Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters
Fish vary in ability to biosynthesise n-3 long-chain polyunsaturated fatty acids (LC-PUFA), with marine fish such as cod being inefficient in comparison to freshwater and salmonid fish. We investigated differences in the gene promoters of Δ6 fatty acyl desaturase (Δ6 FAD), a critical enzyme in LC-PU...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/1707 2023-05-15T15:27:43+02:00 Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters Zheng, Xiaozhong Leaver, Michael Tocher, Douglas R Institute of Aquaculture orcid:0000-0002-3155-0844 orcid:0000-0002-8603-9410 2009-11 application/pdf http://hdl.handle.net/1893/1707 https://doi.org/10.1016/j.cbpb.2009.06.010 http://www.sciencedirect.com/science/journal/10964959 http://dspace.stir.ac.uk/bitstream/1893/1707/1/Zheng%20et%20al%202009-2.pdf en eng Elsevier Zheng X, Leaver M & Tocher DR (2009) Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 154 (3), pp. 255-263. http://www.sciencedirect.com/science/journal/10964959; https://doi.org/10.1016/j.cbpb.2009.06.010 http://hdl.handle.net/1893/1707 doi:10.1016/j.cbpb.2009.06.010 http://www.sciencedirect.com/science/journal/10964959 WOS:000270767700001 2-s2.0-70149086891 835482 http://dspace.stir.ac.uk/bitstream/1893/1707/1/Zheng%20et%20al%202009-2.pdf Published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology by Elsevier. long-chain polyunsaturated fatty acids biosynthesis fatty acyl delta 6 desaturase promoters regulation transcription factors Fatty acids Metabolism Regulation Lipoproteins Fish Linoleic acid Physiological effect Unsaturated fatty acids in human nutrition Fishes Feeding and feeds Dietary supplements Fishes Quality Fishes Health Journal Article AM - Accepted Manuscript 2009 ftunivstirling https://doi.org/10.1016/j.cbpb.2009.06.010 2022-06-13T18:45:33Z Fish vary in ability to biosynthesise n-3 long-chain polyunsaturated fatty acids (LC-PUFA), with marine fish such as cod being inefficient in comparison to freshwater and salmonid fish. We investigated differences in the gene promoters of Δ6 fatty acyl desaturase (Δ6 FAD), a critical enzyme in LC-PUFA biosynthesis, in cod and salmon. Progressive deletions and targeted mutations of the promoters were tested for activity in a transfected fish cell line under low or high LC-PUFA treatment, and regions sufficient to direct transcription were identified. Comparison of these regions with sequences of corresponding regions of Δ6 FAD genes from mammals, amphibians and fish indicated a remarkable conservation of binding sites for SREBPs and NF-Y. In addition to these sites, a site was identified in salmon with similarity to that recognised by Sp1 transcription factor, and which was required for full expression of the salmon Δ6 FAD gene. The cod promoter was less active and lacked the Sp1 site. Eicosapentaenoic acid suppressed LC-PUFA synthesis in AS cells and also suppressed activity of the salmon Δ6 FAD promoter although this activity was likely mediated through sites other than Sp1, possibly similar to those recognised by NF-Y and SREBP transcription factors. Article in Journal/Newspaper atlantic cod Atlantic salmon Gadus morhua Salmo salar University of Stirling: Stirling Digital Research Repository Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 154 3 255 263 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
long-chain polyunsaturated fatty acids biosynthesis fatty acyl delta 6 desaturase promoters regulation transcription factors Fatty acids Metabolism Regulation Lipoproteins Fish Linoleic acid Physiological effect Unsaturated fatty acids in human nutrition Fishes Feeding and feeds Dietary supplements Fishes Quality Fishes Health |
spellingShingle |
long-chain polyunsaturated fatty acids biosynthesis fatty acyl delta 6 desaturase promoters regulation transcription factors Fatty acids Metabolism Regulation Lipoproteins Fish Linoleic acid Physiological effect Unsaturated fatty acids in human nutrition Fishes Feeding and feeds Dietary supplements Fishes Quality Fishes Health Zheng, Xiaozhong Leaver, Michael Tocher, Douglas R Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters |
topic_facet |
long-chain polyunsaturated fatty acids biosynthesis fatty acyl delta 6 desaturase promoters regulation transcription factors Fatty acids Metabolism Regulation Lipoproteins Fish Linoleic acid Physiological effect Unsaturated fatty acids in human nutrition Fishes Feeding and feeds Dietary supplements Fishes Quality Fishes Health |
description |
Fish vary in ability to biosynthesise n-3 long-chain polyunsaturated fatty acids (LC-PUFA), with marine fish such as cod being inefficient in comparison to freshwater and salmonid fish. We investigated differences in the gene promoters of Δ6 fatty acyl desaturase (Δ6 FAD), a critical enzyme in LC-PUFA biosynthesis, in cod and salmon. Progressive deletions and targeted mutations of the promoters were tested for activity in a transfected fish cell line under low or high LC-PUFA treatment, and regions sufficient to direct transcription were identified. Comparison of these regions with sequences of corresponding regions of Δ6 FAD genes from mammals, amphibians and fish indicated a remarkable conservation of binding sites for SREBPs and NF-Y. In addition to these sites, a site was identified in salmon with similarity to that recognised by Sp1 transcription factor, and which was required for full expression of the salmon Δ6 FAD gene. The cod promoter was less active and lacked the Sp1 site. Eicosapentaenoic acid suppressed LC-PUFA synthesis in AS cells and also suppressed activity of the salmon Δ6 FAD promoter although this activity was likely mediated through sites other than Sp1, possibly similar to those recognised by NF-Y and SREBP transcription factors. |
author2 |
Institute of Aquaculture orcid:0000-0002-3155-0844 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Zheng, Xiaozhong Leaver, Michael Tocher, Douglas R |
author_facet |
Zheng, Xiaozhong Leaver, Michael Tocher, Douglas R |
author_sort |
Zheng, Xiaozhong |
title |
Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters |
title_short |
Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters |
title_full |
Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters |
title_fullStr |
Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters |
title_full_unstemmed |
Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters |
title_sort |
long-chain polyunsaturated fatty acid synthesis in fish: comparative analysis of atlantic salmon (salmo salar l.) and atlantic cod (gadus morhua l.) delta 6 fatty acyl desaturase gene promoters |
publisher |
Elsevier |
publishDate |
2009 |
url |
http://hdl.handle.net/1893/1707 https://doi.org/10.1016/j.cbpb.2009.06.010 http://www.sciencedirect.com/science/journal/10964959 http://dspace.stir.ac.uk/bitstream/1893/1707/1/Zheng%20et%20al%202009-2.pdf |
genre |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
op_relation |
Zheng X, Leaver M & Tocher DR (2009) Long-chain polyunsaturated fatty acid synthesis in fish: Comparative analysis of Atlantic salmon (Salmo salar L.) and Atlantic cod (Gadus morhua L.) Delta 6 fatty acyl desaturase gene promoters. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 154 (3), pp. 255-263. http://www.sciencedirect.com/science/journal/10964959; https://doi.org/10.1016/j.cbpb.2009.06.010 http://hdl.handle.net/1893/1707 doi:10.1016/j.cbpb.2009.06.010 http://www.sciencedirect.com/science/journal/10964959 WOS:000270767700001 2-s2.0-70149086891 835482 http://dspace.stir.ac.uk/bitstream/1893/1707/1/Zheng%20et%20al%202009-2.pdf |
op_rights |
Published in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology by Elsevier. |
op_doi |
https://doi.org/10.1016/j.cbpb.2009.06.010 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
154 |
container_issue |
3 |
container_start_page |
255 |
op_container_end_page |
263 |
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1766358132182745088 |