Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways
The effects of chronic long term exposure to multiply-polluted environments on fish are not well understood, but environmental surveys suggest that such exposure may cause a variety of pathologies, including cancers. Transcriptomic profiling has recently been used to assess gene expression in Europe...
Published in: | Aquatic Toxicology |
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Main Authors: | , , , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2010
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Online Access: | http://hdl.handle.net/1893/2051 https://doi.org/10.1016/j.aquatox.2009.10.025 http://www.sciencedirect.com/science/journal/0166445X http://dspace.stir.ac.uk/bitstream/1893/2051/1/LeaverAqTox2009TextRevisedforSTORRE.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/2051 |
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record_format |
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Open Polar |
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University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
ecotoxicolgy microarray flounder sediment Diablo cyp1A Lipoproteins Fish Fishes Feeding and feeds Dietary supplements Atlantic salmon |
spellingShingle |
ecotoxicolgy microarray flounder sediment Diablo cyp1A Lipoproteins Fish Fishes Feeding and feeds Dietary supplements Atlantic salmon Leaver, Michael Diab, Amer Boukouvala, Evridiki Williams, Tim D Chipman, James Kevin Moffat, Colin F Robinson, Craig D George, Stephen Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
topic_facet |
ecotoxicolgy microarray flounder sediment Diablo cyp1A Lipoproteins Fish Fishes Feeding and feeds Dietary supplements Atlantic salmon |
description |
The effects of chronic long term exposure to multiply-polluted environments on fish are not well understood, but environmental surveys suggest that such exposure may cause a variety of pathologies, including cancers. Transcriptomic profiling has recently been used to assess gene expression in European flounder (Platichthys flesus) living in several polluted and clean estuaries. However, the gene expression changes detected were not unequivocally elicited by pollution, most likely due to the confounding effects of natural estuarine ecosystem variables. In this study flounder from an uncontaminated estuary were held on clean or polluted sediments in mesocosms, allowing control of variables such as salinity, temperature, and diet. After 7 months flounder were removed from each mesocosm and hepatocytes prepared from fish exposed to clean or polluted sediments. The hepatocytes were treated with benzo(a)pyrene (BAP), estradiol (E2), copper, a mixture of these three, or with the vehicle DMSO. A flounder cDNA microarray was then used to measure hepatocyte transcript abundance after each treatment. The results show that long term chronic exposure to a multiply-polluted sediment causes increases in the expression of mRNAs coding for proteins of the endogenous apoptotic program, of innate immunity and inflammation. Contrary to expectation, the expression of mRNAs which are commonly used as biomarkers of environmental exposure to particular contaminants were not changed, or were changed contrary to expectation. However, acute treatment of hepatocytes from flounder from both clean and polluted sediments with BAP or E2 caused the expected changes in the expression of these biomarkers. Thus transcriptomic analysis of flounder exposed long-term to chronic pollution causes a different pattern of gene expression than in fish acutely treated with single chemicals, and reveals novel potential biomarkers of environmental contaminant exposure. These novel biomarkers include Diablo, a gene involved in apoptotic pathways and highly ... |
author2 |
Institute of Aquaculture National Agricultural Research Foundation University of Birmingham Scottish Government - Enterprise, Environment & Digital - Marine Scotland orcid:0000-0002-3155-0844 |
format |
Article in Journal/Newspaper |
author |
Leaver, Michael Diab, Amer Boukouvala, Evridiki Williams, Tim D Chipman, James Kevin Moffat, Colin F Robinson, Craig D George, Stephen |
author_facet |
Leaver, Michael Diab, Amer Boukouvala, Evridiki Williams, Tim D Chipman, James Kevin Moffat, Colin F Robinson, Craig D George, Stephen |
author_sort |
Leaver, Michael |
title |
Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
title_short |
Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
title_full |
Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
title_fullStr |
Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
title_full_unstemmed |
Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
title_sort |
hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways |
publisher |
Elsevier |
publishDate |
2010 |
url |
http://hdl.handle.net/1893/2051 https://doi.org/10.1016/j.aquatox.2009.10.025 http://www.sciencedirect.com/science/journal/0166445X http://dspace.stir.ac.uk/bitstream/1893/2051/1/LeaverAqTox2009TextRevisedforSTORRE.pdf |
