An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon
BACKGROUND The ability to produce physiologically critical LC-PUFA from dietary fatty acids differs greatly among teleost species, and is dependent on the possession and expression of fatty acyl desaturase and elongase genes. Atlantic salmon, as a result of a recently duplicated genome, have more of...
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BioMed Central Ltd
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Online Access: | http://hdl.handle.net/1893/17869 https://doi.org/10.1186/1471-2148-13-85 http://dspace.stir.ac.uk/bitstream/1893/17869/1/An%20evolutionary%20perspective%20on%20Elovl5%20fatty%20acid%20elongase.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/17869 2023-05-15T15:29:58+02:00 An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon Carmona-Antonanzas, Greta Tocher, Douglas R Taggart, John Leaver, Michael Institute of Aquaculture orcid:0000-0002-8603-9410 orcid:0000-0002-3843-9663 orcid:0000-0002-3155-0844 2013-04 application/pdf http://hdl.handle.net/1893/17869 https://doi.org/10.1186/1471-2148-13-85 http://dspace.stir.ac.uk/bitstream/1893/17869/1/An%20evolutionary%20perspective%20on%20Elovl5%20fatty%20acid%20elongase.pdf en eng BioMed Central Ltd Carmona-Antonanzas G, Tocher DR, Taggart J & Leaver M (2013) An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon. BMC Evolutionary Biology, 13, Art. No.: 85. https://doi.org/10.1186/1471-2148-13-85 85 http://hdl.handle.net/1893/17869 doi:10.1186/1471-2148-13-85 23597093 WOS:000318368500001 2-s2.0-84876290563 676259 http://dspace.stir.ac.uk/bitstream/1893/17869/1/An%20evolutionary%20perspective%20on%20Elovl5%20fatty%20acid%20elongase.pdf © 2013 Carmona-Antoñanzas et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/ CC-BY Atlantic salmon Elongase of very long-chain fatty acids Northern pike Paralogous genes Whole-genome duplication Journal Article VoR - Version of Record 2013 ftunivstirling https://doi.org/10.1186/1471-2148-13-85 2022-06-13T18:45:37Z BACKGROUND The ability to produce physiologically critical LC-PUFA from dietary fatty acids differs greatly among teleost species, and is dependent on the possession and expression of fatty acyl desaturase and elongase genes. Atlantic salmon, as a result of a recently duplicated genome, have more of these enzymes than other fish. Recent phylogenetic studies show that Northern pike represents the closest extant relative of the preduplicated ancestral salmonid. Here we characterise a pike fatty acyl elongase, elovl5, and compare it to Atlantic salmon elovl5a and elovl5b duplicates. RESULTS Phylogenetic analyses show that Atlantic salmon paralogs are evolving symmetrically, and they have been retained in the genome by purifying selection. Heterologous expression in yeast showed that Northern pike Elovl5 activity is indistinguishable from that of the salmon paralogs, efficiently elongating C18 and C20 substrates. However, in contrast to salmon, pike elovl5 was predominantly expressed in brain with negligible expression in liver and intestine. CONCLUSIONS We suggest that the predominant expression of Elovl5b in salmon liver and Elovl5a in salmon intestine is an adaptation, enabled by genome duplication, to a diet rich in terrestrial invertebrates which are relatively poor in LC-PUFA. Pike have retained an ancestral expression profile which supports the maintenance of PUFA in the brain but, due to a highly piscivorous LC-PUFA-rich diet, is not required in liver and intestine. Thus, the characterisation of elovl5 in Northern pike provides insights into the evolutionary divergence of duplicated genes, and the ecological adaptations of salmonids which have enabled colonisation of nutrient poor freshwaters. Article in Journal/Newspaper Atlantic salmon University of Stirling: Stirling Digital Research Repository BMC Evolutionary Biology 13 1 85 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon Elongase of very long-chain fatty acids Northern pike Paralogous genes Whole-genome duplication |
spellingShingle |
