Alternative cell line for the isolation of salmonid alphavirus-1
Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aquaculture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland;...
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Consortium of universities and research institutions in Iceland
2009
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ftunivstirling:oai:dspace.stir.ac.uk:1893/15453 2023-05-15T15:31:07+02:00 Alternative cell line for the isolation of salmonid alphavirus-1 Herath, Tharangani Costa, Janina Z Thompson, Kimberly Adams, Alexandra Richards, Randolph Institute of Aquaculture University of Stirling 2009 application/pdf http://hdl.handle.net/1893/15453 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf http://dspace.stir.ac.uk/bitstream/1893/15453/1/ias%20alternative%20cell%20line.pdf en eng Consortium of universities and research institutions in Iceland Herath T, Costa JZ, Thompson K, Adams A & Richards R (2009) Alternative cell line for the isolation of salmonid alphavirus-1. Icelandic Agricultural Sciences, 22, pp. 19-27. http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf http://hdl.handle.net/1893/15453 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf WOS:000273154900003 698631 http://dspace.stir.ac.uk/bitstream/1893/15453/1/ias%20alternative%20cell%20line.pdf The publisher has granted permission for use of this work in this Repository. Published in Icelandic Agricultural Sciences, 22, pp.19-27, 2009 by a Consortium of universities and research institutions in Iceland: http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf Cell culture CHSE-214 CHH-1 PD SAV-1 SHK-1 Aquaculture Immune response Fishes Atlantic salmon Journal Article VoR - Version of Record 2009 ftunivstirling 2022-06-13T18:41:44Z Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aquaculture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland; several established cell lines have since been tested for viral growth, although the ability of these cell lines to support primary virus isolation has not being examined. In the present study, CHSE-214, Chum salmon heart -1 (CHH-1) and Salmon head kidney -1 (SHK-1) cell lines were evaluated for isolation of SAV-1 from kidney samples of experimentally infected Atlantic salmon (Salmo salar). The presence of infection in these samples was confirmed both by cell culture and reverse transcription polymerase chain reaction (RT-PCR). Homogenates of kidney from fish 3 days post-infection (p.i.) were inoculated onto the three cell lines and the development of a cytopathic effect (CPE) recorded. The CHH-1 cells produced a rapid CPE from Day 6 p.i., while the CHSE-214 cells showed the presence of a CPE from Day 10 p.i. In comparison, a CPE developed much later in the SHK-1 cells, from Day 20 p.i. The virus was successfully isolated on all three cell lines in subsequent passages, indicating that CHSE-214, CHH-1, and SHK-1 cells can be used for the isolation and culture of SAV-1. The CHH-1 cell line, however, has proven the most useful, since the CPE developed the quickest in this cell line. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Sav’ ENVELOPE(156.400,156.400,68.817,68.817) |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Cell culture CHSE-214 CHH-1 PD SAV-1 SHK-1 Aquaculture Immune response Fishes Atlantic salmon |
spellingShingle |
Cell culture CHSE-214 CHH-1 PD SAV-1 SHK-1 Aquaculture Immune response Fishes Atlantic salmon Herath, Tharangani Costa, Janina Z Thompson, Kimberly Adams, Alexandra Richards, Randolph Alternative cell line for the isolation of salmonid alphavirus-1 |
topic_facet |
Cell culture CHSE-214 CHH-1 PD SAV-1 SHK-1 Aquaculture Immune response Fishes Atlantic salmon |
description |
Salmonid alphavirus (SAV) has recently become an economically important pathogen in salmonid aquaculture in Europe. Subtype SAV-1 causes salmon pancreas disease (SPD) in Atlantic salmon in Scotland and Ireland, and was first isolated on Chinook salmon embryo-214 (CHSE-214) cells in 1995 in Ireland; several established cell lines have since been tested for viral growth, although the ability of these cell lines to support primary virus isolation has not being examined. In the present study, CHSE-214, Chum salmon heart -1 (CHH-1) and Salmon head kidney -1 (SHK-1) cell lines were evaluated for isolation of SAV-1 from kidney samples of experimentally infected Atlantic salmon (Salmo salar). The presence of infection in these samples was confirmed both by cell culture and reverse transcription polymerase chain reaction (RT-PCR). Homogenates of kidney from fish 3 days post-infection (p.i.) were inoculated onto the three cell lines and the development of a cytopathic effect (CPE) recorded. The CHH-1 cells produced a rapid CPE from Day 6 p.i., while the CHSE-214 cells showed the presence of a CPE from Day 10 p.i. In comparison, a CPE developed much later in the SHK-1 cells, from Day 20 p.i. The virus was successfully isolated on all three cell lines in subsequent passages, indicating that CHSE-214, CHH-1, and SHK-1 cells can be used for the isolation and culture of SAV-1. The CHH-1 cell line, however, has proven the most useful, since the CPE developed the quickest in this cell line. |
author2 |
Institute of Aquaculture University of Stirling |
format |
Article in Journal/Newspaper |
author |
Herath, Tharangani Costa, Janina Z Thompson, Kimberly Adams, Alexandra Richards, Randolph |
author_facet |
Herath, Tharangani Costa, Janina Z Thompson, Kimberly Adams, Alexandra Richards, Randolph |
author_sort |
Herath, Tharangani |
title |
Alternative cell line for the isolation of salmonid alphavirus-1 |
title_short |
Alternative cell line for the isolation of salmonid alphavirus-1 |
title_full |
Alternative cell line for the isolation of salmonid alphavirus-1 |
title_fullStr |
Alternative cell line for the isolation of salmonid alphavirus-1 |
title_full_unstemmed |
Alternative cell line for the isolation of salmonid alphavirus-1 |
title_sort |
alternative cell line for the isolation of salmonid alphavirus-1 |
publisher |
Consortium of universities and research institutions in Iceland |
publishDate |
2009 |
url |
http://hdl.handle.net/1893/15453 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf http://dspace.stir.ac.uk/bitstream/1893/15453/1/ias%20alternative%20cell%20line.pdf |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Herath T, Costa JZ, Thompson K, Adams A & Richards R (2009) Alternative cell line for the isolation of salmonid alphavirus-1. Icelandic Agricultural Sciences, 22, pp. 19-27. http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf http://hdl.handle.net/1893/15453 http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf WOS:000273154900003 698631 http://dspace.stir.ac.uk/bitstream/1893/15453/1/ias%20alternative%20cell%20line.pdf |
op_rights |
The publisher has granted permission for use of this work in this Repository. Published in Icelandic Agricultural Sciences, 22, pp.19-27, 2009 by a Consortium of universities and research institutions in Iceland: http://www.landbunadur.is/landbunadur/wgsamvef.nsf/8bbba2777ac88c4000256a89000a2ddb/93e7759f88de1e9400257672004b7e4d/$FILE/IAS%20Alternative%20cell%20line.pdf |
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1766361604312530944 |