Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar)
Legumes such as soybean, peas and lupin have attracted considerable interest as potential sources of protein to replace finite and limiting supplies of marine fishmeal (FM) as major ingredients for aquafeeds. In this respect, faba beans (Vicia faba) represent awidespread and relatively unexploited l...
Published in: | Aquaculture |
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Main Authors: | , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2016
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Online Access: | http://hdl.handle.net/1893/24189 https://doi.org/10.1016/j.aquaculture.2016.09.008 http://dspace.stir.ac.uk/bitstream/1893/24189/1/Final%20submitted%20MS.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/24189 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon Faba bean Protein isolate Wet process Growth performance Composition Liver Pyloric caeca Distal intestine Histology Transcriptomics |
spellingShingle |
Atlantic salmon Faba bean Protein isolate Wet process Growth performance Composition Liver Pyloric caeca Distal intestine Histology Transcriptomics De Santis, Christian Martin, Samuel A M Dehler, Carola E Iannetta, Pietro P M Leeming, Daniel Tocher, Douglas R Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) |
topic_facet |
Atlantic salmon Faba bean Protein isolate Wet process Growth performance Composition Liver Pyloric caeca Distal intestine Histology Transcriptomics |
description |
Legumes such as soybean, peas and lupin have attracted considerable interest as potential sources of protein to replace finite and limiting supplies of marine fishmeal (FM) as major ingredients for aquafeeds. In this respect, faba beans (Vicia faba) represent awidespread and relatively unexploited legumecrop in Europewith potentially favourable characteristics. However, for carnivorous species such as Atlantic salmon (Salmo salar), protein levels in legumes are generally too lowand require to be concentrated to be direct replacements for FM. Previouslywe showed that a faba bean protein concentrate, produced by air classification, containing 55% protein could partially replace FM and/or soy protein concentrate (SPC) in feeds for parr and post-smolt salmon. In the present study, a faba bean protein isolate (BPI), produced by a wet process, with almost ~80% crude proteinwas investigated in feeds for Atlantic salmon in seawater. Four dietary treatments were tested including one with high inclusion of FM (400 g kg−1) and three with low FM (216 g kg−1) and increasing inclusions of BPI (0, 70 and 140 g kg−1) substituting for SPC (236, 125 and 45 g kg−1). Growth performance in fish was unaffected with the lower level of dietary BPI, butwas reduced in fish fed the higher level, mainly due to feed intake being reduced initially. Histological analysis of the distal intestine showed inflammation in fish fed both diets containing BPI, but especially at 140 g kg−1. The high dietary level of BPI affected the transcriptome of pyloric caeca with almost 2000 differentially expressed genes (DEG) compared to fish fed FM, whereas fish fed SPC or the lower level of BPI showed no DEG compared to fish fed FM. In contrast, the liver transcriptomewas generally affected similarly by both BPI and SPC. The combined data suggested that the BPI utilised contained a factor that was detrimental above a certain threshold and, although this factor could be an artefact of the protein isolation method, an effect of a known ANF could not be excluded ... |
author2 |
Technology Strategy Board Institute of Aquaculture University of Aberdeen The James Hutton Institute BioMar Ltd orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
De Santis, Christian Martin, Samuel A M Dehler, Carola E Iannetta, Pietro P M Leeming, Daniel Tocher, Douglas R |
author_facet |
De Santis, Christian Martin, Samuel A M Dehler, Carola E Iannetta, Pietro P M Leeming, Daniel Tocher, Douglas R |
author_sort |
De Santis, Christian |
title |
Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) |
title_short |
Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) |
title_full |
Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) |
title_fullStr |
Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) |
title_sort |
influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt atlantic salmon (salmo salar) |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/24189 https://doi.org/10.1016/j.aquaculture.2016.09.008 http://dspace.stir.ac.uk/bitstream/1893/24189/1/Final%20submitted%20MS.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
