Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta).
Cleaner fish, including ballan wrasse (Labrus bergylta) have been proposed as a sustainable solution to sea lice infestations affecting farmed Atlantic salmon (Salmo salar) globally. However, in order to become sustainable, ballan wrasse need to be farmed. This thesis investigated the establishment...
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Language: | English |
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University of Stirling
2016
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Online Access: | http://hdl.handle.net/1893/24416 http://dspace.stir.ac.uk/bitstream/1893/24416/1/Bridie%20Grant%20Final%20Thesis%2017_10_2016.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/24416 2023-05-15T15:32:01+02:00 Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). Grant, Bridie Migaud, Herve Davie, Andrew Marine Harvest (Scotland) Ltd., Scottish Sea Farms, Innovate UK (Project Ref. 81199). 2016-03-31 application/pdf http://hdl.handle.net/1893/24416 http://dspace.stir.ac.uk/bitstream/1893/24416/1/Bridie%20Grant%20Final%20Thesis%2017_10_2016.pdf en eng University of Stirling Institute of Aquaculture Grant, B., Picci, N., Davie, A., Leclercq, E., & Migaud, H., (2016). Removal of adhesiveness in naturally fertilized ballan wrasse (Labrus bergylta) eggs. Aquaculture 456, 44-49. Grant, B., Picci, N., Davie, A., Taggart, J.B., Bradley, C., Prodohl, P., Leclercq, E., & Migaud, H., Seasonal changes in broodstock spawning performance and egg quality in ballan wrasse (Labrus bergylta). Aquaculture 464, 505-514. http://hdl.handle.net/1893/24416 http://dspace.stir.ac.uk/bitstream/1893/24416/1/Bridie%20Grant%20Final%20Thesis%2017_10_2016.pdf 2022-05-31 Time needed to publish before thesis is made freely available; funder requires longer embargo 2022-06-01 Labrus bergylta cleaner fish phylogeographic structure broodstock management benthic spawning egg quality behaviour degumming Fishes Breeding Ballan wrasse Caligidae Biological control Atlantic salmon Parasties Biological control Thesis or Dissertation Doctoral Doctor of Philosophy 2016 ftunivstirling 2022-06-13T18:43:56Z Cleaner fish, including ballan wrasse (Labrus bergylta) have been proposed as a sustainable solution to sea lice infestations affecting farmed Atlantic salmon (Salmo salar) globally. However, in order to become sustainable, ballan wrasse need to be farmed. This thesis investigated the establishment of captive broodstock and protocols to optimise hatchery performance and productivity of ballan wrasse. High throughput sequencing was used to develop a panel of novel single nucleotide polymorphic markers (SNPs). These SNPs were used to investigate the phylogeographic structuring of ballan wrasse populations within northern geographic ranges including the UK and Norway. Results indicated fine scale population structuring within the UK suggesting that founder broodstock should be sourced locally to minimise the risk of genetic introgression with wild ballan wrasse. Secondly, captive breeding was benchmarked from harems to determine total egg production over the spawning season. Data quantified the spawning periodicity and seasonal changes in egg quality parameters. In addition, microsatellite markers identified the parental contribution to each spawning event of captive broodstock. Results confirmed, for the first time, the repeat-batch spawning behaviour and suggested that spawning events were single-paired matings. Furthermore, bottlenecks in commercial production were investigated including the benthic adhesive eggs and complex spawning behaviours of ballan wrasse within broodstock tanks. Experiments were conducted to optimise the spawning dynamics and egg productivity using fragmented spawning zones and coloured substrates. Finally, an effective bath treatment for removal of the adhesive gum layer of eggs using the proteolytic enzyme alcalaseĀ® was found to assist in egg disinfection and incubation. Overall, this research provides important baseline data on the management of broodstock and the optimisation of hatchery protocols to improve the commercial productivity and performance of ballan wrasse for use as a ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Ballan ENVELOPE(12.203,12.203,65.945,65.945) Norway |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Labrus bergylta cleaner fish phylogeographic structure broodstock management benthic spawning egg quality behaviour degumming Fishes Breeding Ballan wrasse Caligidae Biological control Atlantic salmon Parasties Biological control |
