Bacterial diversity in Arctic marine sediment determined by culture-dependent and -independent approaches

• Main Authors: Lin, Xuezheng, Wang, Zhen, Che, Shuai, Song, Weizhi, Yi, Dan
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2014
• Subjects: Arctic; Arctic Ocean; Advances in Polar Science; Polar Science
• Online Access: https://repository.hkust.edu.hk/ir/Record/1783.1-133122; https://doi.org/10.13679/j.advps.2014.1.00046

Bacterial diversity in surface sediment from the Arctic Ocean was investigated by culture-dependent and -independent approaches. Conventional culture-dependent techniques revealed 11 strains based on their distinct morphological characteristics on marine Zobell 2216E agar plates. Phylogenetic analysis showed that these isolates belonged to three major lineages of the Bacteria, γ-proteobacteria, Bacteroidetes and Actinobacteria, and that they included 10 genera. Most isolates were psychrotrophic, and NaCl was not necessary for their growth. Furthermore, they exhibited activity of at least one extracellular hydrolytic enzyme at 4°C and had various abilities to assimilate carbon sources. A total of 67 phylotypes were detected among 142 clones based on the 16S rRNA library of the total community DNA and grouped into nine major lineages of bacteria. Phylotypes affiliated with γ-, δ- and ε-proteobacteria accounted for 36.7%, 21.8% and 16.9% of the total clones, respectively. The rest of the clones belonged to Bacteroidetes, α-proteobacteria, Actinobacteria, Fusobacteria, Nitrospirae and an unclassified group.