Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry
Mixotrophic phytoplankton are an important component of the microbial communities of the open ocean surface. They can control the metabolic balance and biogeochemical cycling of biogenic materials. To identify environmental factors that control the ecological prosperity of mixotrophs over obligate a...
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ftunivsthongkong:oai:repository.ust.hk:1783.1-96704 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
The Hong Kong University of Science and Technology: HKUST Institutional Repository |
op_collection_id |
ftunivsthongkong |
language |
English |
topic |
Flow cytometry LysoTracker Green Mixotrophy Nanoeukaryotes Phosphorus Picoeukaryotes |
spellingShingle |
Flow cytometry LysoTracker Green Mixotrophy Nanoeukaryotes Phosphorus Picoeukaryotes Sato, Mitsuhide LIFS Hashihama, Fuminori Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry |
topic_facet |
Flow cytometry LysoTracker Green Mixotrophy Nanoeukaryotes Phosphorus Picoeukaryotes |
description |
Mixotrophic phytoplankton are an important component of the microbial communities of the open ocean surface. They can control the metabolic balance and biogeochemical cycling of biogenic materials. To identify environmental factors that control the ecological prosperity of mixotrophs over obligate autotrophs in the open ocean, we quantified potential mixotrophic picoand nanophytoplankton throughout a wide area of the North Pacific Ocean on latitudinal and longitudinal transects, using a combination of flow cytometry and the fluorescent probe LysoTracker Green. The proportions of potential mixotrophic pico- and nanophytoplankton were higher in the subtropical gyre and Bering Sea than in surrounding areas, and even higher in the western area of the subtropical gyre. When the proportion of potential mixotrophs within the surface mixed layer was regressed against various environmental parameters, soluble reactive phosphorus concentration showed a significant negative partial regression coefficient. This result reflected the east-west gradient in the subtropical gyre and is consistent with previous implications that low nutrient availability can facilitate phagotrophy by mixotrophic phytoplankton. Unexpectedly, water stability did not show a significant correlation with the proportion of potential mixotrophs, which suggests that instantaneous physical parameters do not significantly affect their nutritional mode. When the data sets from water below the mixed layer were included in the multiple regression analysis, the significant regression against soluble reactive phosphorus concentration disappeared. This result may suggest a complex response of phytoplankton in the deeper ocean environment. The present results reinforce the importance of phosphorus availability as a control factor of phytoplankton nutritional physiology in the subtropical North Pacific Ocean. © 2019 Inter-Research. |
format |
Article in Journal/Newspaper |
author |
Sato, Mitsuhide LIFS Hashihama, Fuminori |
author_facet |
Sato, Mitsuhide LIFS Hashihama, Fuminori |
author_sort |
Sato, Mitsuhide LIFS |
title |
Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry |
title_short |
Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry |
title_full |
Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry |
title_fullStr |
Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry |
title_full_unstemmed |
Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry |
title_sort |
assessment of potential phagotrophy by pico- and nanophytoplankton in the north pacific ocean using flow cytometry |
publishDate |
2019 |
url |
http://repository.ust.hk/ir/Record/1783.1-96704 https://doi.org/10.3354/ame01892 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0948-3055&rft.volume=v. 82&rft.issue=(3)&rft.date=2019&rft.spage=275&rft.aulast=Sato&rft.aufirst=M.&rft.atitle=Assessment%20of%20potential%20phagotrophy%20by%20pico-%20and%20nanophytoplankton%20in%20the%20North%20Pacific%20Ocean%20using%20flow%20cytometry&rft.title=Aquatic%20Microbial%20Ecology http://www.scopus.com/record/display.url?eid=2-s2.0-85063576648&origin=inward http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000487114000005 |
geographic |
Bering Sea Pacific |
geographic_facet |
Bering Sea Pacific |
genre |
Bering Sea |
genre_facet |
Bering Sea |
op_relation |
