Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar)
In homeothermic vertebrates inhabiting temperate latitudes, it is clear that the seasonal changes in daylength are decoded by the master circadian clock which through secondary messengers, like pineal melatonin secretion, entrains rhythmic physiology to local conditions. In contrast, the entrainment...
Published in: | Chronobiology International |
---|---|
Main Authors: | , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Taylor & Francis (Informa Healthcare) USA
2009
|
Subjects: | |
Online Access: | http://hdl.handle.net/1893/1165 https://doi.org/10.1080/07420520902820947 http://dspace.stir.ac.uk/bitstream/1893/1165/1/CBI_26%283%29_Davie_et_al.pdf |
id |
ftunivstirling:oai:dspace.stir.ac.uk:1893/1165 |
---|---|
record_format |
openpolar |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/1165 2023-05-15T15:28:31+02:00 Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) Davie, Andrew Minghetti, Matteo Migaud, Herve Institute of Aquaculture orcid:0000-0002-9524-618X orcid:0000-0002-5404-7512 2009-04 application/pdf http://hdl.handle.net/1893/1165 https://doi.org/10.1080/07420520902820947 http://dspace.stir.ac.uk/bitstream/1893/1165/1/CBI_26%283%29_Davie_et_al.pdf en eng Taylor & Francis (Informa Healthcare) USA Davie A, Minghetti M & Migaud H (2009) Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar). Chronobiology International, 26 (3), pp. 379-395. https://doi.org/10.1080/07420520902820947 http://hdl.handle.net/1893/1165 doi:10.1080/07420520902820947 WOS:000265291700001 2-s2.0-67651148046 836495 http://dspace.stir.ac.uk/bitstream/1893/1165/1/CBI_26%283%29_Davie_et_al.pdf Published in Chronobiology International. Copyright: Taylor & Francis (Informa Healthcare).; This is an electronic version of an article published in Chronobiology International, Volume 26, Issue 3, April 2009, pp. 379 - 395. Chronobiology International is available online at: http://www.informaworld.com/openurl?genre=article&issn=0742-0528&volume=26&issue=3&spage=379 2010-05-31 [CBI_26(3)_Davie_et_al.pdf] Publisher conditions require a 12 month embargo. Atlantic salmon Salmo salar Circadian clock Potoperiodism Clock Per 2 Photoperiodism Atlantic Salmon Behavior Journal Article AM - Accepted Manuscript 2009 ftunivstirling https://doi.org/10.1080/07420520902820947 2022-06-13T18:45:44Z In homeothermic vertebrates inhabiting temperate latitudes, it is clear that the seasonal changes in daylength are decoded by the master circadian clock which through secondary messengers, like pineal melatonin secretion, entrains rhythmic physiology to local conditions. In contrast, the entrainment and neuroendocrine regulation of rhythmic physiology in temperate teleosts is not as clear, primarily due to the lack of understanding of the clock gene system in these species. In this study we analyzed the diel expression of the clock genes in brains of Atlantic salmon, a species that is both highly photoperiodic and displays robust clock-controlled behavior. Atlantic salmon parr were acclimated to either long (LD) or short day (SD) photoperiods for one month and thereafter sampled at 4 h intervals over a 24 h cycle. Clock, Bmal1, per2, and cry2 were all actively expressed in salmon brain homogenates and, with the exception of per2, all displayed rhythmic expression under SD photoperiods that parallels that reported in zebrafish. Interestingly, daylength significantly altered the mRNA expression of all clock genes studied with Clock, Bmal1, and per2 all becoming arrhythmic under the LD compared to SD photoperiod, while cry2 expression was phase delayed under LD. It is thus proposed that the clock gene system is actively expressed in Atlantic salmon and, furthermore, as has been reported in homeothermic vertebrates, it appears that clock expression is daylength-dependent. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository Chronobiology International 26 3 379 395 |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Atlantic salmon Salmo salar Circadian clock Potoperiodism Clock Per 2 Photoperiodism Atlantic Salmon Behavior |
