Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner
Daily variation in the absorption, metabolism and excretion of toxic substances will ultimately determine the actual concentration to which the cells and tissues are exposed. In aquaculture, Atlantic salmon (Salmo salar) can be frequently exposed to hydrogen peroxide (H2O2) to treat topical skin and...
Published in: | Chronobiology International |
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Taylor and Francis
2016
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ftunivstirling:oai:dspace.stir.ac.uk:1893/23159 2023-05-15T15:31:14+02:00 Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner Vera, LM Migaud, Herve Institute of Aquaculture orcid:0000-0003-0999-055X orcid:0000-0002-5404-7512 2016 application/pdf http://hdl.handle.net/1893/23159 https://doi.org/10.3109/07420528.2015.1131164 http://dspace.stir.ac.uk/bitstream/1893/23159/1/Hydrogen_peroxide_chronotoxicity_in_salmon.pdf en eng Taylor and Francis Vera L & Migaud H (2016) Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner. Chronobiology International, 33 (5), pp. 530-542. https://doi.org/10.3109/07420528.2015.1131164 http://hdl.handle.net/1893/23159 doi:10.3109/07420528.2015.1131164 27058450 WOS:000379779000006 2-s2.0-84964055112 572604 http://dspace.stir.ac.uk/bitstream/1893/23159/1/Hydrogen_peroxide_chronotoxicity_in_salmon.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Chronobiology International on 08 April 2016, available online: http://www.tandfonline.com/10.3109/07420528.2015.1131164 2017-04-09 [Hydrogen_peroxide_chronotoxicity_in_salmon.pdf] Publisher requires embargo of 12 months after formal publication. Atlantic salmon chronotherapy chronotoxicity daily rhythms hydrogen peroxide oxidative stress stress response Journal Article AM - Accepted Manuscript 2016 ftunivstirling https://doi.org/10.3109/07420528.2015.1131164 2022-06-13T18:43:45Z Daily variation in the absorption, metabolism and excretion of toxic substances will ultimately determine the actual concentration to which the cells and tissues are exposed. In aquaculture, Atlantic salmon (Salmo salar) can be frequently exposed to hydrogen peroxide (H2O2) to treat topical skin and gill infections, particularly in relation to parasitic infections (e.g. sea liceLepeophtheirus salmonisand amoebic gill disease caused byNeoparamoeba perurans). It is well accepted that the time of administration influences pharmacodynamics and pharmacokinetics of drugs which in turn affects their efficacy and toxicity. Consequently, a better understanding of drug side effects as a function of time of day exposure would help to improve treatment efficacy and fish welfare. To this end, salmon were exposed to H2O2(1500 mg/L) for 20 min at six different times of the day during a 24-h cycle and we investigated the time-dependent effects of exposure on physiological stress (glucose, lactate and cortisol) and antioxidant enzyme expression (gpx1, cat, Mn-sodandhsp70) in liver and gills. In addition, at each sampling point, 8 control fish were also sampled. Our results revealed that the time of administration of H2O2caused significant differences in the induction of both physiological and oxidative stress responses. Glucose and lactate were higher in the treated fish during daytime whereas cortisol levels appeared to be systematically increased (>1000 ng/mL) after H2O2treatment irrespective of exposure time, although differences with control levels were higher during the day. In liver, gene expression of antioxidant enzymes displayed daily rhythmicity in both treated and control groups and showed higher mRNA expression levels in salmon treated with H2O2at ZT6 (6 h after lights onset). In gills, rhythmic expression was only found forgpx1in the control fish and forhsp70andMn-sodin the treated groups. However, in the treated salmon, higher gene expression levels of all the investigated enzymes were also observed at ZT6-10. ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Chronobiology International 33 5 530 542 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon chronotherapy chronotoxicity daily rhythms hydrogen peroxide oxidative stress stress response |
spellingShingle |
