Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica
The capacity for endogenous production of LC-PUFA from PUFA in euryhaline or diadromous fish is largely unknown other than for Atlantic salmon (Salmo salar), an anadromous species, which displays a freshwater pattern. The aim of the present study was to characterize the enzymes of the LC-PUFA pathwa...
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Online Access: | http://hdl.handle.net/1893/21470 https://doi.org/10.1016/j.aquaculture.2014.07.016 http://dspace.stir.ac.uk/bitstream/1893/21470/1/Eel%20Final.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/21470 2023-05-15T15:31:40+02:00 Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica Wang, Shuqi Monroig, Oscar Tang, Guoxia Zhang, Liang You, Cuihong Tocher, Douglas R Li, Yuanyou National Natural Science Foundation of China European Community Framework Programme Shantou University Institute of Aquaculture orcid:0000-0001-8712-0440 orcid:0000-0002-8603-9410 2014-10 application/pdf http://hdl.handle.net/1893/21470 https://doi.org/10.1016/j.aquaculture.2014.07.016 http://dspace.stir.ac.uk/bitstream/1893/21470/1/Eel%20Final.pdf en eng Elsevier Wang S, Monroig O, Tang G, Zhang L, You C, Tocher DR & Li Y (2014) Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica. Aquaculture, 434, pp. 57-65. https://doi.org/10.1016/j.aquaculture.2014.07.016 http://hdl.handle.net/1893/21470 doi:10.1016/j.aquaculture.2014.07.016 WOS:000345058700009 2-s2.0-84905682102 603891 http://dspace.stir.ac.uk/bitstream/1893/21470/1/Eel%20Final.pdf This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. this is the author’s version of a work that was accepted for publication in Aquaculture. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquaculture, Volume 434, 20 October 2014, Pages 57–65. doi:10.1016/j.aquaculture.2014.07.016 [Eel Final.pdf] Publisher requires embargo of 24 months after formal publication. Biosynthesis Catadromy Elovl5 Fads2 Japanese eel Long-chain polyunsaturated fatty acids Journal Article AM - Accepted Manuscript 2014 ftunivstirling https://doi.org/10.1016/j.aquaculture.2014.07.016 2022-06-13T18:46:11Z The capacity for endogenous production of LC-PUFA from PUFA in euryhaline or diadromous fish is largely unknown other than for Atlantic salmon (Salmo salar), an anadromous species, which displays a freshwater pattern. The aim of the present study was to characterize the enzymes of the LC-PUFA pathway in Japanese eel (Anguilla japonica), the most important catadromous species currently being farmed. cDNAs of two key genes were cloned and functional assays showed they encoded a desaturase (Fads2) with D6 and D8 activity and an elongase (Elovl5) with activity towards C18 and C20 PUFA, with activities similar to marine fish and an D6/D8 activity ratio similar to Atlantic salmon. Furthermore, tissue distribution of the mRNA showed a clear marine pattern with highest expression in brain and eye. Phylogenetic analysis placed the eel cDNAs in line with classical taxonomy. The data suggest that diadromous species display a pattern of LC-PUFA biosynthesis capacity that likely reflects the environmental and nutritional influence of their early life stages rather than those of adult fish. Future studies aim to establish the full range of PUFA desaturases and elongases in Japanese eel and to provide further insight to the importance and relevance of LC-PUFA biosynthesis in fish species and the influence of diadromy. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Aquaculture 434 57 65 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Biosynthesis Catadromy Elovl5 Fads2 Japanese eel Long-chain polyunsaturated fatty acids |
spellingShingle |
Biosynthesis Catadromy Elovl5 Fads2 Japanese eel Long-chain polyunsaturated fatty acids Wang, Shuqi Monroig, Oscar Tang, Guoxia Zhang, Liang You, Cuihong Tocher, Douglas R Li, Yuanyou Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica |
topic_facet |
Biosynthesis Catadromy Elovl5 Fads2 Japanese eel Long-chain polyunsaturated fatty acids |
description |
The capacity for endogenous production of LC-PUFA from PUFA in euryhaline or diadromous fish is largely unknown other than for Atlantic salmon (Salmo salar), an anadromous species, which displays a freshwater pattern. The aim of the present study was to characterize the enzymes of the LC-PUFA pathway in Japanese eel (Anguilla japonica), the most important catadromous species currently being farmed. cDNAs of two key genes were cloned and functional assays showed they encoded a desaturase (Fads2) with D6 and D8 activity and an elongase (Elovl5) with activity towards C18 and C20 PUFA, with activities similar to marine fish and an D6/D8 activity ratio similar to Atlantic salmon. Furthermore, tissue distribution of the mRNA showed a clear marine pattern with highest expression in brain and eye. Phylogenetic analysis placed the eel cDNAs in line with classical taxonomy. The data suggest that diadromous species display a pattern of LC-PUFA biosynthesis capacity that likely reflects the environmental and nutritional influence of their early life stages rather than those of adult fish. Future studies aim to establish the full range of PUFA desaturases and elongases in Japanese eel and to provide further insight to the importance and relevance of LC-PUFA biosynthesis in fish species and the influence of diadromy. |
author2 |
National Natural Science Foundation of China European Community Framework Programme Shantou University Institute of Aquaculture orcid:0000-0001-8712-0440 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Wang, Shuqi Monroig, Oscar Tang, Guoxia Zhang, Liang You, Cuihong Tocher, Douglas R Li, Yuanyou |
author_facet |
Wang, Shuqi Monroig, Oscar Tang, Guoxia Zhang, Liang You, Cuihong Tocher, Douglas R Li, Yuanyou |
author_sort |
Wang, Shuqi |
title |
Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica |
title_short |
Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica |
title_full |
Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica |
title_fullStr |
Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica |
title_full_unstemmed |
Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica |
title_sort |
investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: functional characterization of fatty acyl desaturase (fads2) and elovl5 elongase in the catadromous species, japanese eel anguilla japonica |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/1893/21470 https://doi.org/10.1016/j.aquaculture.2014.07.016 http://dspace.stir.ac.uk/bitstream/1893/21470/1/Eel%20Final.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Wang S, Monroig O, Tang G, Zhang L, You C, Tocher DR & Li Y (2014) Investigating long-chain polyunsaturated fatty acid biosynthesis in teleost fish: Functional characterization of fatty acyl desaturase (Fads2) and Elovl5 elongase in the catadromous species, Japanese eel Anguilla japonica. Aquaculture, 434, pp. 57-65. https://doi.org/10.1016/j.aquaculture.2014.07.016 http://hdl.handle.net/1893/21470 doi:10.1016/j.aquaculture.2014.07.016 WOS:000345058700009 2-s2.0-84905682102 603891 http://dspace.stir.ac.uk/bitstream/1893/21470/1/Eel%20Final.pdf |
op_rights |
This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. this is the author’s version of a work that was accepted for publication in Aquaculture. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Aquaculture, Volume 434, 20 October 2014, Pages 57–65. doi:10.1016/j.aquaculture.2014.07.016 [Eel Final.pdf] Publisher requires embargo of 24 months after formal publication. |
op_doi |
https://doi.org/10.1016/j.aquaculture.2014.07.016 |
container_title |
Aquaculture |
container_volume |
434 |
container_start_page |
57 |
op_container_end_page |
65 |
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1766362193195958272 |