Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L.
Vaccines are commonly used in salmonid aquaculture as a method of disease prevention. Although there is a substantial amount of published research regarding the immunological and physiological effects following the injection of different polyvalent vaccines and DNA vaccines, there are no published r...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2010
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Online Access: | http://hdl.handle.net/1893/15462 https://doi.org/10.1016/j.fsi.2009.12.017 http://dspace.stir.ac.uk/bitstream/1893/15462/1/Fish%20and%20Shellfish%20Immunology%20-%20Atlantic%20Salmon.pdf |
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ftunivstirling:oai:dspace.stir.ac.uk:1893/15462 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
DNA vaccine Adjuvant vaccine Innate immune response Lysozyme activity Adaptive immune response Neutralizing antibody Antibody titre Aeromonas salmonicida Listonella anguillarum Freshwater & Marine Ecology Fish & Wildlife Biology & Management Community & Population Ecology |
spellingShingle |
DNA vaccine Adjuvant vaccine Innate immune response Lysozyme activity Adaptive immune response Neutralizing antibody Antibody titre Aeromonas salmonicida Listonella anguillarum Freshwater & Marine Ecology Fish & Wildlife Biology & Management Community & Population Ecology Skinner, Lisa A LaPatra, Scott E Adams, Alexandra Thompson, Kimberly Balfry, Shannon K McKinley, R Scott Schulte, Patricia M Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. |
topic_facet |
DNA vaccine Adjuvant vaccine Innate immune response Lysozyme activity Adaptive immune response Neutralizing antibody Antibody titre Aeromonas salmonicida Listonella anguillarum Freshwater & Marine Ecology Fish & Wildlife Biology & Management Community & Population Ecology |
description |
Vaccines are commonly used in salmonid aquaculture as a method of disease prevention. Although there is a substantial amount of published research regarding the immunological and physiological effects following the injection of different polyvalent vaccines and DNA vaccines, there are no published reports examining the physiological and immunological effects of concurrent vaccine injection, which is the situation encountered in aquaculture. Using key immunological parameters such as lysozyme activity and specific antibody titres we examined the short-term activation of the immune response of cultured Atlantic salmon (Salmo salar L.) following concurrent injection with a traditional, polyvalent, oil-adjuvanted vaccine (AV) and an IHNV-specific DNA vaccine (DV). Our results indicate that different aspects of the innate and adaptive immune responses are influenced in either a positive or negative manner. While concurrent vaccine injection elicited an increase in lysozyme activity, changes in antibody titre (Ab) were antigen specific. The production of anti-Aeromonas salmonicida Abs was significantly greater in the combined vaccine group at 296 degree days post-vaccine injection (dd pvi), while the production of anti-Listonella anguillarum Abs was significantly greater at 106 dd pvi in the combined vaccine group. Of even greater interest was the apparent delay in production of IHNV-specific neutralizing antibodies (NAb) when the DV was injected concurrently with the polyvalent AV. The results indicated that concurrent injection of a polyvalent oil-AV and a DV can be beneficial to the production of antibodies; however, the specific anti-viral response may be delayed. |
author2 |
University of British Columbia Clear Springs Foods Inc Institute of Aquaculture Centre for Aquaculture and Environmental Research |
format |
Article in Journal/Newspaper |
author |
Skinner, Lisa A LaPatra, Scott E Adams, Alexandra Thompson, Kimberly Balfry, Shannon K McKinley, R Scott Schulte, Patricia M |
author_facet |
Skinner, Lisa A LaPatra, Scott E Adams, Alexandra Thompson, Kimberly Balfry, Shannon K McKinley, R Scott Schulte, Patricia M |
author_sort |
Skinner, Lisa A |
title |
Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. |
title_short |
Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. |
title_full |
Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. |
title_fullStr |
Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. |
title_full_unstemmed |
Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. |
title_sort |
concurrent injection of a rhabdovirus-specific dna vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in atlantic salmon, salmo salar l. |
publisher |
Elsevier |
publishDate |
2010 |
url |
