Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication
Fatty acyl elongase 5 (elovl5) is a critical enzyme in the vertebrate biosynthetic pathway which produces the physiologically essential long chain polyunsaturated fatty acids (LC-PUFA), docosahexenoic acid (DHA) and eicosapentenoic acid (EPA) from 18 carbon fatty acids precursors. In contrast to mos...
Published in: | Gene |
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Main Authors: | , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2016
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Online Access: | http://hdl.handle.net/1893/23504 https://doi.org/10.1016/j.gene.2016.06.056 http://dspace.stir.ac.uk/bitstream/1893/23504/1/M%20Leaver%20Gene%20300516%20%282%29.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/23504 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon DNA transposon homeologous genes neofunctionalisation transposable elements whole-genome duplication fatty acid biosynthesis |
spellingShingle |
Atlantic salmon DNA transposon homeologous genes neofunctionalisation transposable elements whole-genome duplication fatty acid biosynthesis Carmona-Antonanzas, Greta Zheng, Xiaozhong Tocher, Douglas R Leaver, Michael Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
topic_facet |
Atlantic salmon DNA transposon homeologous genes neofunctionalisation transposable elements whole-genome duplication fatty acid biosynthesis |
description |
Fatty acyl elongase 5 (elovl5) is a critical enzyme in the vertebrate biosynthetic pathway which produces the physiologically essential long chain polyunsaturated fatty acids (LC-PUFA), docosahexenoic acid (DHA) and eicosapentenoic acid (EPA) from 18 carbon fatty acids precursors. In contrast to most other vertebrates, Atlantic salmon possess two copies of elovl5 (elovl5a and elovl5b) as a result of a whole genome duplication (WGD) which occurred at the base of the salmonid lineage. WGDs have had a major influence on vertebrate evolution, providing extra genetic material, enabling neofunctionalization to accelerate adaptation and speciation. However, little is known about the mechanisms by which such duplicated homeologous genes diverge. Here we show that homeologous Atlantic salmon elovl5a and elovl5b genes have been asymmetrically colonised by transposon-like elements. Identical locations and identities of insertions are also present in the rainbow trout duplicate elovl5 genes, but not in the nearest extant representative preduplicated teleost, the northern pike. Both elovl5 salmon duplicates possessed conserved regulatory elements that promoted Srebp1- and Srebp2-dependent transcription, and differences in the magnitude of Srebp response between promoters could be attributed to a tandem duplication of SRE and NF-Y cofactor binding sites in elovl5b. Furthermore, an insertion in the promoter region of elovl5a confers responsiveness to Lxr/Rxr transcriptional activation. Our results indicate that most, but not all transposon mobilisation into elovl5 genes occurred after the split from the common ancestor of pike and salmon, but before more recent salmonid speciations, and that divergence of elovl5 regulatory regions have enabled neofuntionalization by promoting differential expression of these homeologous genes. |
author2 |
Institute of Aquaculture orcid:0000-0002-8603-9410 orcid:0000-0002-3155-0844 |
format |
Article in Journal/Newspaper |
author |
Carmona-Antonanzas, Greta Zheng, Xiaozhong Tocher, Douglas R Leaver, Michael |
author_facet |
Carmona-Antonanzas, Greta Zheng, Xiaozhong Tocher, Douglas R Leaver, Michael |
author_sort |
Carmona-Antonanzas, Greta |
title |
Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
title_short |
Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
title_full |
Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
title_fullStr |
Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
title_full_unstemmed |
Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
title_sort |
regulatory divergence of homeologous atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication |
publisher |
