Mameliella Sediminis Sp. Nov., a Novel Polyhydroxyalkanoate-Accumulating Bacterium

• Published in: International Journal of Systematic and Evolutionary Microbiology
• Main Authors: Zheng, Wei-shuang, Zhai, Sheng-qiang, Zhang, Meng-jun, Huang, Yi
• Format: Article in Journal/Newspaper
• Language: English
• Published: Microbiology Society 2022
• Subjects: ANI; DDDH; Draft genome sequencing; Mameliella; Polyhydroxyalkanoate; Antarc*
• Online Access: https://repository.hkust.edu.hk/ir/Record/1783.1-116676; https://doi.org/10.1099/ijsem.0.005274; http://lbdiscover.ust.hk/uresolver?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=1466-5034&rft.volume=72&rft.issue=(3)&rft.date=2022&rft.spage=&rft.aulast=Zheng&rft.aufirst=&rft.atitle=Mameliella+sediminis+sp.+nov.,+a+novel+polyhydroxyalkanoate-accumulating+bacterium&rft.title=International+journal+of+systematic+and+evolutionary+microbiology; http://www.scopus.com/record/display.url?eid=2-s2.0-85126079681&origin=inward; http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000785384900010

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterium, capable of producing poly-β-hydroxyalkanoate, designated DP3N28-2T, was isolated from the sediment collected from Daya Bay, Guangdong, PR China. Optimal growth occurred at 37-40 °C, pH 6.0 and in the presence of 4 % NaCl. The 16S rRNA gene sequences analysis revealed that DP3N28-2T showed highest similarities with Mameliella alba DSM 23384T (98.3 %), Antarctobacter jejuensis 13-2-B6T (97.2 %), Antarctobacter heliothermus El-219T (96.8 %), Maliponia aquimaris MM-10T (96.7 %), Ponticoccus litoralis CL-GR66T (96.4 %) and Aquicoccus porphyridii L1 8-17T (96.1 %). The predominant fatty acids (>10 %) were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c; 72.1 %) and C16 : 0 (11.0 %). The polar lipids contain phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, one aminophosphlipid, one phospholipid and three unidentified lipids. The respiratory quinone was Q-10. The DNA G+C content was 63.0 mol% (data from the genome sequence). The estimated genome size was 5.12 Mb. The average nucleotide identity values between the DP3N28-2T genome and the genome of M. alba was 81.1 %, while the digital DNA-DNA hybridization value was 23.4 %. The phenotypic, genotypic and chemotaxonomic differences between DP3N28-2T and its phylogenetic relatives indicates that DP3N28-2T should be regarded as representing a novel species of the genus Mameliella, for which the name Mameliella sediminis sp. nov. is proposed. The type strain is DP3N28-2T (=MCCC 1K06218T=KCTC 82804T).