An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry
New de novo sources of omega 3 (n-3) long chain polyunsaturated fatty acids (LC-PUFA) are required as alternatives to fish oil in aquafeeds in order to maintain adequate levels of the beneficial fatty acids, eicosapentaenoic and docosahexaenoic (EPA and DHA, respectively). The present study investig...
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Online Access: | http://hdl.handle.net/1893/25220 https://doi.org/10.1371/journal.pone.0175415 http://dspace.stir.ac.uk/bitstream/1893/25220/1/journal.pone.0175415.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/25220 2024-06-02T08:03:29+00:00 An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry Betancor, Monica Li, Keshuai Sprague, Matthew Bardal, Tora Sayanova, Olga Usher, Sarah Han, Lihua Masoval, Kjell Torrissen, Ole Napier, Johnathan A Tocher, Douglas R Olsen, Rolf Erik Institute of Aquaculture Norwegian University of Science And Technology (NTNU) Rothamsted Research BioMar AS Norwegian Institute of Marine Research orcid:0000-0003-1626-7458 orcid:0000-0002-0723-2387 orcid:0000-0002-8603-9410 2017-04-12 application/pdf http://hdl.handle.net/1893/25220 https://doi.org/10.1371/journal.pone.0175415 http://dspace.stir.ac.uk/bitstream/1893/25220/1/journal.pone.0175415.pdf en eng Public Library of Science Betancor M, Li K, Sprague M, Bardal T, Sayanova O, Usher S, Han L, Masoval K, Torrissen O, Napier JA, Tocher DR & Olsen RE (2017) An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PLoS ONE, 12 (4), Art. No.: e0175415. https://doi.org/10.1371/journal.pone.0175415 e0175415 http://hdl.handle.net/1893/25220 doi:10.1371/journal.pone.0175415 28403232 WOS:000399955200065 2-s2.0-85017478270 532746 http://dspace.stir.ac.uk/bitstream/1893/25220/1/journal.pone.0175415.pdf © 2017 Betancor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ Journal Article VoR - Version of Record 2017 ftunivstirling https://doi.org/10.1371/journal.pone.0175415 2024-05-07T04:30:11Z New de novo sources of omega 3 (n-3) long chain polyunsaturated fatty acids (LC-PUFA) are required as alternatives to fish oil in aquafeeds in order to maintain adequate levels of the beneficial fatty acids, eicosapentaenoic and docosahexaenoic (EPA and DHA, respectively). The present study investigated the use of an EPA+DHA oil derived from transgenic Camelina sativa in Atlantic salmon (Salmo salar) feeds containing low levels of fishmeal (35 %) and fish oil (10 %), reflecting current commercial formulations, to determine the impacts on tissue fatty acid profile, intestinal transcriptome, and health of farmed salmon. Post-smolt Atlantic salmon were fed for 12-weeks with one of three experimental diets containing either a blend of fish oil/rapeseed oil (FO), wild-type camelina oil (WCO) or transgenic camelina oil (DCO) as added lipid source. The DCO diet did not affect any of the fish performance or health parameters studied. Analyses of the mid and hindgut transcriptomes showed only mild effects on metabolism. Flesh of fish fed the DCO diet accumulated almost double the amount of n-3 LC-PUFA than fish fed the FO or WCO diets, indicating that these oils from transgenic oilseeds offer the opportunity to increase the n-3 LC-PUFA in farmed fish to levels comparable to those found a decade ago. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository PLOS ONE 12 4 e0175415 |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
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ftunivstirling |
language |
English |
description |
New de novo sources of omega 3 (n-3) long chain polyunsaturated fatty acids (LC-PUFA) are required as alternatives to fish oil in aquafeeds in order to maintain adequate levels of the beneficial fatty acids, eicosapentaenoic and docosahexaenoic (EPA and DHA, respectively). The present study investigated the use of an EPA+DHA oil derived from transgenic Camelina sativa in Atlantic salmon (Salmo salar) feeds containing low levels of fishmeal (35 %) and fish oil (10 %), reflecting current commercial formulations, to determine the impacts on tissue fatty acid profile, intestinal transcriptome, and health of farmed salmon. Post-smolt Atlantic salmon were fed for 12-weeks with one of three experimental diets containing either a blend of fish oil/rapeseed oil (FO), wild-type camelina oil (WCO) or transgenic camelina oil (DCO) as added lipid source. The DCO diet did not affect any of the fish performance or health parameters studied. Analyses of the mid and hindgut transcriptomes showed only mild effects on metabolism. Flesh of fish fed the DCO diet accumulated almost double the amount of n-3 LC-PUFA than fish fed the FO or WCO diets, indicating that these oils from transgenic oilseeds offer the opportunity to increase the n-3 LC-PUFA in farmed fish to levels comparable to those found a decade ago. |
author2 |
Institute of Aquaculture Norwegian University of Science And Technology (NTNU) Rothamsted Research BioMar AS Norwegian Institute of Marine Research orcid:0000-0003-1626-7458 orcid:0000-0002-0723-2387 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Betancor, Monica Li, Keshuai Sprague, Matthew Bardal, Tora Sayanova, Olga Usher, Sarah Han, Lihua Masoval, Kjell Torrissen, Ole Napier, Johnathan A Tocher, Douglas R Olsen, Rolf Erik |
spellingShingle |
Betancor, Monica Li, Keshuai Sprague, Matthew Bardal, Tora Sayanova, Olga Usher, Sarah Han, Lihua Masoval, Kjell Torrissen, Ole Napier, Johnathan A Tocher, Douglas R Olsen, Rolf Erik An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
author_facet |
Betancor, Monica Li, Keshuai Sprague, Matthew Bardal, Tora Sayanova, Olga Usher, Sarah Han, Lihua Masoval, Kjell Torrissen, Ole Napier, Johnathan A Tocher, Douglas R Olsen, Rolf Erik |
author_sort |
Betancor, Monica |
title |
An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
title_short |
An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
title_full |
An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
title_fullStr |
An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
title_full_unstemmed |
An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
title_sort |
oil containing epa and dha from transgenic camelina sativa to replace marine fish oil in feeds for atlantic salmon (salmo salar l.): effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry |
publisher |
Public Library of Science |
publishDate |
2017 |
url |
http://hdl.handle.net/1893/25220 https://doi.org/10.1371/journal.pone.0175415 http://dspace.stir.ac.uk/bitstream/1893/25220/1/journal.pone.0175415.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Betancor M, Li K, Sprague M, Bardal T, Sayanova O, Usher S, Han L, Masoval K, Torrissen O, Napier JA, Tocher DR & Olsen RE (2017) An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PLoS ONE, 12 (4), Art. No.: e0175415. https://doi.org/10.1371/journal.pone.0175415 e0175415 http://hdl.handle.net/1893/25220 doi:10.1371/journal.pone.0175415 28403232 WOS:000399955200065 2-s2.0-85017478270 532746 http://dspace.stir.ac.uk/bitstream/1893/25220/1/journal.pone.0175415.pdf |
op_rights |
© 2017 Betancor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0175415 |
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PLOS ONE |
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12 |
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4 |
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e0175415 |
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