spellingShingle |
Labrus bergylta cleaner fish phylogeographic structure broodstock management benthic spawning egg quality behaviour degumming Fishes Breeding Ballan wrasse Caligidae Biological control Atlantic salmon Parasties Biological control Grant, Bridie Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). |
topic_facet |
Labrus bergylta cleaner fish phylogeographic structure broodstock management benthic spawning egg quality behaviour degumming Fishes Breeding Ballan wrasse Caligidae Biological control Atlantic salmon Parasties Biological control |
description |
Cleaner fish, including ballan wrasse (Labrus bergylta) have been proposed as a sustainable solution to sea lice infestations affecting farmed Atlantic salmon (Salmo salar) globally. However, in order to become sustainable, ballan wrasse need to be farmed. This thesis investigated the establishment of captive broodstock and protocols to optimise hatchery performance and productivity of ballan wrasse. High throughput sequencing was used to develop a panel of novel single nucleotide polymorphic markers (SNPs). These SNPs were used to investigate the phylogeographic structuring of ballan wrasse populations within northern geographic ranges including the UK and Norway. Results indicated fine scale population structuring within the UK suggesting that founder broodstock should be sourced locally to minimise the risk of genetic introgression with wild ballan wrasse. Secondly, captive breeding was benchmarked from harems to determine total egg production over the spawning season. Data quantified the spawning periodicity and seasonal changes in egg quality parameters. In addition, microsatellite markers identified the parental contribution to each spawning event of captive broodstock. Results confirmed, for the first time, the repeat-batch spawning behaviour and suggested that spawning events were single-paired matings. Furthermore, bottlenecks in commercial production were investigated including the benthic adhesive eggs and complex spawning behaviours of ballan wrasse within broodstock tanks. Experiments were conducted to optimise the spawning dynamics and egg productivity using fragmented spawning zones and coloured substrates. Finally, an effective bath treatment for removal of the adhesive gum layer of eggs using the proteolytic enzyme alcalaseĀ® was found to assist in egg disinfection and incubation. Overall, this research provides important baseline data on the management of broodstock and the optimisation of hatchery protocols to improve the commercial productivity and performance of ballan wrasse for use as a ... |
author2 |
Migaud, Herve Davie, Andrew Marine Harvest (Scotland) Ltd., Scottish Sea Farms, Innovate UK (Project Ref. 81199). |
format |
Doctoral or Postdoctoral Thesis |
author |
Grant, Bridie |
author_facet |
Grant, Bridie |
author_sort |
Grant, Bridie |
title |
Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). |
title_short |
Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). |
title_full |
Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). |
title_fullStr |
Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). |
title_full_unstemmed |
Development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (Labrus bergylta). |
title_sort |
development of broodstock management and husbandry tools for improved hatchery performance of ballan wrasse (labrus bergylta). |
publisher |
University of Stirling |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/24416 http://dspace.stir.ac.uk/bitstream/1893/24416/1/Bridie%20Grant%20Final%20Thesis%2017_10_2016.pdf |
long_lat |
ENVELOPE(12.203,12.203,65.945,65.945) |
geographic |
Ballan Norway |
geographic_facet |
Ballan Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Grant, B., Picci, N., Davie, A., Leclercq, E., & Migaud, H., (2016). Removal of adhesiveness in naturally fertilized ballan wrasse (Labrus bergylta) eggs. Aquaculture 456, 44-49. Grant, B., Picci, N., Davie, A., Taggart, J.B., Bradley, C., Prodohl, P., Leclercq, E., & Migaud, H., Seasonal changes in broodstock spawning performance and egg quality in ballan wrasse (Labrus bergylta). Aquaculture 464, 505-514. http://hdl.handle.net/1893/24416 http://dspace.stir.ac.uk/bitstream/1893/24416/1/Bridie%20Grant%20Final%20Thesis%2017_10_2016.pdf |
op_rights |
2022-05-31 Time needed to publish before thesis is made freely available; funder requires longer embargo 2022-06-01 |
_version_ |
1766362516626079744 |