000413662700007 2-s2.0-85026826522 520938 http://dspace.stir.ac.uk/bitstream/1893/25845/1/Zarkasi_et_a_2017-GOA_version.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Zarkasi KZ, Taylor RS, Glencross B, Abell GCJ, Tamplin ML & Bowman JP (2017) In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments, Research in Microbiology, 168 (8), pp. 751-759. DOI:10.1016/j.resmic.2017.07.003 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2018-07-18 [Zarkasi_et_a_2017-GOA_version.pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND Intestinal bacteria In vitro model system 16S rRNA gene Dietary treatments Atlantic salmon Journal Article AM - Accepted Manuscript 2017 ftunivstirling https://doi.org/10.1016/j.resmic.2017.07.003 2022-06-13T18:44:35Z In this study, microbial community dynamics were assessed within a simple invitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic the effects of dietary variables. A total of five complete diets and two ingredients (plant meal) were tested. The total viable counts (TVCs) and sequencing data revealed that there was very clear separation between the complete diets and the plant meal treatments, suggesting a dynamic response by the allochthonous bacteria to the treatments. Automated ribosomal intergenic spacer analysis (ARISA) results showed that different diet formulations produced different patterns of fragments, with no separation between the complete diets. However, plant-based protein ingredients were clearly separated from the other treatments. 16S rRNA Illumina-based sequencing analysis showed that members of the genera Aliivibrio,Vibrioand Photobacteriumbecame predominant for all complete diets treatments. The plant-based protein ingredient treatments only sustained weak growth of the genusSphingomonas. Invitro based testing of diets could be a useful strategy to determine the potential impact of either complete feeds or ingredients on major fish gastrointestinal tract microbiome members. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Research in Microbiology 168 8 751 759 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Intestinal bacteria In vitro model system 16S rRNA gene Dietary treatments Atlantic salmon |
spellingShingle |
Intestinal bacteria In vitro model system 16S rRNA gene Dietary treatments Atlantic salmon Zarkasi, Kamarul Zaman Taylor, Richard S Glencross, Brett Abell, Guy C J Tamplin, Mark L Bowman, John P In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments |
topic_facet |
Intestinal bacteria In vitro model system 16S rRNA gene Dietary treatments Atlantic salmon |
description |
In this study, microbial community dynamics were assessed within a simple invitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic the effects of dietary variables. A total of five complete diets and two ingredients (plant meal) were tested. The total viable counts (TVCs) and sequencing data revealed that there was very clear separation between the complete diets and the plant meal treatments, suggesting a dynamic response by the allochthonous bacteria to the treatments. Automated ribosomal intergenic spacer analysis (ARISA) results showed that different diet formulations produced different patterns of fragments, with no separation between the complete diets. However, plant-based protein ingredients were clearly separated from the other treatments. 16S rRNA Illumina-based sequencing analysis showed that members of the genera Aliivibrio,Vibrioand Photobacteriumbecame predominant for all complete diets treatments. The plant-based protein ingredient treatments only sustained weak growth of the genusSphingomonas. Invitro based testing of diets could be a useful strategy to determine the potential impact of either complete feeds or ingredients on major fish gastrointestinal tract microbiome members. |
author2 |
University of Science, Malaysia (USM) Commonwealth Scientific and Industrial Research Organisation (CSIRO) Institute of Aquaculture University of Tasmania orcid:0000-0003-1167-8530 |
format |
Article in Journal/Newspaper |
author |
Zarkasi, Kamarul Zaman Taylor, Richard S Glencross, Brett Abell, Guy C J Tamplin, Mark L Bowman, John P |
author_facet |
Zarkasi, Kamarul Zaman Taylor, Richard S Glencross, Brett Abell, Guy C J Tamplin, Mark L Bowman, John P |
author_sort |
Zarkasi, Kamarul Zaman |
title |
In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments |
title_short |
In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments |
title_full |
In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments |
title_fullStr |
In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments |
title_full_unstemmed |
In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments |
title_sort |
in vitro characteristics of an atlantic salmon (salmo salar l.) hind gut microbial community in relation to different dietary treatments |
publisher |
Elsevier |
publishDate |
2017 |
url |
http://hdl.handle.net/1893/25845 https://doi.org/10.1016/j.resmic.2017.07.003 http://dspace.stir.ac.uk/bitstream/1893/25845/1/Zarkasi_et_a_2017-GOA_version.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Zarkasi KZ, Taylor RS, Glencross B, Abell GCJ, Tamplin ML & Bowman JP (2017) In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments. Research in Microbiology, 168 (8), pp. 751-759. https://doi.org/10.1016/j.resmic.2017.07.003 http://hdl.handle.net/1893/25845 doi:10.1016/j.resmic.2017.07.003 28728852 WOS:000413662700007 2-s2.0-85026826522 520938 http://dspace.stir.ac.uk/bitstream/1893/25845/1/Zarkasi_et_a_2017-GOA_version.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Zarkasi KZ, Taylor RS, Glencross B, Abell GCJ, Tamplin ML & Bowman JP (2017) In vitro characteristics of an Atlantic salmon (Salmo salar L.) hind gut microbial community in relation to different dietary treatments, Research in Microbiology, 168 (8), pp. 751-759. DOI:10.1016/j.resmic.2017.07.003 © 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2018-07-18 [Zarkasi_et_a_2017-GOA_version.pdf] Publisher requires embargo of 12 months after formal publication. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.resmic.2017.07.003 |
container_title |
Research in Microbiology |
container_volume |
168 |
container_issue |
8 |
container_start_page |
751 |
op_container_end_page |
759 |
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1766361894129500160 |