long_lat |
ENVELOPE(-57.289,-57.289,-63.799,-63.799) |
geographic |
Diablo |
geographic_facet |
Diablo |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Leaver M, Diab A, Boukouvala E, Williams TD, Chipman JK, Moffat CF, Robinson CD & George S (2010) Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways. Aquatic Toxicology, 96 (3), pp. 234-245. http://www.sciencedirect.com/science/journal/0166445X; https://doi.org/10.1016/j.aquatox.2009.10.025 http://hdl.handle.net/1893/2051 doi:10.1016/j.aquatox.2009.10.025 http://www.sciencedirect.com/science/journal/0166445X WOS:000274950000007 2-s2.0-75449092200 837688 http://dspace.stir.ac.uk/bitstream/1893/2051/1/LeaverAqTox2009TextRevisedforSTORRE.pdf |
op_rights |
Published in Aquatic Toxicology by Elsevier. |
op_doi |
https://doi.org/10.1016/j.aquatox.2009.10.025 |
container_title |
Aquatic Toxicology |
container_volume |
96 |
container_issue |
3 |
container_start_page |
234 |
op_container_end_page |
245 |
_version_ |
1766363395255173120 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/2051 2023-05-15T15:32:55+02:00 Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways Leaver, Michael Diab, Amer Boukouvala, Evridiki Williams, Tim D Chipman, James Kevin Moffat, Colin F Robinson, Craig D George, Stephen Institute of Aquaculture National Agricultural Research Foundation University of Birmingham Scottish Government - Enterprise, Environment & Digital - Marine Scotland orcid:0000-0002-3155-0844 2010-02-08 application/pdf http://hdl.handle.net/1893/2051 https://doi.org/10.1016/j.aquatox.2009.10.025 http://www.sciencedirect.com/science/journal/0166445X http://dspace.stir.ac.uk/bitstream/1893/2051/1/LeaverAqTox2009TextRevisedforSTORRE.pdf en eng Elsevier Leaver M, Diab A, Boukouvala E, Williams TD, Chipman JK, Moffat CF, Robinson CD & George S (2010) Hepatic gene expression in flounder chronically exposed to multiply polluted estuarine sediment: Absence of classical exposure ‘biomarker’ signals and induction of inflammatory, innate immune and apoptotic pathways. Aquatic Toxicology, 96 (3), pp. 234-245. http://www.sciencedirect.com/science/journal/0166445X; https://doi.org/10.1016/j.aquatox.2009.10.025 http://hdl.handle.net/1893/2051 doi:10.1016/j.aquatox.2009.10.025 http://www.sciencedirect.com/science/journal/0166445X WOS:000274950000007 2-s2.0-75449092200 837688 http://dspace.stir.ac.uk/bitstream/1893/2051/1/LeaverAqTox2009TextRevisedforSTORRE.pdf Published in Aquatic Toxicology by Elsevier. ecotoxicolgy microarray flounder sediment Diablo cyp1A Lipoproteins Fish Fishes Feeding and feeds Dietary supplements Atlantic salmon Journal Article AM - Accepted Manuscript 2010 ftunivstirling https://doi.org/10.1016/j.aquatox.2009.10.025 2022-06-13T18:43:37Z The effects of chronic long term exposure to multiply-polluted environments on fish are not well understood, but environmental surveys suggest that such exposure may cause a variety of pathologies, including cancers. Transcriptomic profiling has recently been used to assess gene expression in European flounder (Platichthys flesus) living in several polluted and clean estuaries. However, the gene expression changes detected were not unequivocally elicited by pollution, most likely due to the confounding effects of natural estuarine ecosystem variables. In this study flounder from an uncontaminated estuary were held on clean or polluted sediments in mesocosms, allowing control of variables such as salinity, temperature, and diet. After 7 months flounder were removed from each mesocosm and hepatocytes prepared from fish exposed to clean or polluted sediments. The hepatocytes were treated with benzo(a)pyrene (BAP), estradiol (E2), copper, a mixture of these three, or with the vehicle DMSO. A flounder cDNA microarray was then used to measure hepatocyte transcript abundance after each treatment. The results show that long term chronic exposure to a multiply-polluted sediment causes increases in the expression of mRNAs coding for proteins of the endogenous apoptotic program, of innate immunity and inflammation. Contrary to expectation, the expression of mRNAs which are commonly used as biomarkers of environmental exposure to particular contaminants were not changed, or were changed contrary to expectation. However, acute treatment of hepatocytes from flounder from both clean and polluted sediments with BAP or E2 caused the expected changes in the expression of these biomarkers. Thus transcriptomic analysis of flounder exposed long-term to chronic pollution causes a different pattern of gene expression than in fish acutely treated with single chemicals, and reveals novel potential biomarkers of environmental contaminant exposure. These novel biomarkers include Diablo, a gene involved in apoptotic pathways and highly ... Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository Diablo ENVELOPE(-57.289,-57.289,-63.799,-63.799) Aquatic Toxicology 96 3 234 245 |