Atlantic salmon Elongase of very long-chain fatty acids Northern pike Paralogous genes Whole-genome duplication Carmona-Antonanzas, Greta Tocher, Douglas R Taggart, John Leaver, Michael An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon |
topic_facet |
Atlantic salmon Elongase of very long-chain fatty acids Northern pike Paralogous genes Whole-genome duplication |
description |
BACKGROUND The ability to produce physiologically critical LC-PUFA from dietary fatty acids differs greatly among teleost species, and is dependent on the possession and expression of fatty acyl desaturase and elongase genes. Atlantic salmon, as a result of a recently duplicated genome, have more of these enzymes than other fish. Recent phylogenetic studies show that Northern pike represents the closest extant relative of the preduplicated ancestral salmonid. Here we characterise a pike fatty acyl elongase, elovl5, and compare it to Atlantic salmon elovl5a and elovl5b duplicates. RESULTS Phylogenetic analyses show that Atlantic salmon paralogs are evolving symmetrically, and they have been retained in the genome by purifying selection. Heterologous expression in yeast showed that Northern pike Elovl5 activity is indistinguishable from that of the salmon paralogs, efficiently elongating C18 and C20 substrates. However, in contrast to salmon, pike elovl5 was predominantly expressed in brain with negligible expression in liver and intestine. CONCLUSIONS We suggest that the predominant expression of Elovl5b in salmon liver and Elovl5a in salmon intestine is an adaptation, enabled by genome duplication, to a diet rich in terrestrial invertebrates which are relatively poor in LC-PUFA. Pike have retained an ancestral expression profile which supports the maintenance of PUFA in the brain but, due to a highly piscivorous LC-PUFA-rich diet, is not required in liver and intestine. Thus, the characterisation of elovl5 in Northern pike provides insights into the evolutionary divergence of duplicated genes, and the ecological adaptations of salmonids which have enabled colonisation of nutrient poor freshwaters. |
author2 |
Institute of Aquaculture orcid:0000-0002-8603-9410 orcid:0000-0002-3843-9663 orcid:0000-0002-3155-0844 |
format |
Article in Journal/Newspaper |
author |
Carmona-Antonanzas, Greta Tocher, Douglas R Taggart, John Leaver, Michael |
author_facet |
Carmona-Antonanzas, Greta Tocher, Douglas R Taggart, John Leaver, Michael |
author_sort |
Carmona-Antonanzas, Greta |
title |
An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon |
title_short |
An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon |
title_full |
An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon |
title_fullStr |
An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon |
title_full_unstemmed |
An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon |
title_sort |
evolutionary perspective on elovl5 fatty acid elongase: comparison of northern pike and duplicated paralogs from atlantic salmon |
publisher |
BioMed Central Ltd |
publishDate |
2013 |
url |
http://hdl.handle.net/1893/17869 https://doi.org/10.1186/1471-2148-13-85 http://dspace.stir.ac.uk/bitstream/1893/17869/1/An%20evolutionary%20perspective%20on%20Elovl5%20fatty%20acid%20elongase.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
Carmona-Antonanzas G, Tocher DR, Taggart J & Leaver M (2013) An evolutionary perspective on Elovl5 fatty acid elongase: comparison of Northern pike and duplicated paralogs from Atlantic salmon. BMC Evolutionary Biology, 13, Art. No.: 85. https://doi.org/10.1186/1471-2148-13-85 85 http://hdl.handle.net/1893/17869 doi:10.1186/1471-2148-13-85 23597093 WOS:000318368500001 2-s2.0-84876290563 676259 http://dspace.stir.ac.uk/bitstream/1893/17869/1/An%20evolutionary%20perspective%20on%20Elovl5%20fatty%20acid%20elongase.pdf |
op_rights |
© 2013 Carmona-Antoñanzas et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/1471-2148-13-85 |
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BMC Evolutionary Biology |
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13 |
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1 |
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85 |
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1766360411862466560 |