Atlantic salmon chronotherapy chronotoxicity daily rhythms hydrogen peroxide oxidative stress stress response Vera, LM Migaud, Herve Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner |
topic_facet |
Atlantic salmon chronotherapy chronotoxicity daily rhythms hydrogen peroxide oxidative stress stress response |
description |
Daily variation in the absorption, metabolism and excretion of toxic substances will ultimately determine the actual concentration to which the cells and tissues are exposed. In aquaculture, Atlantic salmon (Salmo salar) can be frequently exposed to hydrogen peroxide (H2O2) to treat topical skin and gill infections, particularly in relation to parasitic infections (e.g. sea liceLepeophtheirus salmonisand amoebic gill disease caused byNeoparamoeba perurans). It is well accepted that the time of administration influences pharmacodynamics and pharmacokinetics of drugs which in turn affects their efficacy and toxicity. Consequently, a better understanding of drug side effects as a function of time of day exposure would help to improve treatment efficacy and fish welfare. To this end, salmon were exposed to H2O2(1500 mg/L) for 20 min at six different times of the day during a 24-h cycle and we investigated the time-dependent effects of exposure on physiological stress (glucose, lactate and cortisol) and antioxidant enzyme expression (gpx1, cat, Mn-sodandhsp70) in liver and gills. In addition, at each sampling point, 8 control fish were also sampled. Our results revealed that the time of administration of H2O2caused significant differences in the induction of both physiological and oxidative stress responses. Glucose and lactate were higher in the treated fish during daytime whereas cortisol levels appeared to be systematically increased (>1000 ng/mL) after H2O2treatment irrespective of exposure time, although differences with control levels were higher during the day. In liver, gene expression of antioxidant enzymes displayed daily rhythmicity in both treated and control groups and showed higher mRNA expression levels in salmon treated with H2O2at ZT6 (6 h after lights onset). In gills, rhythmic expression was only found forgpx1in the control fish and forhsp70andMn-sodin the treated groups. However, in the treated salmon, higher gene expression levels of all the investigated enzymes were also observed at ZT6-10. ... |
author2 |
Institute of Aquaculture orcid:0000-0003-0999-055X orcid:0000-0002-5404-7512 |
format |
Article in Journal/Newspaper |
author |
Vera, LM Migaud, Herve |
author_facet |
Vera, LM Migaud, Herve |
author_sort |
Vera, LM |
title |
Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner |
title_short |
Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner |
title_full |
Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner |
title_fullStr |
Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner |
title_full_unstemmed |
Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner |
title_sort |
hydrogen peroxide treatment in atlantic salmon induces stress and detoxification response in a daily manner |
publisher |
Taylor and Francis |
publishDate |
2016 |
url |
http://hdl.handle.net/1893/23159 https://doi.org/10.3109/07420528.2015.1131164 http://dspace.stir.ac.uk/bitstream/1893/23159/1/Hydrogen_peroxide_chronotoxicity_in_salmon.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Vera L & Migaud H (2016) Hydrogen peroxide treatment in Atlantic salmon induces stress and detoxification response in a daily manner. Chronobiology International, 33 (5), pp. 530-542. https://doi.org/10.3109/07420528.2015.1131164 http://hdl.handle.net/1893/23159 doi:10.3109/07420528.2015.1131164 27058450 WOS:000379779000006 2-s2.0-84964055112 572604 http://dspace.stir.ac.uk/bitstream/1893/23159/1/Hydrogen_peroxide_chronotoxicity_in_salmon.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Chronobiology International on 08 April 2016, available online: http://www.tandfonline.com/10.3109/07420528.2015.1131164 2017-04-09 [Hydrogen_peroxide_chronotoxicity_in_salmon.pdf] Publisher requires embargo of 12 months after formal publication. |
op_doi |
https://doi.org/10.3109/07420528.2015.1131164 |
container_title |
Chronobiology International |
container_volume |
33 |
container_issue |
5 |
container_start_page |
530 |
op_container_end_page |
542 |
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1766361727859949568 |