Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar).
Master's thesis in Environmental technology In order to safely dispose and use treated drill cuttings waste oil based mud, it is important to obtain knowledge about the contamination levels for possible adverse effects in freshwater organisms. Selected biomarkers were studied in Atlantic salmon...
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University of Stavanger, Norway
2014
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ftunivstavanger:oai:uis.brage.unit.no:11250/222719 2023-06-11T04:10:18+02:00 Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). Augustave, Lovasoa Cedrique 2014-07-14 application/pdf http://hdl.handle.net/11250/222719 eng eng University of Stavanger, Norway Masteroppgave/UIS-TN-IMN/2014; http://hdl.handle.net/11250/222719 CC0 1.0 Universal http://creativecommons.org/publicdomain/zero/1.0/ 50 drilling waste histopathology salmo salar biomarkers teknisk miljøvern VDP::Technology: 500::Environmental engineering: 610 Master thesis 2014 ftunivstavanger 2023-05-29T16:01:55Z Master's thesis in Environmental technology In order to safely dispose and use treated drill cuttings waste oil based mud, it is important to obtain knowledge about the contamination levels for possible adverse effects in freshwater organisms. Selected biomarkers were studied in Atlantic salmon (Salmo salar) exposed to treated and untreated drill cuttings waste (OBMs). The fish were exposed for 14 days in a continuous flow system to nominal concentrations of 0.1 and 1 ppm drill cuttings waste. Sampling was done 3 times during the exposure period but only samples at 14 days of exposure were object of histopathological analysis. Polycyclic aromatic hydrocarbons and heavy metal content in the freshwater were analysed at the end of the exposure period. Histopathological biomarkers of effect were studied then in gills and liver of fish by means of image analysis. Results showed that gills and liver were severely damaged with the high untreated group (1 ppm) to a lower extent with the high treated group and no considerable effects with the control. Dunnett’s test was performed (only for gills data) to test and highlight the significant difference between exposed groups in comparison to the control. The affected gills were mostly damaged by aneuryisms, epithelial lifting and necrosis probably due to constituents of the mineral oils or heavy metals. The lack of proofs of the liver data made statistical analysis impossible for the liver histopathology. Particularly noteworthy is the sensitive response of this high order biomarker of effect compared to those at lower organisation level in this study, and compared to similar responses in another study where salmon exposed to crude oil. Master Thesis Atlantic salmon Salmo salar University of Stavanger: UiS Brage |
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Open Polar |
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University of Stavanger: UiS Brage |
op_collection_id |
ftunivstavanger |
language |
English |
topic |
drilling waste histopathology salmo salar biomarkers teknisk miljøvern VDP::Technology: 500::Environmental engineering: 610 |
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drilling waste histopathology salmo salar biomarkers teknisk miljøvern VDP::Technology: 500::Environmental engineering: 610 Augustave, Lovasoa Cedrique Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). |
topic_facet |
drilling waste histopathology salmo salar biomarkers teknisk miljøvern VDP::Technology: 500::Environmental engineering: 610 |
description |
Master's thesis in Environmental technology In order to safely dispose and use treated drill cuttings waste oil based mud, it is important to obtain knowledge about the contamination levels for possible adverse effects in freshwater organisms. Selected biomarkers were studied in Atlantic salmon (Salmo salar) exposed to treated and untreated drill cuttings waste (OBMs). The fish were exposed for 14 days in a continuous flow system to nominal concentrations of 0.1 and 1 ppm drill cuttings waste. Sampling was done 3 times during the exposure period but only samples at 14 days of exposure were object of histopathological analysis. Polycyclic aromatic hydrocarbons and heavy metal content in the freshwater were analysed at the end of the exposure period. Histopathological biomarkers of effect were studied then in gills and liver of fish by means of image analysis. Results showed that gills and liver were severely damaged with the high untreated group (1 ppm) to a lower extent with the high treated group and no considerable effects with the control. Dunnett’s test was performed (only for gills data) to test and highlight the significant difference between exposed groups in comparison to the control. The affected gills were mostly damaged by aneuryisms, epithelial lifting and necrosis probably due to constituents of the mineral oils or heavy metals. The lack of proofs of the liver data made statistical analysis impossible for the liver histopathology. Particularly noteworthy is the sensitive response of this high order biomarker of effect compared to those at lower organisation level in this study, and compared to similar responses in another study where salmon exposed to crude oil. |
format |
Master Thesis |
author |
Augustave, Lovasoa Cedrique |
author_facet |
Augustave, Lovasoa Cedrique |
author_sort |
Augustave, Lovasoa Cedrique |
title |
Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). |
title_short |
Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). |
title_full |
Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). |
title_fullStr |
Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). |
title_full_unstemmed |
Study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in Atlantic salmon (Salmo salar). |
title_sort |
study of treated and untreated oil based drilling waste using a biomarker approach: gill and liver histopathology in atlantic salmon (salmo salar). |
publisher |
University of Stavanger, Norway |
publishDate |
2014 |
url |
http://hdl.handle.net/11250/222719 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
50 |
op_relation |
Masteroppgave/UIS-TN-IMN/2014; http://hdl.handle.net/11250/222719 |
op_rights |
CC0 1.0 Universal http://creativecommons.org/publicdomain/zero/1.0/ |
_version_ |
1768384621331873792 |