Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice
The influenza A nucleoprotein (NP) is an attractive target for avian flu vaccine development because of its high conversancy in the evolutionary chain of the virus. Here we identified two novel HLA-A{*}0201 restricted NP epitopes, named H5N1 NP373-381 AMDSNTLEL (NP373) and NP458-466 FQGRGVFEL (NP458...
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ftunivsthongkong:oai:repository.ust.hk:1783.1-17239 2023-05-15T15:34:18+02:00 Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice Cheung, Ying-Kit Cheng, Samuel Chak-Sum Ke, Yan Xie, Yong 2010 http://repository.ust.hk/ir/Record/1783.1-17239 https://doi.org/10.1051/vetres/2009071 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0928-4249&rft.volume=41&rft.issue=2&rft.date=2010&rft.spage=&rft.aulast=Cheung&rft.aufirst=Ying-Kit&rft.atitle=Two+novel+HLA-A{*}0201+T-cell+epitopes+in+avian+H5N1+viral+nucleoprotein+induced+specific+immune+responses+in+HHD+mice http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000274934800008 http://www.scopus.com/record/display.url?eid=2-s2.0-77249148325&origin=inward English eng http://repository.ust.hk/ir/Record/1783.1-17239 Veterinary research, v. 41, (2), 2010, MAR-APR 0928-4249 https://doi.org/10.1051/vetres/2009071 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0928-4249&rft.volume=41&rft.issue=2&rft.date=2010&rft.spage=&rft.aulast=Cheung&rft.aufirst=Ying-Kit&rft.atitle=Two+novel+HLA-A{*}0201+T-cell+epitopes+in+avian+H5N1+viral+nucleoprotein+induced+specific+immune+responses+in+HHD+mice http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000274934800008 http://www.scopus.com/record/display.url?eid=2-s2.0-77249148325&origin=inward Influenza A virus H5N1 nucleoprotein HLA-A{*}0201 T-cell epitope Single-chain trimer HHD transgenic mouse model 0201 T-cell epitope HLA-A Article 2010 ftunivsthongkong https://doi.org/10.1051/vetres/2009071 2019-09-03T17:53:53Z The influenza A nucleoprotein (NP) is an attractive target for avian flu vaccine development because of its high conversancy in the evolutionary chain of the virus. Here we identified two novel HLA-A{*}0201 restricted NP epitopes, named H5N1 NP373-381 AMDSNTLEL (NP373) and NP458-466 FQGRGVFEL (NP458), using computational bioinformatic analysis. The NP peptides showed a high binding affinity to HLA-A{*}0201 on T2 cells, and were able to induce the activation of the cytotoxic T cells in the human peripheral blood mononuclear cells. We examined the potential of using NP373 and NP458 peptide sequences supplemented with a single-chain trimer as potential DNA vaccine candidates in an HHD transgenic mouse model. A gene gun delivery system was used for administrating the vaccine candidates into the animals. The results from cytotoxicity and ELISPOT assays indicated that a significant amount of IFN-gamma was secreted by the T cells of the vaccinated mice, and the T cells were able to eliminate the corresponding peptide-loaded T2 cells. The discovery of these novel immunogenic NP peptides provides valuable information for avian flu vaccine design and construction. Article in Journal/Newspaper Avian flu The Hong Kong University of Science and Technology: HKUST Institutional Repository Veterinary Research 41 2 24 |
institution |
Open Polar |
collection |
The Hong Kong University of Science and Technology: HKUST Institutional Repository |
op_collection_id |
ftunivsthongkong |
language |
English |
topic |
Influenza A virus H5N1 nucleoprotein HLA-A{*}0201 T-cell epitope Single-chain trimer HHD transgenic mouse model 0201 T-cell epitope HLA-A |
spellingShingle |
Influenza A virus H5N1 nucleoprotein HLA-A{*}0201 T-cell epitope Single-chain trimer HHD transgenic mouse model 0201 T-cell epitope HLA-A Cheung, Ying-Kit Cheng, Samuel Chak-Sum Ke, Yan Xie, Yong Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice |
topic_facet |
Influenza A virus H5N1 nucleoprotein HLA-A{*}0201 T-cell epitope Single-chain trimer HHD transgenic mouse model 0201 T-cell epitope HLA-A |
description |
The influenza A nucleoprotein (NP) is an attractive target for avian flu vaccine development because of its high conversancy in the evolutionary chain of the virus. Here we identified two novel HLA-A{*}0201 restricted NP epitopes, named H5N1 NP373-381 AMDSNTLEL (NP373) and NP458-466 FQGRGVFEL (NP458), using computational bioinformatic analysis. The NP peptides showed a high binding affinity to HLA-A{*}0201 on T2 cells, and were able to induce the activation of the cytotoxic T cells in the human peripheral blood mononuclear cells. We examined the potential of using NP373 and NP458 peptide sequences supplemented with a single-chain trimer as potential DNA vaccine candidates in an HHD transgenic mouse model. A gene gun delivery system was used for administrating the vaccine candidates into the animals. The results from cytotoxicity and ELISPOT assays indicated that a significant amount of IFN-gamma was secreted by the T cells of the vaccinated mice, and the T cells were able to eliminate the corresponding peptide-loaded T2 cells. The discovery of these novel immunogenic NP peptides provides valuable information for avian flu vaccine design and construction. |
format |
Article in Journal/Newspaper |
author |
Cheung, Ying-Kit Cheng, Samuel Chak-Sum Ke, Yan Xie, Yong |
author_facet |
Cheung, Ying-Kit Cheng, Samuel Chak-Sum Ke, Yan Xie, Yong |
author_sort |
Cheung, Ying-Kit |
title |
Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice |
title_short |
Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice |
title_full |
Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice |
title_fullStr |
Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice |
title_full_unstemmed |
Two novel HLA-A{*}0201 T-cell epitopes in avian H5N1 viral nucleoprotein induced specific immune responses in HHD mice |
title_sort |
two novel hla-a{*}0201 t-cell epitopes in avian h5n1 viral nucleoprotein induced specific immune responses in hhd mice |
publishDate |
2010 |
url |
http://repository.ust.hk/ir/Record/1783.1-17239 https://doi.org/10.1051/vetres/2009071 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0928-4249&rft.volume=41&rft.issue=2&rft.date=2010&rft.spage=&rft.aulast=Cheung&rft.aufirst=Ying-Kit&rft.atitle=Two+novel+HLA-A{*}0201+T-cell+epitopes+in+avian+H5N1+viral+nucleoprotein+induced+specific+immune+responses+in+HHD+mice http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000274934800008 http://www.scopus.com/record/display.url?eid=2-s2.0-77249148325&origin=inward |
genre |
Avian flu |
genre_facet |
Avian flu |
op_relation |
http://repository.ust.hk/ir/Record/1783.1-17239 Veterinary research, v. 41, (2), 2010, MAR-APR 0928-4249 https://doi.org/10.1051/vetres/2009071 http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=0928-4249&rft.volume=41&rft.issue=2&rft.date=2010&rft.spage=&rft.aulast=Cheung&rft.aufirst=Ying-Kit&rft.atitle=Two+novel+HLA-A{*}0201+T-cell+epitopes+in+avian+H5N1+viral+nucleoprotein+induced+specific+immune+responses+in+HHD+mice http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000274934800008 http://www.scopus.com/record/display.url?eid=2-s2.0-77249148325&origin=inward |
op_doi |
https://doi.org/10.1051/vetres/2009071 |
container_title |
Veterinary Research |
container_volume |
41 |
container_issue |
2 |
container_start_page |
24 |
_version_ |
1766364749647314944 |