De Santis C, Martin SAM, Dehler CE, Iannetta PPM, Leeming D & Tocher DR (2016) Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar). Aquaculture, 465, pp. 124-133. https://doi.org/10.1016/j.aquaculture.2016.09.008 Development of a Protein-Rich and Starch-Rich Fractions from Faba Beans for Salmon and Terrestrial Animal Production, TS/J002968/1 http://hdl.handle.net/1893/24189 doi:10.1016/j.aquaculture.2016.09.008 WOS:000385372200018 2-s2.0-84986600136 549813 http://dspace.stir.ac.uk/bitstream/1893/24189/1/Final%20submitted%20MS.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: De Santis C, Martin SAM, Dehler C, Iannetta P, Leeming D & Tocher DR (2016) Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar)., Aquaculture, 465, pp. 124-133. DOI:10.1016/j.aquaculture.2016.09.008 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ [Final submitted MS.pdf] Publisher requires embargo of 12 months after formal publication. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.aquaculture.2016.09.008 |
container_title |
Aquaculture |
container_volume |
465 |
container_start_page |
124 |
op_container_end_page |
133 |
_version_ |
1766361354999955456 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/24189 2023-05-15T15:30:53+02:00 Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar) De Santis, Christian Martin, Samuel A M Dehler, Carola E Iannetta, Pietro P M Leeming, Daniel Tocher, Douglas R Technology Strategy Board Institute of Aquaculture University of Aberdeen The James Hutton Institute BioMar Ltd orcid:0000-0002-8603-9410 2016-12 application/pdf http://hdl.handle.net/1893/24189 https://doi.org/10.1016/j.aquaculture.2016.09.008 http://dspace.stir.ac.uk/bitstream/1893/24189/1/Final%20submitted%20MS.pdf en eng Elsevier De Santis C, Martin SAM, Dehler CE, Iannetta PPM, Leeming D & Tocher DR (2016) Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar). Aquaculture, 465, pp. 124-133. https://doi.org/10.1016/j.aquaculture.2016.09.008 Development of a Protein-Rich and Starch-Rich Fractions from Faba Beans for Salmon and Terrestrial Animal Production, TS/J002968/1 http://hdl.handle.net/1893/24189 doi:10.1016/j.aquaculture.2016.09.008 WOS:000385372200018 2-s2.0-84986600136 549813 http://dspace.stir.ac.uk/bitstream/1893/24189/1/Final%20submitted%20MS.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: De Santis C, Martin SAM, Dehler C, Iannetta P, Leeming D & Tocher DR (2016) Influence of dietary inclusion of a wet processed faba bean protein isolate on post-smolt Atlantic salmon (Salmo salar)., Aquaculture, 465, pp. 124-133. DOI:10.1016/j.aquaculture.2016.09.008 © 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ [Final submitted MS.pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND Atlantic salmon Faba bean Protein isolate Wet process Growth performance Composition Liver Pyloric caeca Distal intestine Histology Transcriptomics Journal Article AM - Accepted Manuscript 2016 ftunivstirling https://doi.org/10.1016/j.aquaculture.2016.09.008 2022-06-13T18:45:10Z Legumes such as soybean, peas and lupin have attracted considerable interest as potential sources of protein to replace finite and limiting supplies of marine fishmeal (FM) as major ingredients for aquafeeds. In this respect, faba beans (Vicia faba) represent awidespread and relatively unexploited legumecrop in Europewith potentially favourable characteristics. However, for carnivorous species such as Atlantic salmon (Salmo salar), protein levels in legumes are generally too lowand require to be concentrated to be direct replacements for FM. Previouslywe showed that a faba bean protein concentrate, produced by air classification, containing 55% protein could partially replace FM and/or soy protein concentrate (SPC) in feeds for parr and post-smolt salmon. In the present study, a faba bean protein isolate (BPI), produced by a wet process, with almost ~80% crude proteinwas investigated in feeds for Atlantic salmon in seawater. Four dietary treatments were tested including one with high inclusion of FM (400 g kg−1) and three with low FM (216 g kg−1) and increasing inclusions of BPI (0, 70 and 140 g kg−1) substituting for SPC (236, 125 and 45 g kg−1). Growth performance in fish was unaffected with the lower level of dietary BPI, butwas reduced in fish fed the higher level, mainly due to feed intake being reduced initially. Histological analysis of the distal intestine showed inflammation in fish fed both diets containing BPI, but especially at 140 g kg−1. The high dietary level of BPI affected the transcriptome of pyloric caeca with almost 2000 differentially expressed genes (DEG) compared to fish fed FM, whereas fish fed SPC or the lower level of BPI showed no DEG compared to fish fed FM. In contrast, the liver transcriptomewas generally affected similarly by both BPI and SPC. The combined data suggested that the BPI utilised contained a factor that was detrimental above a certain threshold and, although this factor could be an artefact of the protein isolation method, an effect of a known ANF could not be excluded ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Aquaculture 465 124 133 |