The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids
The main source of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) in our diet is supplied by fish, and an ever-increasing proportion of these are being produced by aquaculture. The drive for the growing market demand and production from sustainable sources has led to the use of high-...
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Other Authors: | , |
Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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University of Stirling
2015
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Subjects: | |
Online Access: | http://hdl.handle.net/1893/21807 http://dspace.stir.ac.uk/bitstream/1893/21807/1/Olga%20Rubio%20PhD%20Thesis.pdf |
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author | Rubio Mejia, Olga Liliana |
author2 | Tocher, Douglas R Conacyt-Mexico (Consejo Nacional de Ciencia y Tecnología) |
author_facet | Rubio Mejia, Olga Liliana |
author_sort | Rubio Mejia, Olga Liliana |
collection | University of Stirling: Stirling Digital Research Repository |
description | The main source of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) in our diet is supplied by fish, and an ever-increasing proportion of these are being produced by aquaculture. The drive for the growing market demand and production from sustainable sources has led to the use of high-energy (fat) diets and, recently, to the replacement of fishmeal and fish oil with non-marine components, such as plant meals and vegetable oils that are devoid of n-3 LC-PUFA. Both changes impact greatly on lipid and fatty acid metabolism in fish, with health implications for the fish and the human consumer. This impact highlights the need to investigate the basic molecular mechanisms underlying the regulation of lipid and fatty acid metabolism in fish, specifically focussing on the pathways of lipid homeostasis and LC-PUFA synthesis. The aim of this study was to develop and utilise Chinook salmon embryo (CHSE-214) cell line as a model for Atlantic salmon, Salmo salar L., to enable an integrated approach to study the biochemical and molecular regulation of lipid metabolism in fish. In particular, α-linolenic acid (LNA, 18:3n-3) and linoleic acid (LOA, 18:2n-6), which are essential fatty acids abundantly found in vegetable oils, and are precursors of LC-PUFA, were supplemented in combination with other fatty acids, to explore the effect of these on total lipid content, lipid class, FA composition and gene expression of CHSE-214 cell line. Total lipid content was extracted, followed by determination of lipid class and fatty acid analyses. Gene expression analyses of transcription/nuclear factors and various target genes in Atlantic salmon, including those involved in pathways of LC-PUFA synthesis and fatty acid oxidation, were carried out. The results demonstrated that CHSE-214 cell line, under experimental conditions, is able to convert LNA to eicosapentaenoic acid (EPA, 20:5n-3), and LOA to arachidonic acid (ARA, 20:4n-6), but not LNA and/or EPA to docosahexaenoic acid (DHA, 22:6n-3), highlighting the activity of ... |
format | Doctoral or Postdoctoral Thesis |
genre | Atlantic salmon Salmo salar |
genre_facet | Atlantic salmon Salmo salar |
id | ftunivstirling:oai:dspace.stir.ac.uk:1893/21807 |
institution | Open Polar |
language | English |
op_collection_id | ftunivstirling |
op_relation | http://hdl.handle.net/1893/21807 http://dspace.stir.ac.uk/bitstream/1893/21807/1/Olga%20Rubio%20PhD%20Thesis.pdf |
publishDate | 2015 |
publisher | University of Stirling |
record_format | openpolar |
spelling | ftunivstirling:oai:dspace.stir.ac.uk:1893/21807 2025-04-06T14:47:51+00:00 The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids Rubio Mejia, Olga Liliana Tocher, Douglas R Conacyt-Mexico (Consejo Nacional de Ciencia y Tecnología) 2015-03-27 application/pdf http://hdl.handle.net/1893/21807 http://dspace.stir.ac.uk/bitstream/1893/21807/1/Olga%20Rubio%20PhD%20Thesis.pdf en eng University of Stirling http://hdl.handle.net/1893/21807 http://dspace.stir.ac.uk/bitstream/1893/21807/1/Olga%20Rubio%20PhD%20Thesis.pdf fatty acid EPA DHA salmon cell line LC-PUFA linolenic acid linoleic acid arachidonic acid CHSE-214 Atlantic salmon Salmonidae Fishes Quality Unsaturated fatty acids in human nutrition Thesis or Dissertation Doctoral Doctor of Philosophy 2015 ftunivstirling 2025-03-11T04:30:58Z The main source of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA) in our diet is supplied by fish, and an ever-increasing proportion of these are being produced by aquaculture. The drive for the growing market demand and production from sustainable sources has led to the use of high-energy (fat) diets and, recently, to the replacement of fishmeal and fish oil with non-marine components, such as plant meals and vegetable oils that are devoid of n-3 LC-PUFA. Both changes impact greatly on lipid and fatty acid metabolism in fish, with health implications for the fish and the human consumer. This impact highlights the need to investigate the basic molecular mechanisms underlying the regulation of lipid and fatty acid metabolism in fish, specifically focussing on the pathways of lipid homeostasis and LC-PUFA synthesis. The aim of this study was to develop and utilise Chinook salmon embryo (CHSE-214) cell line as a model for Atlantic salmon, Salmo salar L., to enable an integrated approach to study the biochemical and molecular regulation of lipid metabolism in fish. In particular, α-linolenic acid (LNA, 18:3n-3) and linoleic acid (LOA, 18:2n-6), which are essential fatty acids abundantly found in vegetable oils, and are precursors of LC-PUFA, were supplemented in combination with other fatty acids, to explore the effect of these on total lipid content, lipid class, FA composition and gene expression of CHSE-214 cell line. Total lipid content was extracted, followed by determination of lipid class and fatty acid analyses. Gene expression analyses of transcription/nuclear factors and various target genes in Atlantic salmon, including those involved in pathways of LC-PUFA synthesis and fatty acid oxidation, were carried out. The results demonstrated that CHSE-214 cell line, under experimental conditions, is able to convert LNA to eicosapentaenoic acid (EPA, 20:5n-3), and LOA to arachidonic acid (ARA, 20:4n-6), but not LNA and/or EPA to docosahexaenoic acid (DHA, 22:6n-3), highlighting the activity of ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository |
spellingShingle | fatty acid EPA DHA salmon cell line LC-PUFA linolenic acid linoleic acid arachidonic acid CHSE-214 Atlantic salmon Salmonidae Fishes Quality Unsaturated fatty acids in human nutrition Rubio Mejia, Olga Liliana The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
title | The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
title_full | The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
title_fullStr | The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
title_full_unstemmed | The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
title_short | The CHSE-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
title_sort | chse-214 salmon cell line as a model to study molecular regulation of long-chain polyunsaturated fatty acid biosynthesis in salmonids |
topic | fatty acid EPA DHA salmon cell line LC-PUFA linolenic acid linoleic acid arachidonic acid CHSE-214 Atlantic salmon Salmonidae Fishes Quality Unsaturated fatty acids in human nutrition |
topic_facet | fatty acid EPA DHA salmon cell line LC-PUFA linolenic acid linoleic acid arachidonic acid CHSE-214 Atlantic salmon Salmonidae Fishes Quality Unsaturated fatty acids in human nutrition |
url | http://hdl.handle.net/1893/21807 http://dspace.stir.ac.uk/bitstream/1893/21807/1/Olga%20Rubio%20PhD%20Thesis.pdf |