Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.)
In mammals, several genes involved in liver lipid and cholesterol homeostasis are rhythmically expressed with expression shown to be regulated by clock genes via Rev-erb 1α. In order to elucidate clock gene regulation of genes involved in lipid metabolism in Atlantic salmon (Salmo salar L.), the orp...
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ftunivstirling:oai:dspace.stir.ac.uk:1893/21211 2024-06-02T08:03:29+00:00 Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) Betancor, Monica McStay, Elsbeth Minghetti, Matteo Migaud, Herve Tocher, Douglas R Davie, Andrew Institute of Aquaculture orcid:0000-0003-1626-7458 orcid:0000-0002-5404-7512 orcid:0000-0002-8603-9410 orcid:0000-0002-9524-618X 2014-09-03 application/pdf http://hdl.handle.net/1893/21211 https://doi.org/10.1371/journal.pone.0106739 http://dspace.stir.ac.uk/bitstream/1893/21211/1/Plos%20One%202014.pdf en eng Public Library of Science Betancor M, McStay E, Minghetti M, Migaud H, Tocher DR & Davie A (2014) Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). PLoS ONE, 9 (9), Art. No.: e106739. https://doi.org/10.1371/journal.pone.0106739 e106739 http://hdl.handle.net/1893/21211 doi:10.1371/journal.pone.0106739 WOS:000341257700113 2-s2.0-84906978490 614267 http://dspace.stir.ac.uk/bitstream/1893/21211/1/Plos%20One%202014.pdf © 2014 Betancor et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ Journal Article VoR - Version of Record 2014 ftunivstirling https://doi.org/10.1371/journal.pone.0106739 2024-05-07T04:30:11Z In mammals, several genes involved in liver lipid and cholesterol homeostasis are rhythmically expressed with expression shown to be regulated by clock genes via Rev-erb 1α. In order to elucidate clock gene regulation of genes involved in lipid metabolism in Atlantic salmon (Salmo salar L.), the orphan nuclear receptor Rev-erb 1α was cloned and 24 h expression of clock genes, transcription factors and genes involved in cholesterol and lipid metabolism determined in liver of parr acclimated to a long-day photoperiod, which was previously shown to elicit rhythmic clock gene expression in the brain. Of the 31 genes analysed, significant daily expression was demonstrated in the clock gene Bmal1, transcription factor genes Srebp1, Lxr, Pparα and Pparγ, and several lipid metabolism genes Hmgcr, Ipi, ApoCII and El. The possible regulatory mechanisms and pathways, and the functional significance of these patterns of expression were discussed. Importantly and in contrast to mammals, Per1, Per2, Fas, Srebp2, Cyp71α and Rev-erb 1α did not display significant daily rhythmicity in salmon. The present study is the first report characterising 24 h profiles of gene expression in liver of Atlantic salmon. However, more importantly, the predominant role of lipids in the nutrition and metabolism of fish, and of feed efficiency in determining farming economics, means that daily rhythmicity in the regulation of lipid metabolism will be an area of considerable interest for future research in commercially important species. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository PLoS ONE 9 9 e106739 |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
description |
In mammals, several genes involved in liver lipid and cholesterol homeostasis are rhythmically expressed with expression shown to be regulated by clock genes via Rev-erb 1α. In order to elucidate clock gene regulation of genes involved in lipid metabolism in Atlantic salmon (Salmo salar L.), the orphan nuclear receptor Rev-erb 1α was cloned and 24 h expression of clock genes, transcription factors and genes involved in cholesterol and lipid metabolism determined in liver of parr acclimated to a long-day photoperiod, which was previously shown to elicit rhythmic clock gene expression in the brain. Of the 31 genes analysed, significant daily expression was demonstrated in the clock gene Bmal1, transcription factor genes Srebp1, Lxr, Pparα and Pparγ, and several lipid metabolism genes Hmgcr, Ipi, ApoCII and El. The possible regulatory mechanisms and pathways, and the functional significance of these patterns of expression were discussed. Importantly and in contrast to mammals, Per1, Per2, Fas, Srebp2, Cyp71α and Rev-erb 1α did not display significant daily rhythmicity in salmon. The present study is the first report characterising 24 h profiles of gene expression in liver of Atlantic salmon. However, more importantly, the predominant role of lipids in the nutrition and metabolism of fish, and of feed efficiency in determining farming economics, means that daily rhythmicity in the regulation of lipid metabolism will be an area of considerable interest for future research in commercially important species. |
author2 |
Institute of Aquaculture orcid:0000-0003-1626-7458 orcid:0000-0002-5404-7512 orcid:0000-0002-8603-9410 orcid:0000-0002-9524-618X |
format |
Article in Journal/Newspaper |
author |
Betancor, Monica McStay, Elsbeth Minghetti, Matteo Migaud, Herve Tocher, Douglas R Davie, Andrew |
spellingShingle |
Betancor, Monica McStay, Elsbeth Minghetti, Matteo Migaud, Herve Tocher, Douglas R Davie, Andrew Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
author_facet |
Betancor, Monica McStay, Elsbeth Minghetti, Matteo Migaud, Herve Tocher, Douglas R Davie, Andrew |
author_sort |
Betancor, Monica |
title |
Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_short |
Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_full |
Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_fullStr |
Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.) |
title_sort |
daily rhythms in expression of genes of hepatic lipid metabolism in atlantic salmon (salmo salar l.) |
publisher |
Public Library of Science |
publishDate |
2014 |
url |
http://hdl.handle.net/1893/21211 https://doi.org/10.1371/journal.pone.0106739 http://dspace.stir.ac.uk/bitstream/1893/21211/1/Plos%20One%202014.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Betancor M, McStay E, Minghetti M, Migaud H, Tocher DR & Davie A (2014) Daily rhythms in expression of genes of hepatic lipid metabolism in Atlantic salmon (Salmo salar L.). PLoS ONE, 9 (9), Art. No.: e106739. https://doi.org/10.1371/journal.pone.0106739 e106739 http://hdl.handle.net/1893/21211 doi:10.1371/journal.pone.0106739 WOS:000341257700113 2-s2.0-84906978490 614267 http://dspace.stir.ac.uk/bitstream/1893/21211/1/Plos%20One%202014.pdf |
op_rights |
© 2014 Betancor et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0106739 |
container_title |
PLoS ONE |
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9 |
container_issue |
9 |
container_start_page |
e106739 |
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1800748022814998528 |