Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues
Dietary conjugated linoleic acid (CLA) affects fat deposition and lipid metabolism in mammals, including livestock. To determine CLA effects in Atlantic salmon (Salmo salar), a major farmed fish species, fish were fed for 12 weeks on diets containing fish oil or fish oil with 2% and 4% CLA supplemen...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | http://hdl.handle.net/1893/1595 https://doi.org/10.1016/j.cbpa.2006.06.034 http://www.sciencedirect.com/science/journal/10956433 http://dspace.stir.ac.uk/bitstream/1893/1595/1/LeaverCBP2006finpost.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/1595 2023-05-15T15:31:04+02:00 Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues Leaver, Michael Tocher, Douglas R Obach, Alex Jensen, Linda Henderson, R James Porter, Mark J R Krey, Grigorios Institute of Aquaculture Nutreco Aquaculture Research Centre AS University of Stirling National Agricultural Research Foundation orcid:0000-0002-3155-0844 orcid:0000-0002-8603-9410 2006-10 application/pdf http://hdl.handle.net/1893/1595 https://doi.org/10.1016/j.cbpa.2006.06.034 http://www.sciencedirect.com/science/journal/10956433 http://dspace.stir.ac.uk/bitstream/1893/1595/1/LeaverCBP2006finpost.pdf en eng Elsevier Leaver M, Tocher DR, Obach A, Jensen L, Henderson RJ, Porter MJR & Krey G (2006) Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 145 (2), pp. 258-267. http://www.sciencedirect.com/science/journal/10956433; https://doi.org/10.1016/j.cbpa.2006.06.034 http://hdl.handle.net/1893/1595 doi:10.1016/j.cbpa.2006.06.034 http://www.sciencedirect.com/science/journal/10956433 WOS:000241222500014 2-s2.0-33748634065 836241 http://dspace.stir.ac.uk/bitstream/1893/1595/1/LeaverCBP2006finpost.pdf Published in Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology by Elsevier. CLA PPAR Atlantic salmon desaturase beta-oxidation gene expression Dietary supplements Fishes Feeding and feeds Lipoproteins Fish Journal Article AM - Accepted Manuscript 2006 ftunivstirling https://doi.org/10.1016/j.cbpa.2006.06.034 2022-06-13T18:44:09Z Dietary conjugated linoleic acid (CLA) affects fat deposition and lipid metabolism in mammals, including livestock. To determine CLA effects in Atlantic salmon (Salmo salar), a major farmed fish species, fish were fed for 12 weeks on diets containing fish oil or fish oil with 2% and 4% CLA supplementation. Fatty acid composition of the tissues showed deposition of CLA with accumulation being 2 to 3 fold higher in muscle than in liver. CLA had no effect on feed conversion efficiency or growth of the fish but there was a decreased lipid content and increased protein content after 4%CLA feeding. Thus, the protein:lipid ratio in whole fish was increased in fish fed 4% CLA and triacylglycerol in liver was decreased. Liver β-oxidation was increased whilst both red muscle β-oxidation capacity and CPT1 activity was decreased by dietary CLA. Liver highly unsaturated fatty acid (HUFA) biosynthetic capacity was increased and the relative proportion of liver HUFA was marginally increased in salmon fed CLA. CLA had no effect on fatty acid Δ6 desaturase mRNA expression, but fatty acid elongase mRNA was increased in liver and intestine. In addition, the relative compositions of unsaturated and monounsaturated fatty acids changed after CLA feeding. CLA had no effect on PPARα or PPARγ expression in liver or intestine, although PPARβ2A expression was reduced in liver at 4% CLA feeding. CLA did not affect hepatic malic enzyme activity. Thus, overall, the effect of dietary CLA was to increase β-oxidation in liver, to reduce levels of total body lipid and liver triacylglycerol, and to affect liver fatty acid composition, with increased elongase expression and HUFA biosynthetic capacity. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 145 2 258 267 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
CLA PPAR Atlantic salmon desaturase beta-oxidation gene expression Dietary supplements Fishes Feeding and feeds Lipoproteins Fish |
spellingShingle |
