Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland
Genetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified...
Main Authors: | , , , , , , |
---|---|
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Naturalis
2000
|
Subjects: | |
Online Access: | http://hdl.handle.net/1893/10232 http://www.ctoz.nl/ctz/vol69/nr01/art10 http://dspace.stir.ac.uk/bitstream/1893/10232/1/Shinn%20et%20al%202000a%2018S%20sequencing.pdf |
id |
ftunivstirling:oai:dspace.stir.ac.uk:1893/10232 |
---|---|
record_format |
openpolar |
spelling |
ftunivstirling:oai:dspace.stir.ac.uk:1893/10232 2023-05-15T15:30:25+02:00 Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland Shinn, Andrew Banks, Bryony Tange, Nahoko Bron, James Sommerville, Christina Aoki, Takashi Wootten, Rodney Institute of Aquaculture University of Stirling Tokyo University of Fisheries orcid:0000-0002-5434-2685 orcid:0000-0003-3544-0519 2000 application/pdf http://hdl.handle.net/1893/10232 http://www.ctoz.nl/ctz/vol69/nr01/art10 http://dspace.stir.ac.uk/bitstream/1893/10232/1/Shinn%20et%20al%202000a%2018S%20sequencing.pdf en eng Naturalis Shinn A, Banks B, Tange N, Bron J, Sommerville C, Aoki T & Wootten R (2000) Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland. Contributions to Zoology, 69 (1/2), pp. 89-98. http://www.ctoz.nl/ctz/vol69/nr01/art10 http://hdl.handle.net/1893/10232 http://www.ctoz.nl/ctz/vol69/nr01/art10 2-s2.0-0034354424 756340 http://dspace.stir.ac.uk/bitstream/1893/10232/1/Shinn%20et%20al%202000a%2018S%20sequencing.pdf Publisher is open-access. Open access publishing allows free access to and distribution of published articles where the author retains copyright of their work by employing a Creative Commons attribution 3.0 License. Proper attribution of authorship and correct citation details should be given. http://creativecommons.org/licenses/by/3.0/ CC-BY Sea louse Lepeophtheirus salmonis copepod Crustacea Atlantic salmon sequencing 18S ribosomal DNA internal transcribed spacer population genetics Journal Article VoR - Version of Record 2000 ftunivstirling 2022-06-13T18:41:46Z Genetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified by the polymerase chain reaction (PCR). Lice were collected from wild and farmed Atlantic salmon (Salmo salar L., 1758) from nine selected localities around the Scottish coastline. A 0.9kb fragment of the 18S ribosomal RNA gene was amplified and compared for several samples of lice which showed no observable differences between the lice from different collection sites confirming the absence of cryptic species. The 454 nucleotide ITS-1 sequence showed differences between derived sequences from 13 sea lice samples from 4 collection sites which included 2 farm sites and 2 sites where lice were taken from wild fish. Across all samples, there was a 92.14% similarity in the ITS-1 sequence. The percentage similarity in the ITS-1 sequence in samples of lice from two fish farms were 99.71% (site A) and 95.72% (site D) but only 86.90% (site B) and 86.03% (site C) similarity was shown in lice samples taken from sites where wild salmonids were caught. The greater similarity between the ITS-1 sequence within farm sites may be attributed to a restricted gene flow within lice populations in Atlantic salmon cage sites. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Stirling: Stirling Digital Research Repository |
institution |
Open Polar |
collection |
University of Stirling: Stirling Digital Research Repository |
op_collection_id |
ftunivstirling |
language |
English |
topic |
Sea louse Lepeophtheirus salmonis copepod Crustacea Atlantic salmon sequencing 18S ribosomal DNA internal transcribed spacer population genetics |
spellingShingle |
Sea louse Lepeophtheirus salmonis copepod Crustacea Atlantic salmon sequencing 18S ribosomal DNA internal transcribed spacer population genetics Shinn, Andrew Banks, Bryony Tange, Nahoko Bron, James Sommerville, Christina Aoki, Takashi Wootten, Rodney Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland |
topic_facet |
Sea louse Lepeophtheirus salmonis copepod Crustacea Atlantic salmon sequencing 18S ribosomal DNA internal transcribed spacer population genetics |
description |
Genetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified by the polymerase chain reaction (PCR). Lice were collected from wild and farmed Atlantic salmon (Salmo salar L., 1758) from nine selected localities around the Scottish coastline. A 0.9kb fragment of the 18S ribosomal RNA gene was amplified and compared for several samples of lice which showed no observable differences between the lice from different collection sites confirming the absence of cryptic species. The 454 nucleotide ITS-1 sequence showed differences between derived sequences from 13 sea lice samples from 4 collection sites which included 2 farm sites and 2 sites where lice were taken from wild fish. Across all samples, there was a 92.14% similarity in the ITS-1 sequence. The percentage similarity in the ITS-1 sequence in samples of lice from two fish farms were 99.71% (site A) and 95.72% (site D) but only 86.90% (site B) and 86.03% (site C) similarity was shown in lice samples taken from sites where wild salmonids were caught. The greater similarity between the ITS-1 sequence within farm sites may be attributed to a restricted gene flow within lice populations in Atlantic salmon cage sites. |
author2 |
Institute of Aquaculture University of Stirling Tokyo University of Fisheries orcid:0000-0002-5434-2685 orcid:0000-0003-3544-0519 |
format |
Article in Journal/Newspaper |
author |
Shinn, Andrew Banks, Bryony Tange, Nahoko Bron, James Sommerville, Christina Aoki, Takashi Wootten, Rodney |
author_facet |
Shinn, Andrew Banks, Bryony Tange, Nahoko Bron, James Sommerville, Christina Aoki, Takashi Wootten, Rodney |
author_sort |
Shinn, Andrew |
title |
Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland |
title_short |
Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland |
title_full |
Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland |
title_fullStr |
Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland |
title_full_unstemmed |
Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland |
title_sort |
utility of 18s rdna and its sequences as population markers for lepeophtheirus salmonis (copepoda: caligidae) parasitising atlantic salmon (salmo salar) in scotland |
publisher |
Naturalis |
publishDate |
2000 |
url |
http://hdl.handle.net/1893/10232 http://www.ctoz.nl/ctz/vol69/nr01/art10 http://dspace.stir.ac.uk/bitstream/1893/10232/1/Shinn%20et%20al%202000a%2018S%20sequencing.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Shinn A, Banks B, Tange N, Bron J, Sommerville C, Aoki T & Wootten R (2000) Utility of 18S rDNA and ITS sequences as population markers for Lepeophtheirus salmonis (Copepoda: Caligidae) parasitising Atlantic salmon (Salmo salar) in Scotland. Contributions to Zoology, 69 (1/2), pp. 89-98. http://www.ctoz.nl/ctz/vol69/nr01/art10 http://hdl.handle.net/1893/10232 http://www.ctoz.nl/ctz/vol69/nr01/art10 2-s2.0-0034354424 756340 http://dspace.stir.ac.uk/bitstream/1893/10232/1/Shinn%20et%20al%202000a%2018S%20sequencing.pdf |
op_rights |
Publisher is open-access. Open access publishing allows free access to and distribution of published articles where the author retains copyright of their work by employing a Creative Commons attribution 3.0 License. Proper attribution of authorship and correct citation details should be given. http://creativecommons.org/licenses/by/3.0/ |
op_rightsnorm |
CC-BY |
_version_ |
1766360855185719296 |