Elsevier |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/23504 https://doi.org/10.1016/j.gene.2016.06.056 http://dspace.stir.ac.uk/bitstream/1893/23504/1/M%20Leaver%20Gene%20300516%20%282%29.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Carmona-Antonanzas G, Zheng X, Tocher DR & Leaver M (2016) Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication. Gene, 591 (1), pp. 34-42. https://doi.org/10.1016/j.gene.2016.06.056 http://hdl.handle.net/1893/23504 doi:10.1016/j.gene.2016.06.056 27374149 WOS:000382409400006 2-s2.0-84985945894 561595 http://dspace.stir.ac.uk/bitstream/1893/23504/1/M%20Leaver%20Gene%20300516%20%282%29.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Carmona-Antonanzas G, Zheng X, Tocher DR & Leaver M (2016) Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication, Gene, 591 (1), pp. 34-42. DOI:10.1016/j.gene.2016.06.056 © 2016, Elsevier. Licensed under the Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2017-07-01 [M Leaver Gene 300516 (2).pdf] Publisher requires embargo of 12 months after formal publication. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.gene.2016.06.056 |
container_title |
Gene |
container_volume |
591 |
container_issue |
1 |
container_start_page |
34 |
op_container_end_page |
42 |
_version_ |
1766360967897153536 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/23504 2023-05-15T15:30:31+02:00 Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication Carmona-Antonanzas, Greta Zheng, Xiaozhong Tocher, Douglas R Leaver, Michael Institute of Aquaculture orcid:0000-0002-8603-9410 orcid:0000-0002-3155-0844 2016-10-10 application/pdf http://hdl.handle.net/1893/23504 https://doi.org/10.1016/j.gene.2016.06.056 http://dspace.stir.ac.uk/bitstream/1893/23504/1/M%20Leaver%20Gene%20300516%20%282%29.pdf en eng Elsevier Carmona-Antonanzas G, Zheng X, Tocher DR & Leaver M (2016) Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication. Gene, 591 (1), pp. 34-42. https://doi.org/10.1016/j.gene.2016.06.056 http://hdl.handle.net/1893/23504 doi:10.1016/j.gene.2016.06.056 27374149 WOS:000382409400006 2-s2.0-84985945894 561595 http://dspace.stir.ac.uk/bitstream/1893/23504/1/M%20Leaver%20Gene%20300516%20%282%29.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Carmona-Antonanzas G, Zheng X, Tocher DR & Leaver M (2016) Regulatory divergence of homeologous Atlantic salmon elovl5 genes following the salmonid-specific whole genome duplication, Gene, 591 (1), pp. 34-42. DOI:10.1016/j.gene.2016.06.056 © 2016, Elsevier. Licensed under the Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ 2017-07-01 [M Leaver Gene 300516 (2).pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND Atlantic salmon DNA transposon homeologous genes neofunctionalisation transposable elements whole-genome duplication fatty acid biosynthesis Journal Article AM - Accepted Manuscript 2016 ftunivstirling https://doi.org/10.1016/j.gene.2016.06.056 2022-06-13T18:44:24Z Fatty acyl elongase 5 (elovl5) is a critical enzyme in the vertebrate biosynthetic pathway which produces the physiologically essential long chain polyunsaturated fatty acids (LC-PUFA), docosahexenoic acid (DHA) and eicosapentenoic acid (EPA) from 18 carbon fatty acids precursors. In contrast to most other vertebrates, Atlantic salmon possess two copies of elovl5 (elovl5a and elovl5b) as a result of a whole genome duplication (WGD) which occurred at the base of the salmonid lineage. WGDs have had a major influence on vertebrate evolution, providing extra genetic material, enabling neofunctionalization to accelerate adaptation and speciation. However, little is known about the mechanisms by which such duplicated homeologous genes diverge. Here we show that homeologous Atlantic salmon elovl5a and elovl5b genes have been asymmetrically colonised by transposon-like elements. Identical locations and identities of insertions are also present in the rainbow trout duplicate elovl5 genes, but not in the nearest extant representative preduplicated teleost, the northern pike. Both elovl5 salmon duplicates possessed conserved regulatory elements that promoted Srebp1- and Srebp2-dependent transcription, and differences in the magnitude of Srebp response between promoters could be attributed to a tandem duplication of SRE and NF-Y cofactor binding sites in elovl5b. Furthermore, an insertion in the promoter region of elovl5a confers responsiveness to Lxr/Rxr transcriptional activation. Our results indicate that most, but not all transposon mobilisation into elovl5 genes occurred after the split from the common ancestor of pike and salmon, but before more recent salmonid speciations, and that divergence of elovl5 regulatory regions have enabled neofuntionalization by promoting differential expression of these homeologous genes. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Gene 591 1 34 42 |