A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida
Sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. However, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be c...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2016
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Online Access: | http://hdl.handle.net/1893/24317 https://doi.org/10.1016/j.fsi.2016.08.049 http://dspace.stir.ac.uk/bitstream/1893/24317/1/1-s2.0-S1050464816305332-main.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/24317 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Triploid Furunculosis Challenge Vaccination Adhesion Immune response |
spellingShingle |
Triploid Furunculosis Challenge Vaccination Adhesion Immune response Chalmers, Lynn Thompson, Kimberly Taylor, John Black, Sean Migaud, Herve North, Ben Adams, Alexandra A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida |
topic_facet |
Triploid Furunculosis Challenge Vaccination Adhesion Immune response |
description |
Sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. However, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. The aim of this study was to compare the response of triploid Atlantic salmon to a commercial furunculosis vaccine with that of diploid fish, and to assess the vaccine efficacy in the two ploidies through an experimental infection withAeromonas salmonicida. Diploid and triploid Atlantic salmon were injected intraperitoneally with either phosphate buffered saline, liquid paraffin adjuvant or a commercial furunculosis vaccine. Following vaccination, growth, adhesion scores and a variety of assays to assess immune function, such as respiratory burst and antibody response, were measured. Vaccination did not have a significant effect on the weight of either ploidy prior to challenge at 750° days. Adhesion scores were significantly higher in vaccinated fish compared to unvaccinated fish, although no effect of ploidy was observed. Ploidy significantly affected respiratory burst activity following vaccination, however, with triploids exhibiting higher activity than diploids. Combined with lower white blood cell numbers observed in the triploids, it may be that this low cell number is compensated for by increased cellular activity. Ploidy however, did not have a significant effect on complement activity or antibody response, with significantly higher antibody levels detected in all vaccinated fish compared to unvaccinated controls. In addition, both ploidy groups were well protected following challenge with no difference in the relative percentage survival. Based on these results, it appears that ploidy does not affect the severity of adhesions that result post-vaccinate or in the fish's immune response following vaccination, and the furunculosis vaccine performs equally well in both diploid and triploid Atlantic ... |
author2 |
Biotechnology and Biological Sciences Research Council Marine Harvest (Scotland) Ltd Biomar Ltd University of Stirling Institute of Aquaculture Europharma Scotland Ltd PHARMAQ orcid:0000-0002-1271-2991 orcid:0000-0003-4370-7922 orcid:0000-0002-5404-7512 |
format |
Article in Journal/Newspaper |
author |
Chalmers, Lynn Thompson, Kimberly Taylor, John Black, Sean Migaud, Herve North, Ben Adams, Alexandra |
author_facet |
Chalmers, Lynn Thompson, Kimberly Taylor, John Black, Sean Migaud, Herve North, Ben Adams, Alexandra |
author_sort |
Chalmers, Lynn |
title |
A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida |
title_short |
A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida |
title_full |
A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida |
title_fullStr |
A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida |
title_full_unstemmed |
A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida |
title_sort |
comparison of the response of diploid and triploid atlantic salmon (salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with aeromonas salmonicida |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/24317 https://doi.org/10.1016/j.fsi.2016.08.049 http://dspace.stir.ac.uk/bitstream/1893/24317/1/1-s2.0-S1050464816305332-main.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Chalmers L, Thompson K, Taylor J, Black S, Migaud H, North B & Adams A (2016) A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida. Fish and Shellfish Immunology, 57, pp. 301-308. https://doi.org/10.1016/j.fsi.2016.08.049 SALMOTRIP + Optimsation and implementation of sterile triploid salmon in Scotland UoS-BioMar-MH(07-02-2013) BB/M013049/1 http://hdl.handle.net/1893/24317 doi:10.1016/j.fsi.2016.08.049 27569980 WOS:000385327100032 2-s2.0-84984831680 550034 http://dspace.stir.ac.uk/bitstream/1893/24317/1/1-s2.0-S1050464816305332-main.pdf |
