Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals
Pharmaceuticals are pseudopersistent aquatic pollutants with unknown effects at environmentally relevant concentrations. Atlantic salmon (Salmo salar) were exposed to Acetaminophen: 54.77 ± 34.67; Atenolol: 11.08 ± 7.98, and Carbamazepine: 7.85 ± 0.13 μg·L−1for 5 days. After Acetaminophen treatment,...
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American Society for Biochemistry and Molecular Biology
2015
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Online Access: | http://hdl.handle.net/1893/25461 https://doi.org/10.1074/mcp.M114.045120 http://dspace.stir.ac.uk/bitstream/1893/25461/1/Mol%20Cell%20Proteomics-2015-Hampel-371-81.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/25461 2023-05-15T15:30:29+02:00 Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael European Commission University of Cadiz University of Seville Institute of Aquaculture orcid:0000-0002-3155-0844 2015-02-01 application/pdf http://hdl.handle.net/1893/25461 https://doi.org/10.1074/mcp.M114.045120 http://dspace.stir.ac.uk/bitstream/1893/25461/1/Mol%20Cell%20Proteomics-2015-Hampel-371-81.pdf en eng American Society for Biochemistry and Molecular Biology Hampel M, Alonso E, Aparicio I, Santos JL & Leaver M (2015) Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals. Molecular and Cellular Proteomics, 14 (2), pp. 371-381. https://doi.org/10.1074/mcp.M114.045120 SALMONPHARM MEIF-CT-2006-03961 http://hdl.handle.net/1893/25461 doi:10.1074/mcp.M114.045120 25394398 WOS:000349144000009 2-s2.0-84921903324 570483 http://dspace.stir.ac.uk/bitstream/1893/25461/1/Mol%20Cell%20Proteomics-2015-Hampel-371-81.pdf Publisher policy allows this work to be made available in this repository. Published in Molecular & Cellular Proteomics, 14, 371-381 by American Society for Biochemistry and Molecular Biology. The original publication is available at: https://doi.org/10.1074/mcp.M114.045120 Journal Article VoR - Version of Record 2015 ftunivstirling https://doi.org/10.1074/mcp.M114.045120 2022-06-13T18:43:17Z Pharmaceuticals are pseudopersistent aquatic pollutants with unknown effects at environmentally relevant concentrations. Atlantic salmon (Salmo salar) were exposed to Acetaminophen: 54.77 ± 34.67; Atenolol: 11.08 ± 7.98, and Carbamazepine: 7.85 ± 0.13 μg·L−1for 5 days. After Acetaminophen treatment, 19 proteins were differently expressed, of which 11 were significant with respect to the control group (eight up-regulated and three down-regulated). After Atenolol treatment, seven differently expressed proteins were obtained in comparison with the control, of which six could be identified (four up-regulated and two down-regulated). Carbamazepine exposure resulted in 15 differently expressed proteins compared with the control, with 10 of them identified (seven up-regulated and three down-regulated). Out of these, three features were common between Acetaminophen and Carbamazepine and one between Carbamazepine and Atenolol. One feature was common across all treatments. Principal component analysis and heat map clustering showed a clear grouping of the variability caused by the applied treatments. The obtained data suggest (1) that exposure to environmentally relevant concentrations of the pharmaceuticals alters the hepatic protein expression profile of the Atlantic salmon; and (2) the existence of treatment specific processes that may be useful for biomarker development. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Molecular & Cellular Proteomics 14 2 371 381 |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
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ftunivstirling |
language |
English |
description |
Pharmaceuticals are pseudopersistent aquatic pollutants with unknown effects at environmentally relevant concentrations. Atlantic salmon (Salmo salar) were exposed to Acetaminophen: 54.77 ± 34.67; Atenolol: 11.08 ± 7.98, and Carbamazepine: 7.85 ± 0.13 μg·L−1for 5 days. After Acetaminophen treatment, 19 proteins were differently expressed, of which 11 were significant with respect to the control group (eight up-regulated and three down-regulated). After Atenolol treatment, seven differently expressed proteins were obtained in comparison with the control, of which six could be identified (four up-regulated and two down-regulated). Carbamazepine exposure resulted in 15 differently expressed proteins compared with the control, with 10 of them identified (seven up-regulated and three down-regulated). Out of these, three features were common between Acetaminophen and Carbamazepine and one between Carbamazepine and Atenolol. One feature was common across all treatments. Principal component analysis and heat map clustering showed a clear grouping of the variability caused by the applied treatments. The obtained data suggest (1) that exposure to environmentally relevant concentrations of the pharmaceuticals alters the hepatic protein expression profile of the Atlantic salmon; and (2) the existence of treatment specific processes that may be useful for biomarker development. |
author2 |
European Commission University of Cadiz University of Seville Institute of Aquaculture orcid:0000-0002-3155-0844 |
format |
Article in Journal/Newspaper |
author |
Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael |
spellingShingle |
Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals |
author_facet |
Hampel, Miriam Alonso, Esteban Aparicio, Irene Santos, Juan Luis Leaver, Michael |
author_sort |
Hampel, Miriam |
title |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals |
title_short |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals |
title_full |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals |
title_fullStr |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals |
title_full_unstemmed |
Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals |
title_sort |
hepatic proteome analysis of atlantic salmon (salmo salar) after exposure to environmental concentrations of human pharmaceuticals |
publisher |
American Society for Biochemistry and Molecular Biology |
publishDate |
2015 |
url |
http://hdl.handle.net/1893/25461 https://doi.org/10.1074/mcp.M114.045120 http://dspace.stir.ac.uk/bitstream/1893/25461/1/Mol%20Cell%20Proteomics-2015-Hampel-371-81.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Hampel M, Alonso E, Aparicio I, Santos JL & Leaver M (2015) Hepatic Proteome Analysis of Atlantic Salmon (Salmo salar) After Exposure to Environmental Concentrations of Human Pharmaceuticals. Molecular and Cellular Proteomics, 14 (2), pp. 371-381. https://doi.org/10.1074/mcp.M114.045120 SALMONPHARM MEIF-CT-2006-03961 http://hdl.handle.net/1893/25461 doi:10.1074/mcp.M114.045120 25394398 WOS:000349144000009 2-s2.0-84921903324 570483 http://dspace.stir.ac.uk/bitstream/1893/25461/1/Mol%20Cell%20Proteomics-2015-Hampel-371-81.pdf |
op_rights |
Publisher policy allows this work to be made available in this repository. Published in Molecular & Cellular Proteomics, 14, 371-381 by American Society for Biochemistry and Molecular Biology. The original publication is available at: https://doi.org/10.1074/mcp.M114.045120 |
op_doi |
https://doi.org/10.1074/mcp.M114.045120 |
container_title |
Molecular & Cellular Proteomics |
container_volume |
14 |
container_issue |
2 |
container_start_page |
371 |
op_container_end_page |
381 |
_version_ |
1766360928488521728 |