Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry
The phospholipid (PL) requirement in fish is revealed by enhanced performance when larvae are provided PL-enriched diets. To elucidate the molecular mechanism underlying PL requirement in Atlantic salmon, Salmo salar, were fed a minimal PL diet and tissue samples from major lipid metabolic sites wer...
Published in: | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids |
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Elsevier
2015
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Online Access: | http://hdl.handle.net/1893/22283 https://doi.org/10.1016/j.bbalip.2015.08.006 http://dspace.stir.ac.uk/bitstream/1893/22283/1/Carmona%20et%20al%20Salmon%20PL%20Requirement%20paper%202%20%281%29%20%281%29.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/22283 |
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openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon dietary requirement gene expression phosphoglycerides phospholipids qPCR Aquaculture: Genomics Nutrition |
spellingShingle |
Atlantic salmon dietary requirement gene expression phosphoglycerides phospholipids qPCR Aquaculture: Genomics Nutrition Carmona-Antoñanzas, Greta Taylor, John Martinez-Rubio, Laura Tocher, Douglas R Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry |
topic_facet |
Atlantic salmon dietary requirement gene expression phosphoglycerides phospholipids qPCR Aquaculture: Genomics Nutrition |
description |
The phospholipid (PL) requirement in fish is revealed by enhanced performance when larvae are provided PL-enriched diets. To elucidate the molecular mechanism underlying PL requirement in Atlantic salmon, Salmo salar, were fed a minimal PL diet and tissue samples from major lipid metabolic sites were dissected from fry and parr. In silico analysis and cloning techniques demonstrated that salmon possess a full set of enzymes for the endogenous production of PL. The gene expression data indicated that major PL biosynthetic genes of phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn) and phosphatidylinositol (PtdIns) display lower expression in intestine during the early developmental stage (fry). This is consistent with the hypothesis that the intestine of salmon is immature at the early developmental stage with limited capacity for endogenous PL biosynthesis. The results also indicate that intact PtdCho, PtdEtn and PtdIns are required in the diet at this stage. PtdCho and sphimgomyelin constitute the predominant PL in chylomicrons, involved in the transport of dietary lipids from the intestine to the rest of the body. As sphingomyelin can be produced from PtdCho in intestine of fry, our findings suggest that supplementation of dietary PtdCho alone during early developmental stages of Atlantic salmon would be sufficient to promote chylomicron formation. This would support efficient transport of dietary lipids, including PL precursors, from the intestine to the liver where biosynthesis of PtdEtn, PtdSer, and PtdIns is not compromised as in intestine facilitating efficient utilisation of dietary energy and the endogenous production of membrane PL for the rapidly growing and developing animal. |
author2 |
European Commission Institute of Aquaculture orcid:0000-0003-4370-7922 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Carmona-Antoñanzas, Greta Taylor, John Martinez-Rubio, Laura Tocher, Douglas R |
author_facet |
Carmona-Antoñanzas, Greta Taylor, John Martinez-Rubio, Laura Tocher, Douglas R |
author_sort |
Carmona-Antoñanzas, Greta |
title |
Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry |
title_short |
Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry |
title_full |
Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry |
title_fullStr |
Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry |
title_full_unstemmed |
Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry |
title_sort |
molecular mechanism of dietary phospholipid requirement of atlantic salmon, salmo salar, fry |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/1893/22283 https://doi.org/10.1016/j.bbalip.2015.08.006 http://dspace.stir.ac.uk/bitstream/1893/22283/1/Carmona%20et%20al%20Salmon%20PL%20Requirement%20paper%202%20%281%29%20%281%29.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Carmona-Antoñanzas G, Taylor J, Martinez-Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441. https://doi.org/10.1016/j.bbalip.2015.08.006 ARRAINA KBBE-2001-5-288925 ARRAINA http://hdl.handle.net/1893/22283 doi:10.1016/j.bbalip.2015.08.006 WOS:000362618300002 2-s2.0-84940676570 590186 http://dspace.stir.ac.uk/bitstream/1893/22283/1/Carmona%20et%20al%20Salmon%20PL%20Requirement%20paper%202%20%281%29%20%281%29.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Carmona-Antoñanzas G, Taylor J, Martinez Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry, Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441. DOI:10.1016/j.bbalip.2015.08.006 © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ [Carmona et al Salmon PL Requirement paper 2 (1) (1).pdf] Publisher requires embargo of 12 months after formal publication. |