spellingShingle |
Atlantic salmon Salmo salar Circadian clock Potoperiodism Clock Per 2 Photoperiodism Atlantic Salmon Behavior Davie, Andrew Minghetti, Matteo Migaud, Herve Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) |
topic_facet |
Atlantic salmon Salmo salar Circadian clock Potoperiodism Clock Per 2 Photoperiodism Atlantic Salmon Behavior |
description |
In homeothermic vertebrates inhabiting temperate latitudes, it is clear that the seasonal changes in daylength are decoded by the master circadian clock which through secondary messengers, like pineal melatonin secretion, entrains rhythmic physiology to local conditions. In contrast, the entrainment and neuroendocrine regulation of rhythmic physiology in temperate teleosts is not as clear, primarily due to the lack of understanding of the clock gene system in these species. In this study we analyzed the diel expression of the clock genes in brains of Atlantic salmon, a species that is both highly photoperiodic and displays robust clock-controlled behavior. Atlantic salmon parr were acclimated to either long (LD) or short day (SD) photoperiods for one month and thereafter sampled at 4 h intervals over a 24 h cycle. Clock, Bmal1, per2, and cry2 were all actively expressed in salmon brain homogenates and, with the exception of per2, all displayed rhythmic expression under SD photoperiods that parallels that reported in zebrafish. Interestingly, daylength significantly altered the mRNA expression of all clock genes studied with Clock, Bmal1, and per2 all becoming arrhythmic under the LD compared to SD photoperiod, while cry2 expression was phase delayed under LD. It is thus proposed that the clock gene system is actively expressed in Atlantic salmon and, furthermore, as has been reported in homeothermic vertebrates, it appears that clock expression is daylength-dependent. |
author2 |
Institute of Aquaculture orcid:0000-0002-9524-618X orcid:0000-0002-5404-7512 |
format |
Article in Journal/Newspaper |
author |
Davie, Andrew Minghetti, Matteo Migaud, Herve |
author_facet |
Davie, Andrew Minghetti, Matteo Migaud, Herve |
author_sort |
Davie, Andrew |
title |
Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) |
title_short |
Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) |
title_full |
Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) |
title_fullStr |
Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar) |
title_sort |
seasonal variations in clock-gene expression in atlantic salmon (salmo salar) |
publisher |
Taylor & Francis (Informa Healthcare) USA |
publishDate |
2009 |
url |
http://hdl.handle.net/1893/1165 https://doi.org/10.1080/07420520902820947 http://dspace.stir.ac.uk/bitstream/1893/1165/1/CBI_26%283%29_Davie_et_al.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Davie A, Minghetti M & Migaud H (2009) Seasonal Variations in Clock-Gene Expression in Atlantic Salmon (Salmo salar). Chronobiology International, 26 (3), pp. 379-395. https://doi.org/10.1080/07420520902820947 http://hdl.handle.net/1893/1165 doi:10.1080/07420520902820947 WOS:000265291700001 2-s2.0-67651148046 836495 http://dspace.stir.ac.uk/bitstream/1893/1165/1/CBI_26%283%29_Davie_et_al.pdf |
op_rights |
Published in Chronobiology International. Copyright: Taylor & Francis (Informa Healthcare).; This is an electronic version of an article published in Chronobiology International, Volume 26, Issue 3, April 2009, pp. 379 - 395. Chronobiology International is available online at: http://www.informaworld.com/openurl?genre=article&issn=0742-0528&volume=26&issue=3&spage=379 2010-05-31 [CBI_26(3)_Davie_et_al.pdf] Publisher conditions require a 12 month embargo. |
op_doi |
https://doi.org/10.1080/07420520902820947 |
container_title |
Chronobiology International |
container_volume |
26 |
container_issue |
3 |
container_start_page |
379 |
op_container_end_page |
395 |
_version_ |
1766358877346988032 |