Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey
The present study is the first to evaluate lipid metabolism in first-feeding Atlantic bluefin tuna (ABT; Thunnus thynnus L.) larvae fed different live prey including enriched rotifers Brachionus plicatilis and Acartia sp. copepod nauplii from 2 days after hatch. Understanding the molecular basis of...
Published in: | Fish Physiology and Biochemistry |
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Online Access: | http://hdl.handle.net/1893/24381 https://doi.org/10.1007/s10695-016-0305-4 http://dspace.stir.ac.uk/bitstream/1893/24381/1/Betancor_etal_FishPhysiolBiochem_2017.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/24381 2023-05-15T18:49:41+02:00 Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey Betancor, Monica Ortega, Aurelio de la Gandara, Fernando Tocher, Douglas R Mourente, Gabriel Institute of Aquaculture Spanish Institute of Oceanography (IEO) University of Cadiz orcid:0000-0003-1626-7458 orcid:0000-0002-8603-9410 2017-04 application/pdf http://hdl.handle.net/1893/24381 https://doi.org/10.1007/s10695-016-0305-4 http://dspace.stir.ac.uk/bitstream/1893/24381/1/Betancor_etal_FishPhysiolBiochem_2017.pdf en eng Springer Betancor M, Ortega A, de la Gandara F, Tocher DR & Mourente G (2017) Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey. Fish Physiology and Biochemistry, 43 (2), pp. 493-516. https://doi.org/10.1007/s10695-016-0305-4 http://hdl.handle.net/1893/24381 doi:10.1007/s10695-016-0305-4 27815797 WOS:000399017500016 2-s2.0-84994376829 547287 http://dspace.stir.ac.uk/bitstream/1893/24381/1/Betancor_etal_FishPhysiolBiochem_2017.pdf © The Author(s) 2016 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. http://creativecommons.org/licenses/by/4.0/ CC-BY Bluefin tuna larvae rotifer copepods lipid content lipid classes fatty acid composition cDNA gene expression Journal Article VoR - Version of Record 2017 ftunivstirling https://doi.org/10.1007/s10695-016-0305-4 2022-06-13T18:42:22Z The present study is the first to evaluate lipid metabolism in first-feeding Atlantic bluefin tuna (ABT; Thunnus thynnus L.) larvae fed different live prey including enriched rotifers Brachionus plicatilis and Acartia sp. copepod nauplii from 2 days after hatch. Understanding the molecular basis of lipid metabolism and regulation in ABT will provide insights to optimise diet formulations for this high value species new to aquaculture. To this end, we investigated the effect of dietary lipid on whole larvae lipid class and fatty acid compositions and the expression of key genes involved in lipid metabolism in first feeding ABT larvae fed different live prey. Additionally, the expression of lipid metabolism genes in tissues of adult broodstock ABT was evaluated. Growth and survival data indicated that copepods were the best live prey for first feeding ABT, and that differences in growth performance and lipid metabolism observed between larvae from different year classes could be a consequence of broodstock nutrition. In addition, expression patterns of lipid metabolic genes observed in ABT larvae in the trials could reflect differences in lipid class and fatty acid compositions of the live prey. The lipid nutritional requirements, including essential fatty acid requirements of larval ABT during the early feeding stages are unknown and the present study represents a first step in addressing these highly relevant issues. However, further studies are required to determine nutritional requirements and understand lipid metabolism during development of ABT larvae, and to apply the knowledge to the commercial culture of this iconic species. Article in Journal/Newspaper Copepods Rotifer University of Stirling: Stirling Digital Research Repository Fish Physiology and Biochemistry 43 2 493 516 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Bluefin tuna larvae rotifer copepods lipid content lipid classes fatty acid composition cDNA gene expression |
spellingShingle |
Bluefin tuna larvae rotifer copepods lipid content lipid classes fatty acid composition cDNA gene expression Betancor, Monica Ortega, Aurelio de la Gandara, Fernando Tocher, Douglas R Mourente, Gabriel Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey |
topic_facet |
Bluefin tuna larvae rotifer copepods lipid content lipid classes fatty acid composition cDNA gene expression |
description |
The present study is the first to evaluate lipid metabolism in first-feeding Atlantic bluefin tuna (ABT; Thunnus thynnus L.) larvae fed different live prey including enriched rotifers Brachionus plicatilis and Acartia sp. copepod nauplii from 2 days after hatch. Understanding the molecular basis of lipid metabolism and regulation in ABT will provide insights to optimise diet formulations for this high value species new to aquaculture. To this end, we investigated the effect of dietary lipid on whole larvae lipid class and fatty acid compositions and the expression of key genes involved in lipid metabolism in first feeding ABT larvae fed different live prey. Additionally, the expression of lipid metabolism genes in tissues of adult broodstock ABT was evaluated. Growth and survival data indicated that copepods were the best live prey for first feeding ABT, and that differences in growth performance and lipid metabolism observed between larvae from different year classes could be a consequence of broodstock nutrition. In addition, expression patterns of lipid metabolic genes observed in ABT larvae in the trials could reflect differences in lipid class and fatty acid compositions of the live prey. The lipid nutritional requirements, including essential fatty acid requirements of larval ABT during the early feeding stages are unknown and the present study represents a first step in addressing these highly relevant issues. However, further studies are required to determine nutritional requirements and understand lipid metabolism during development of ABT larvae, and to apply the knowledge to the commercial culture of this iconic species. |
author2 |
Institute of Aquaculture Spanish Institute of Oceanography (IEO) University of Cadiz orcid:0000-0003-1626-7458 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Betancor, Monica Ortega, Aurelio de la Gandara, Fernando Tocher, Douglas R Mourente, Gabriel |
author_facet |
Betancor, Monica Ortega, Aurelio de la Gandara, Fernando Tocher, Douglas R Mourente, Gabriel |
author_sort |
Betancor, Monica |
title |
Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey |
title_short |
Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey |
title_full |
Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey |
title_fullStr |
Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey |
title_full_unstemmed |
Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey |
title_sort |
lipid metabolism-related gene expression pattern of atlantic bluefin tuna (thunnus thynnus l.) larvae fed on live prey |
publisher |
Springer |
publishDate |
2017 |
url |
http://hdl.handle.net/1893/24381 https://doi.org/10.1007/s10695-016-0305-4 http://dspace.stir.ac.uk/bitstream/1893/24381/1/Betancor_etal_FishPhysiolBiochem_2017.pdf |
genre |
Copepods Rotifer |
genre_facet |
Copepods Rotifer |
op_relation |
Betancor M, Ortega A, de la Gandara F, Tocher DR & Mourente G (2017) Lipid metabolism-related gene expression pattern of Atlantic bluefin tuna (Thunnus thynnus L.) larvae fed on live prey. Fish Physiology and Biochemistry, 43 (2), pp. 493-516. https://doi.org/10.1007/s10695-016-0305-4 http://hdl.handle.net/1893/24381 doi:10.1007/s10695-016-0305-4 27815797 WOS:000399017500016 2-s2.0-84994376829 547287 http://dspace.stir.ac.uk/bitstream/1893/24381/1/Betancor_etal_FishPhysiolBiochem_2017.pdf |
op_rights |
© The Author(s) 2016 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10695-016-0305-4 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
43 |
container_issue |
2 |
container_start_page |
493 |
op_container_end_page |
516 |
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1766243291683094528 |