CLA PPAR Atlantic salmon desaturase beta-oxidation gene expression Dietary supplements Fishes Feeding and feeds Lipoproteins Fish Leaver, Michael Tocher, Douglas R Obach, Alex Jensen, Linda Henderson, R James Porter, Mark J R Krey, Grigorios Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues |
topic_facet |
CLA PPAR Atlantic salmon desaturase beta-oxidation gene expression Dietary supplements Fishes Feeding and feeds Lipoproteins Fish |
description |
Dietary conjugated linoleic acid (CLA) affects fat deposition and lipid metabolism in mammals, including livestock. To determine CLA effects in Atlantic salmon (Salmo salar), a major farmed fish species, fish were fed for 12 weeks on diets containing fish oil or fish oil with 2% and 4% CLA supplementation. Fatty acid composition of the tissues showed deposition of CLA with accumulation being 2 to 3 fold higher in muscle than in liver. CLA had no effect on feed conversion efficiency or growth of the fish but there was a decreased lipid content and increased protein content after 4%CLA feeding. Thus, the protein:lipid ratio in whole fish was increased in fish fed 4% CLA and triacylglycerol in liver was decreased. Liver β-oxidation was increased whilst both red muscle β-oxidation capacity and CPT1 activity was decreased by dietary CLA. Liver highly unsaturated fatty acid (HUFA) biosynthetic capacity was increased and the relative proportion of liver HUFA was marginally increased in salmon fed CLA. CLA had no effect on fatty acid Δ6 desaturase mRNA expression, but fatty acid elongase mRNA was increased in liver and intestine. In addition, the relative compositions of unsaturated and monounsaturated fatty acids changed after CLA feeding. CLA had no effect on PPARα or PPARγ expression in liver or intestine, although PPARβ2A expression was reduced in liver at 4% CLA feeding. CLA did not affect hepatic malic enzyme activity. Thus, overall, the effect of dietary CLA was to increase β-oxidation in liver, to reduce levels of total body lipid and liver triacylglycerol, and to affect liver fatty acid composition, with increased elongase expression and HUFA biosynthetic capacity. |
author2 |
Institute of Aquaculture Nutreco Aquaculture Research Centre AS University of Stirling National Agricultural Research Foundation orcid:0000-0002-3155-0844 orcid:0000-0002-8603-9410 |
format |
Article in Journal/Newspaper |
author |
Leaver, Michael Tocher, Douglas R Obach, Alex Jensen, Linda Henderson, R James Porter, Mark J R Krey, Grigorios |
author_facet |
Leaver, Michael Tocher, Douglas R Obach, Alex Jensen, Linda Henderson, R James Porter, Mark J R Krey, Grigorios |
author_sort |
Leaver, Michael |
title |
Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues |
title_short |
Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues |
title_full |
Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues |
title_fullStr |
Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues |
title_full_unstemmed |
Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues |
title_sort |
effect of dietary conjugated linoleic acid (cla) on lipid composition, metabolism and gene expression in atlantic salmon (salmo salar) tissues |
publisher |
Elsevier |
publishDate |
2006 |
url |
http://hdl.handle.net/1893/1595 https://doi.org/10.1016/j.cbpa.2006.06.034 http://www.sciencedirect.com/science/journal/10956433 http://dspace.stir.ac.uk/bitstream/1893/1595/1/LeaverCBP2006finpost.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Leaver M, Tocher DR, Obach A, Jensen L, Henderson RJ, Porter MJR & Krey G (2006) Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 145 (2), pp. 258-267. http://www.sciencedirect.com/science/journal/10956433; https://doi.org/10.1016/j.cbpa.2006.06.034 http://hdl.handle.net/1893/1595 doi:10.1016/j.cbpa.2006.06.034 http://www.sciencedirect.com/science/journal/10956433 WOS:000241222500014 2-s2.0-33748634065 836241 http://dspace.stir.ac.uk/bitstream/1893/1595/1/LeaverCBP2006finpost.pdf |
op_rights |
Published in Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology by Elsevier. |
op_doi |
https://doi.org/10.1016/j.cbpa.2006.06.034 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
145 |
container_issue |
2 |
container_start_page |
258 |
op_container_end_page |
267 |
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1766361563576401920 |