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CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.bbalip.2015.08.006 |
container_title |
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids |
container_volume |
1851 |
container_issue |
11 |
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1428 |
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1441 |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/22283 2023-05-15T15:30:24+02:00 Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry Carmona-Antoñanzas, Greta Taylor, John Martinez-Rubio, Laura Tocher, Douglas R European Commission Institute of Aquaculture orcid:0000-0003-4370-7922 orcid:0000-0002-8603-9410 2015-11 application/pdf http://hdl.handle.net/1893/22283 https://doi.org/10.1016/j.bbalip.2015.08.006 http://dspace.stir.ac.uk/bitstream/1893/22283/1/Carmona%20et%20al%20Salmon%20PL%20Requirement%20paper%202%20%281%29%20%281%29.pdf en eng Elsevier Carmona-Antoñanzas G, Taylor J, Martinez-Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry. Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441. https://doi.org/10.1016/j.bbalip.2015.08.006 ARRAINA KBBE-2001-5-288925 ARRAINA http://hdl.handle.net/1893/22283 doi:10.1016/j.bbalip.2015.08.006 WOS:000362618300002 2-s2.0-84940676570 590186 http://dspace.stir.ac.uk/bitstream/1893/22283/1/Carmona%20et%20al%20Salmon%20PL%20Requirement%20paper%202%20%281%29%20%281%29.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Carmona-Antoñanzas G, Taylor J, Martinez Rubio L & Tocher DR (2015) Molecular mechanism of dietary phospholipid requirement of Atlantic salmon, Salmo salar, fry, Biochimica et Biophysica Acta (BBA)- Molecular and Cell Biology of Lipids, 1851 (11), pp. 1428-1441. DOI:10.1016/j.bbalip.2015.08.006 © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/ [Carmona et al Salmon PL Requirement paper 2 (1) (1).pdf] Publisher requires embargo of 12 months after formal publication. CC-BY-NC-ND Atlantic salmon dietary requirement gene expression phosphoglycerides phospholipids qPCR Aquaculture: Genomics Nutrition Journal Article AM - Accepted Manuscript 2015 ftunivstirling https://doi.org/10.1016/j.bbalip.2015.08.006 2022-06-13T18:44:43Z The phospholipid (PL) requirement in fish is revealed by enhanced performance when larvae are provided PL-enriched diets. To elucidate the molecular mechanism underlying PL requirement in Atlantic salmon, Salmo salar, were fed a minimal PL diet and tissue samples from major lipid metabolic sites were dissected from fry and parr. In silico analysis and cloning techniques demonstrated that salmon possess a full set of enzymes for the endogenous production of PL. The gene expression data indicated that major PL biosynthetic genes of phosphatidylcholine (PtdCho), phosphatidylethanolamine (PtdEtn) and phosphatidylinositol (PtdIns) display lower expression in intestine during the early developmental stage (fry). This is consistent with the hypothesis that the intestine of salmon is immature at the early developmental stage with limited capacity for endogenous PL biosynthesis. The results also indicate that intact PtdCho, PtdEtn and PtdIns are required in the diet at this stage. PtdCho and sphimgomyelin constitute the predominant PL in chylomicrons, involved in the transport of dietary lipids from the intestine to the rest of the body. As sphingomyelin can be produced from PtdCho in intestine of fry, our findings suggest that supplementation of dietary PtdCho alone during early developmental stages of Atlantic salmon would be sufficient to promote chylomicron formation. This would support efficient transport of dietary lipids, including PL precursors, from the intestine to the liver where biosynthesis of PtdEtn, PtdSer, and PtdIns is not compromised as in intestine facilitating efficient utilisation of dietary energy and the endogenous production of membrane PL for the rapidly growing and developing animal. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1851 11 1428 1441 |