op_rights |
© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1016/j.fsi.2016.08.049 |
container_title |
Fish & Shellfish Immunology |
container_volume |
57 |
container_start_page |
301 |
op_container_end_page |
308 |
_version_ |
1766361725564616704 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/24317 2023-05-15T15:31:14+02:00 A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida Chalmers, Lynn Thompson, Kimberly Taylor, John Black, Sean Migaud, Herve North, Ben Adams, Alexandra Biotechnology and Biological Sciences Research Council Marine Harvest (Scotland) Ltd Biomar Ltd University of Stirling Institute of Aquaculture Europharma Scotland Ltd PHARMAQ orcid:0000-0002-1271-2991 orcid:0000-0003-4370-7922 orcid:0000-0002-5404-7512 2016-10 application/pdf http://hdl.handle.net/1893/24317 https://doi.org/10.1016/j.fsi.2016.08.049 http://dspace.stir.ac.uk/bitstream/1893/24317/1/1-s2.0-S1050464816305332-main.pdf en eng Elsevier Chalmers L, Thompson K, Taylor J, Black S, Migaud H, North B & Adams A (2016) A comparison of the response of diploid and triploid Atlantic salmon (Salmo salar) siblings to a commercial furunculosis vaccine and subsequent experimental infection with Aeromonas salmonicida. Fish and Shellfish Immunology, 57, pp. 301-308. https://doi.org/10.1016/j.fsi.2016.08.049 SALMOTRIP + Optimsation and implementation of sterile triploid salmon in Scotland UoS-BioMar-MH(07-02-2013) BB/M013049/1 http://hdl.handle.net/1893/24317 doi:10.1016/j.fsi.2016.08.049 27569980 WOS:000385327100032 2-s2.0-84984831680 550034 http://dspace.stir.ac.uk/bitstream/1893/24317/1/1-s2.0-S1050464816305332-main.pdf © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). http://creativecommons.org/licenses/by/4.0/ CC-BY Triploid Furunculosis Challenge Vaccination Adhesion Immune response Journal Article VoR - Version of Record 2016 ftunivstirling https://doi.org/10.1016/j.fsi.2016.08.049 2022-06-13T18:44:02Z Sterile triploid fish represent a solution to the problems associated with sexual maturation and escapees in aquaculture. However, as disease outbreaks continue to cause significant economic losses to the industry, it is essential that the response of triploids to disease and disease treatments be characterised. The aim of this study was to compare the response of triploid Atlantic salmon to a commercial furunculosis vaccine with that of diploid fish, and to assess the vaccine efficacy in the two ploidies through an experimental infection withAeromonas salmonicida. Diploid and triploid Atlantic salmon were injected intraperitoneally with either phosphate buffered saline, liquid paraffin adjuvant or a commercial furunculosis vaccine. Following vaccination, growth, adhesion scores and a variety of assays to assess immune function, such as respiratory burst and antibody response, were measured. Vaccination did not have a significant effect on the weight of either ploidy prior to challenge at 750° days. Adhesion scores were significantly higher in vaccinated fish compared to unvaccinated fish, although no effect of ploidy was observed. Ploidy significantly affected respiratory burst activity following vaccination, however, with triploids exhibiting higher activity than diploids. Combined with lower white blood cell numbers observed in the triploids, it may be that this low cell number is compensated for by increased cellular activity. Ploidy however, did not have a significant effect on complement activity or antibody response, with significantly higher antibody levels detected in all vaccinated fish compared to unvaccinated controls. In addition, both ploidy groups were well protected following challenge with no difference in the relative percentage survival. Based on these results, it appears that ploidy does not affect the severity of adhesions that result post-vaccinate or in the fish's immune response following vaccination, and the furunculosis vaccine performs equally well in both diploid and triploid Atlantic ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Fish & Shellfish Immunology 57 301 308 |