Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis
A newly designed Cytophaga-Flavobacteria-specific 16S rRNA gene primer pair was employed to investigate the CF community structure in the Bering Sea, revealing a previously unknown and unexpected high CF diversity in this high latitude cold sea. In total, 56 clones were sequenced and 50 unique CF 16...
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Online Access: | http://repository.ust.hk/ir/Record/1783.1-28677 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1017-7825&rft.volume=18&rft.issue=2&rft.date=2008&rft.spage=194&rft.aulast=Chen&rft.aufirst=Xihan&rft.atitle=Characterization+of+Cytophaga-Flavobacteria+community+structure+in+the+Bering+Sea+by+cluster-specific+16S+rRNA+gene+amplification+analysis&rft.title=Journal+of+microbiology+and+biotechnology |
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ftunivsthongkong:oai:repository.ust.hk:1783.1-28677 2023-05-15T15:43:10+02:00 Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis Chen, Xihan Zeng, Yonghui Jiao, Nianzhi 2008 http://repository.ust.hk/ir/Record/1783.1-28677 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1017-7825&rft.volume=18&rft.issue=2&rft.date=2008&rft.spage=194&rft.aulast=Chen&rft.aufirst=Xihan&rft.atitle=Characterization+of+Cytophaga-Flavobacteria+community+structure+in+the+Bering+Sea+by+cluster-specific+16S+rRNA+gene+amplification+analysis&rft.title=Journal+of+microbiology+and+biotechnology English eng http://repository.ust.hk/ir/Record/1783.1-28677 Journal of microbiology and biotechnology, v. 18, (2), 2008, Feb, p. 194-198 1017-7825 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1017-7825&rft.volume=18&rft.issue=2&rft.date=2008&rft.spage=194&rft.aulast=Chen&rft.aufirst=Xihan&rft.atitle=Characterization+of+Cytophaga-Flavobacteria+community+structure+in+the+Bering+Sea+by+cluster-specific+16S+rRNA+gene+amplification+analysis&rft.title=Journal+of+microbiology+and+biotechnology Bacteroidetes: classification Bacteroidetes: genetics Bacteroidetes: isolation & purification DNA Bacterial: genetics Ribosomal: genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA Ribosomal 16S: genetics Seawater: microbiology Sequence Analysis Article 2008 ftunivsthongkong 2019-09-03T18:12:25Z A newly designed Cytophaga-Flavobacteria-specific 16S rRNA gene primer pair was employed to investigate the CF community structure in the Bering Sea, revealing a previously unknown and unexpected high CF diversity in this high latitude cold sea. In total, 56 clones were sequenced and 50 unique CF 16 rRNA gene fragments were obtained, clustering into 16 CF subgroups, including nine cosmopolitan subgroups, five psychrophilic subgroups, and two putatively autochthonous subgroups. The majority of sequences (82%) were closely related to uncultured CF species and could not be classified into known CF genera, indicating the presence of a large number of so-far uncultivated CF species in the Bering Sea. Article in Journal/Newspaper Bering Sea The Hong Kong University of Science and Technology: HKUST Institutional Repository Bering Sea |
institution |
Open Polar |
collection |
The Hong Kong University of Science and Technology: HKUST Institutional Repository |
op_collection_id |
ftunivsthongkong |
language |
English |
topic |
Bacteroidetes: classification Bacteroidetes: genetics Bacteroidetes: isolation & purification DNA Bacterial: genetics Ribosomal: genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA Ribosomal 16S: genetics Seawater: microbiology Sequence Analysis |
spellingShingle |
Bacteroidetes: classification Bacteroidetes: genetics Bacteroidetes: isolation & purification DNA Bacterial: genetics Ribosomal: genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA Ribosomal 16S: genetics Seawater: microbiology Sequence Analysis Chen, Xihan Zeng, Yonghui Jiao, Nianzhi Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis |
topic_facet |
Bacteroidetes: classification Bacteroidetes: genetics Bacteroidetes: isolation & purification DNA Bacterial: genetics Ribosomal: genetics Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA Ribosomal 16S: genetics Seawater: microbiology Sequence Analysis |
description |
A newly designed Cytophaga-Flavobacteria-specific 16S rRNA gene primer pair was employed to investigate the CF community structure in the Bering Sea, revealing a previously unknown and unexpected high CF diversity in this high latitude cold sea. In total, 56 clones were sequenced and 50 unique CF 16 rRNA gene fragments were obtained, clustering into 16 CF subgroups, including nine cosmopolitan subgroups, five psychrophilic subgroups, and two putatively autochthonous subgroups. The majority of sequences (82%) were closely related to uncultured CF species and could not be classified into known CF genera, indicating the presence of a large number of so-far uncultivated CF species in the Bering Sea. |
format |
Article in Journal/Newspaper |
author |
Chen, Xihan Zeng, Yonghui Jiao, Nianzhi |
author_facet |
Chen, Xihan Zeng, Yonghui Jiao, Nianzhi |
author_sort |
Chen, Xihan |
title |
Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis |
title_short |
Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis |
title_full |
Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis |
title_fullStr |
Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis |
title_full_unstemmed |
Characterization of Cytophaga-Flavobacteria community structure in the Bering Sea by cluster-specific 16S rRNA gene amplification analysis |
title_sort |
characterization of cytophaga-flavobacteria community structure in the bering sea by cluster-specific 16s rrna gene amplification analysis |
publishDate |
2008 |
url |
http://repository.ust.hk/ir/Record/1783.1-28677 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1017-7825&rft.volume=18&rft.issue=2&rft.date=2008&rft.spage=194&rft.aulast=Chen&rft.aufirst=Xihan&rft.atitle=Characterization+of+Cytophaga-Flavobacteria+community+structure+in+the+Bering+Sea+by+cluster-specific+16S+rRNA+gene+amplification+analysis&rft.title=Journal+of+microbiology+and+biotechnology |
geographic |
Bering Sea |
geographic_facet |
Bering Sea |
genre |
Bering Sea |
genre_facet |
Bering Sea |
op_relation |
http://repository.ust.hk/ir/Record/1783.1-28677 Journal of microbiology and biotechnology, v. 18, (2), 2008, Feb, p. 194-198 1017-7825 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1017-7825&rft.volume=18&rft.issue=2&rft.date=2008&rft.spage=194&rft.aulast=Chen&rft.aufirst=Xihan&rft.atitle=Characterization+of+Cytophaga-Flavobacteria+community+structure+in+the+Bering+Sea+by+cluster-specific+16S+rRNA+gene+amplification+analysis&rft.title=Journal+of+microbiology+and+biotechnology |
_version_ |
1766377202865143808 |