Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.)
Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid...
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Online Access: | http://hdl.handle.net/1893/23916 https://doi.org/10.1371/journal.pone.0159934 http://dspace.stir.ac.uk/bitstream/1893/23916/1/journal.pone.0159934.PDF |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/23916 2023-05-15T15:31:51+02:00 Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) Betancor, Monica Sprague, Matthew Sayanova, Olga Usher, Sarah Metochis, Christoforos Campbell, Patrick Napier, Johnathan A Tocher, Douglas R Biomar Ltd Institute of Aquaculture Rothamsted Research BioMar U.K. orcid:0000-0003-1626-7458 orcid:0000-0002-0723-2387 orcid:0000-0002-3299-0630 orcid:0000-0002-8603-9410 2016-07-25 application/pdf http://hdl.handle.net/1893/23916 https://doi.org/10.1371/journal.pone.0159934 http://dspace.stir.ac.uk/bitstream/1893/23916/1/journal.pone.0159934.PDF en eng Public Library of Science Betancor M, Sprague M, Sayanova O, Usher S, Metochis C, Campbell P, Napier JA & Tocher DR (2016) Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.). PLoS ONE, 11 (7), Art. No.: e0159934. https://doi.org/10.1371/journal.pone.0159934 Evaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquaculture BB/J001252/1 e0159934 http://hdl.handle.net/1893/23916 doi:10.1371/journal.pone.0159934 27454884 WOS:000381515200058 2-s2.0-84980426599 555161 http://dspace.stir.ac.uk/bitstream/1893/23916/1/journal.pone.0159934.PDF © 2016 Betancor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ CC-BY Journal Article VoR - Version of Record 2016 ftunivstirling https://doi.org/10.1371/journal.pone.0159934 2022-06-13T18:44:25Z Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, representing a potential option to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil with around 15 % total n-3 LC-PUFA to potentially substitute for fish oil in salmon feeds. Post-smolt Atlantic salmon (Salmo salar) were fed for 11-weeks with one of three experimental diets containing either fish oil (FO), wild-type Camelina oil (WCO) or transgenic Camelina oil (DCO) as added lipid source to evaluate fish performance, nutrient digestibility, tissue n-3 LC-PUFA, and metabolic impact determined by liver transcriptome analysis. The DCO diet did not affect any of the performance or health parameters studied and enhanced apparent digestibility of EPA and DHA compared to the WCO diet. The level of total n-3 LC-PUFA was higher in all the tissues of DCO-fed fish than in WCO-fed fish with levels in liver similar to those in fish fed FO. Endogenous LC-PUFA biosynthetic activity was observed in fish fed both the Camelina oil diets as indicated by the liver transcriptome and levels of intermediate metabolites such as docosapentaenoic acid, with data suggesting that the dietary combination of EPA and DHA inhibited desaturation and elongation activities. Expression of genes involved in phospholipid and triacylglycerol metabolism followed a similar pattern in fish fed DCO and WCO despite the difference in n-3 LC-PUFA contents. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository PLOS ONE 11 7 e0159934 |
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Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
description |
Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, representing a potential option to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil with around 15 % total n-3 LC-PUFA to potentially substitute for fish oil in salmon feeds. Post-smolt Atlantic salmon (Salmo salar) were fed for 11-weeks with one of three experimental diets containing either fish oil (FO), wild-type Camelina oil (WCO) or transgenic Camelina oil (DCO) as added lipid source to evaluate fish performance, nutrient digestibility, tissue n-3 LC-PUFA, and metabolic impact determined by liver transcriptome analysis. The DCO diet did not affect any of the performance or health parameters studied and enhanced apparent digestibility of EPA and DHA compared to the WCO diet. The level of total n-3 LC-PUFA was higher in all the tissues of DCO-fed fish than in WCO-fed fish with levels in liver similar to those in fish fed FO. Endogenous LC-PUFA biosynthetic activity was observed in fish fed both the Camelina oil diets as indicated by the liver transcriptome and levels of intermediate metabolites such as docosapentaenoic acid, with data suggesting that the dietary combination of EPA and DHA inhibited desaturation and elongation activities. Expression of genes involved in phospholipid and triacylglycerol metabolism followed a similar pattern in fish fed DCO and WCO despite the difference in n-3 LC-PUFA contents. |
author2 |
Biomar Ltd Institute of Aquaculture Rothamsted Research BioMar U.K. orcid:0000-0003-1626-7458 orcid:0000-0002-0723-2387 orcid:0000-0002-3299-0630 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Betancor, Monica Sprague, Matthew Sayanova, Olga Usher, Sarah Metochis, Christoforos Campbell, Patrick Napier, Johnathan A Tocher, Douglas R |
spellingShingle |
Betancor, Monica Sprague, Matthew Sayanova, Olga Usher, Sarah Metochis, Christoforos Campbell, Patrick Napier, Johnathan A Tocher, Douglas R Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) |
author_facet |
Betancor, Monica Sprague, Matthew Sayanova, Olga Usher, Sarah Metochis, Christoforos Campbell, Patrick Napier, Johnathan A Tocher, Douglas R |
author_sort |
Betancor, Monica |
title |
Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) |
title_short |
Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) |
title_full |
Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) |
title_fullStr |
Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.) |
title_sort |
nutritional evaluation of an epa-dha oil from transgenic camelina sativa in feeds for post-smolt atlantic salmon (salmo salar l.) |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/23916 https://doi.org/10.1371/journal.pone.0159934 http://dspace.stir.ac.uk/bitstream/1893/23916/1/journal.pone.0159934.PDF |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Betancor M, Sprague M, Sayanova O, Usher S, Metochis C, Campbell P, Napier JA & Tocher DR (2016) Nutritional evaluation of an EPA-DHA oil from transgenic Camelina sativa in feeds for post-smolt Atlantic salmon (Salmo salar L.). PLoS ONE, 11 (7), Art. No.: e0159934. https://doi.org/10.1371/journal.pone.0159934 Evaluating novel plant oilseeds enriched in omega-3 long-chain polyunsaturated fatty acids to support sustainable development of aquaculture BB/J001252/1 e0159934 http://hdl.handle.net/1893/23916 doi:10.1371/journal.pone.0159934 27454884 WOS:000381515200058 2-s2.0-84980426599 555161 http://dspace.stir.ac.uk/bitstream/1893/23916/1/journal.pone.0159934.PDF |
op_rights |
© 2016 Betancor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0159934 |
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PLOS ONE |
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11 |
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e0159934 |
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