http://repository.ust.hk/ir/Record/1783.1-96704 Aquatic Microbial Ecology, v. 82, (3), 2019, p. 275-288 0948-3055 https://doi.org/10.3354/ame01892 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0948-3055&rft.volume=v. 82&rft.issue=(3)&rft.date=2019&rft.spage=275&rft.aulast=Sato&rft.aufirst=M.&rft.atitle=Assessment%20of%20potential%20phagotrophy%20by%20pico-%20and%20nanophytoplankton%20in%20the%20North%20Pacific%20Ocean%20using%20flow%20cytometry&rft.title=Aquatic%20Microbial%20Ecology http://www.scopus.com/record/display.url?eid=2-s2.0-85063576648&origin=inward http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000487114000005 |
op_doi |
https://doi.org/10.3354/ame01892 |
container_title |
Aquatic Microbial Ecology |
container_volume |
82 |
container_issue |
3 |
container_start_page |
275 |
op_container_end_page |
288 |
_version_ |
1766378165017509888 |
spelling |
ftunivsthongkong:oai:repository.ust.hk:1783.1-96704 2023-05-15T15:43:57+02:00 Assessment of potential phagotrophy by pico- and nanophytoplankton in the North Pacific Ocean using flow cytometry Sato, Mitsuhide LIFS Hashihama, Fuminori 2019 http://repository.ust.hk/ir/Record/1783.1-96704 https://doi.org/10.3354/ame01892 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0948-3055&rft.volume=v. 82&rft.issue=(3)&rft.date=2019&rft.spage=275&rft.aulast=Sato&rft.aufirst=M.&rft.atitle=Assessment%20of%20potential%20phagotrophy%20by%20pico-%20and%20nanophytoplankton%20in%20the%20North%20Pacific%20Ocean%20using%20flow%20cytometry&rft.title=Aquatic%20Microbial%20Ecology http://www.scopus.com/record/display.url?eid=2-s2.0-85063576648&origin=inward http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000487114000005 English eng http://repository.ust.hk/ir/Record/1783.1-96704 Aquatic Microbial Ecology, v. 82, (3), 2019, p. 275-288 0948-3055 https://doi.org/10.3354/ame01892 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0948-3055&rft.volume=v. 82&rft.issue=(3)&rft.date=2019&rft.spage=275&rft.aulast=Sato&rft.aufirst=M.&rft.atitle=Assessment%20of%20potential%20phagotrophy%20by%20pico-%20and%20nanophytoplankton%20in%20the%20North%20Pacific%20Ocean%20using%20flow%20cytometry&rft.title=Aquatic%20Microbial%20Ecology http://www.scopus.com/record/display.url?eid=2-s2.0-85063576648&origin=inward http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000487114000005 Flow cytometry LysoTracker Green Mixotrophy Nanoeukaryotes Phosphorus Picoeukaryotes Article 2019 ftunivsthongkong https://doi.org/10.3354/ame01892 2019-11-15T00:59:11Z Mixotrophic phytoplankton are an important component of the microbial communities of the open ocean surface. They can control the metabolic balance and biogeochemical cycling of biogenic materials. To identify environmental factors that control the ecological prosperity of mixotrophs over obligate autotrophs in the open ocean, we quantified potential mixotrophic picoand nanophytoplankton throughout a wide area of the North Pacific Ocean on latitudinal and longitudinal transects, using a combination of flow cytometry and the fluorescent probe LysoTracker Green. The proportions of potential mixotrophic pico- and nanophytoplankton were higher in the subtropical gyre and Bering Sea than in surrounding areas, and even higher in the western area of the subtropical gyre. When the proportion of potential mixotrophs within the surface mixed layer was regressed against various environmental parameters, soluble reactive phosphorus concentration showed a significant negative partial regression coefficient. This result reflected the east-west gradient in the subtropical gyre and is consistent with previous implications that low nutrient availability can facilitate phagotrophy by mixotrophic phytoplankton. Unexpectedly, water stability did not show a significant correlation with the proportion of potential mixotrophs, which suggests that instantaneous physical parameters do not significantly affect their nutritional mode. When the data sets from water below the mixed layer were included in the multiple regression analysis, the significant regression against soluble reactive phosphorus concentration disappeared. This result may suggest a complex response of phytoplankton in the deeper ocean environment. The present results reinforce the importance of phosphorus availability as a control factor of phytoplankton nutritional physiology in the subtropical North Pacific Ocean. © 2019 Inter-Research. Article in Journal/Newspaper Bering Sea The Hong Kong University of Science and Technology: HKUST Institutional Repository Bering Sea Pacific Aquatic Microbial Ecology 82 3 275 288 |