Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology
Infectious salmon anaemia virus (ISAV) is an orthomyxovirus that has had a significant economic impact on Atlantic salmon farming in Europe, North America and Chile. Monoclonal antibodies (mAbs) were developed against Segment 3 (encoding the viral nucleoprotein, NP) of the virus. Six of the mAbs wer...
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Online Access: | http://hdl.handle.net/1893/23960 https://doi.org/10.1371/journal.pone.0159155 http://dspace.stir.ac.uk/bitstream/1893/23960/1/journal.pone.0159155.PDF |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/23960 2023-05-15T15:31:51+02:00 Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology Hoare, Rowena Thompson, Kim D Herath, Tharangani Collet, Bertrand Bron, James Adams, Alexandra Medical Research Council Institute of Aquaculture The Moredun Research Institute Harper Adams University Scottish Government - Enterprise, Environment & Digital - Marine Scotland orcid:0000-0002-9298-4275 orcid:0000-0003-3544-0519 2016-07-19 application/pdf http://hdl.handle.net/1893/23960 https://doi.org/10.1371/journal.pone.0159155 http://dspace.stir.ac.uk/bitstream/1893/23960/1/journal.pone.0159155.PDF en eng Public Library of Science Hoare R, Thompson KD, Herath T, Collet B, Bron J & Adams A (2016) Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology. PLoS ONE, 11 (7), Art. No.: e0159155. https://doi.org/10.1371/journal.pone.0159155 Development of a non lethal sampling method to monitor immune response and disease progression in salmonid fish n/a e0159155 http://hdl.handle.net/1893/23960 doi:10.1371/journal.pone.0159155 27434377 WOS:000380169600036 2-s2.0-84979293986 553695 http://dspace.stir.ac.uk/bitstream/1893/23960/1/journal.pone.0159155.PDF © 2016 Hoare et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ CC-BY Journal Article VoR - Version of Record 2016 ftunivstirling https://doi.org/10.1371/journal.pone.0159155 2022-06-13T18:43:37Z Infectious salmon anaemia virus (ISAV) is an orthomyxovirus that has had a significant economic impact on Atlantic salmon farming in Europe, North America and Chile. Monoclonal antibodies (mAbs) were developed against Segment 3 (encoding the viral nucleoprotein, NP) of the virus. Six of the mAbs were shown to be specific to ISAV and recognised all isolates from Scotland, Norway and Canada. They reacted with ISAV in enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody technique (IFAT) and western blotting. They were also used to develop a novel detection method based on Luminex (Bio-Plex) bead-based flow cytometric technology for the detection of ISAV in the plasma of Atlantic salmon (Salmo salarL.) smolts experimentally infected with ISAV. Fish were challenged by intraperitoneal (i.p.) injection of virus at 50% Tissue Culture Infective Dose (TCID50) = 2.8 x106per animal. Virus present in plasma of infected fish, collected at 0, 4, 8, 12, 16, 21 and 28 days post infection using a non-lethal sampling method (n = 12 at each time point), was quantified using the optimised Bio-Plex assay. The results obtained with this assay were compared with absolute quantification of the virus by RT-qPCR using SYBR Green I and TaqMan chemistries. The Bio-Plex assay developed using the NP mAbs appears to be a rapid, sensitive method for detecting and quantifying ISAV in small volumes of fish plasma and has the potential to be multiplexed for the detection of other fish pathogens (e.g. during co-infections). To our knowledge this is the first report of the use of Luminex (Bio-Plex) technology for the detection of a fish pathogen. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Canada Norway PLOS ONE 11 7 e0159155 |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
description |
Infectious salmon anaemia virus (ISAV) is an orthomyxovirus that has had a significant economic impact on Atlantic salmon farming in Europe, North America and Chile. Monoclonal antibodies (mAbs) were developed against Segment 3 (encoding the viral nucleoprotein, NP) of the virus. Six of the mAbs were shown to be specific to ISAV and recognised all isolates from Scotland, Norway and Canada. They reacted with ISAV in enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody technique (IFAT) and western blotting. They were also used to develop a novel detection method based on Luminex (Bio-Plex) bead-based flow cytometric technology for the detection of ISAV in the plasma of Atlantic salmon (Salmo salarL.) smolts experimentally infected with ISAV. Fish were challenged by intraperitoneal (i.p.) injection of virus at 50% Tissue Culture Infective Dose (TCID50) = 2.8 x106per animal. Virus present in plasma of infected fish, collected at 0, 4, 8, 12, 16, 21 and 28 days post infection using a non-lethal sampling method (n = 12 at each time point), was quantified using the optimised Bio-Plex assay. The results obtained with this assay were compared with absolute quantification of the virus by RT-qPCR using SYBR Green I and TaqMan chemistries. The Bio-Plex assay developed using the NP mAbs appears to be a rapid, sensitive method for detecting and quantifying ISAV in small volumes of fish plasma and has the potential to be multiplexed for the detection of other fish pathogens (e.g. during co-infections). To our knowledge this is the first report of the use of Luminex (Bio-Plex) technology for the detection of a fish pathogen. |
author2 |
Medical Research Council Institute of Aquaculture The Moredun Research Institute Harper Adams University Scottish Government - Enterprise, Environment & Digital - Marine Scotland orcid:0000-0002-9298-4275 orcid:0000-0003-3544-0519 |
format |
Article in Journal/Newspaper |
author |
Hoare, Rowena Thompson, Kim D Herath, Tharangani Collet, Bertrand Bron, James Adams, Alexandra |
spellingShingle |
Hoare, Rowena Thompson, Kim D Herath, Tharangani Collet, Bertrand Bron, James Adams, Alexandra Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology |
author_facet |
Hoare, Rowena Thompson, Kim D Herath, Tharangani Collet, Bertrand Bron, James Adams, Alexandra |
author_sort |
Hoare, Rowena |
title |
Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology |
title_short |
Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology |
title_full |
Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology |
title_fullStr |
Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology |
title_full_unstemmed |
Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology |
title_sort |
development, characterisation and application of monoclonal antibodies for the detection and quantification of infectious salmon anaemia virus in plasma samples using luminex bead array technology |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/23960 https://doi.org/10.1371/journal.pone.0159155 http://dspace.stir.ac.uk/bitstream/1893/23960/1/journal.pone.0159155.PDF |
geographic |
Canada Norway |
geographic_facet |
Canada Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Hoare R, Thompson KD, Herath T, Collet B, Bron J & Adams A (2016) Development, Characterisation and Application of Monoclonal Antibodies for the Detection and Quantification of Infectious Salmon Anaemia Virus in Plasma Samples Using Luminex Bead Array Technology. PLoS ONE, 11 (7), Art. No.: e0159155. https://doi.org/10.1371/journal.pone.0159155 Development of a non lethal sampling method to monitor immune response and disease progression in salmonid fish n/a e0159155 http://hdl.handle.net/1893/23960 doi:10.1371/journal.pone.0159155 27434377 WOS:000380169600036 2-s2.0-84979293986 553695 http://dspace.stir.ac.uk/bitstream/1893/23960/1/journal.pone.0159155.PDF |
op_rights |
© 2016 Hoare et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0159155 |
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