http://hdl.handle.net/1893/15462 https://doi.org/10.1016/j.fsi.2009.12.017 http://dspace.stir.ac.uk/bitstream/1893/15462/1/Fish%20and%20Shellfish%20Immunology%20-%20Atlantic%20Salmon.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Skinner LA, LaPatra SE, Adams A, Thompson K, Balfry SK, McKinley RS & Schulte PM (2010) Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. Fish and Shellfish Immunology, 28 (4), pp. 579-586. https://doi.org/10.1016/j.fsi.2009.12.017 http://hdl.handle.net/1893/15462 doi:10.1016/j.fsi.2009.12.017 WOS:000276935700010 2-s2.0-77949905446 698465 http://dspace.stir.ac.uk/bitstream/1893/15462/1/Fish%20and%20Shellfish%20Immunology%20-%20Atlantic%20Salmon.pdf |
op_rights |
The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 2999-12-31 [Fish and Shellfish Immunology - Atlantic Salmon.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. |
op_doi |
https://doi.org/10.1016/j.fsi.2009.12.017 |
container_title |
Fish & Shellfish Immunology |
container_volume |
28 |
container_issue |
4 |
container_start_page |
579 |
op_container_end_page |
586 |
_version_ |
1766362368674103296 |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/15462 2023-05-15T15:31:52+02:00 Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. Skinner, Lisa A LaPatra, Scott E Adams, Alexandra Thompson, Kimberly Balfry, Shannon K McKinley, R Scott Schulte, Patricia M University of British Columbia Clear Springs Foods Inc Institute of Aquaculture Centre for Aquaculture and Environmental Research 2010-04 application/pdf http://hdl.handle.net/1893/15462 https://doi.org/10.1016/j.fsi.2009.12.017 http://dspace.stir.ac.uk/bitstream/1893/15462/1/Fish%20and%20Shellfish%20Immunology%20-%20Atlantic%20Salmon.pdf en eng Elsevier Skinner LA, LaPatra SE, Adams A, Thompson K, Balfry SK, McKinley RS & Schulte PM (2010) Concurrent injection of a rhabdovirus-specific DNA vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in Atlantic salmon, Salmo salar L. Fish and Shellfish Immunology, 28 (4), pp. 579-586. https://doi.org/10.1016/j.fsi.2009.12.017 http://hdl.handle.net/1893/15462 doi:10.1016/j.fsi.2009.12.017 WOS:000276935700010 2-s2.0-77949905446 698465 http://dspace.stir.ac.uk/bitstream/1893/15462/1/Fish%20and%20Shellfish%20Immunology%20-%20Atlantic%20Salmon.pdf The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. http://www.rioxx.net/licenses/under-embargo-all-rights-reserved 2999-12-31 [Fish and Shellfish Immunology - Atlantic Salmon.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. DNA vaccine Adjuvant vaccine Innate immune response Lysozyme activity Adaptive immune response Neutralizing antibody Antibody titre Aeromonas salmonicida Listonella anguillarum Freshwater & Marine Ecology Fish & Wildlife Biology & Management Community & Population Ecology Journal Article VoR - Version of Record 2010 ftunivstirling https://doi.org/10.1016/j.fsi.2009.12.017 2022-06-13T18:45:49Z Vaccines are commonly used in salmonid aquaculture as a method of disease prevention. Although there is a substantial amount of published research regarding the immunological and physiological effects following the injection of different polyvalent vaccines and DNA vaccines, there are no published reports examining the physiological and immunological effects of concurrent vaccine injection, which is the situation encountered in aquaculture. Using key immunological parameters such as lysozyme activity and specific antibody titres we examined the short-term activation of the immune response of cultured Atlantic salmon (Salmo salar L.) following concurrent injection with a traditional, polyvalent, oil-adjuvanted vaccine (AV) and an IHNV-specific DNA vaccine (DV). Our results indicate that different aspects of the innate and adaptive immune responses are influenced in either a positive or negative manner. While concurrent vaccine injection elicited an increase in lysozyme activity, changes in antibody titre (Ab) were antigen specific. The production of anti-Aeromonas salmonicida Abs was significantly greater in the combined vaccine group at 296 degree days post-vaccine injection (dd pvi), while the production of anti-Listonella anguillarum Abs was significantly greater at 106 dd pvi in the combined vaccine group. Of even greater interest was the apparent delay in production of IHNV-specific neutralizing antibodies (NAb) when the DV was injected concurrently with the polyvalent AV. The results indicated that concurrent injection of a polyvalent oil-AV and a DV can be beneficial to the production of antibodies; however, the specific anti-viral response may be delayed. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Fish & Shellfish Immunology 28 4 579 586 |