Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard
We assessed the structure of microbial communities in the subglacial drainage system of the Werenskioldbreen glacier, Svalbard, which consists of three independent channels. Dome-shaped naled ice bodies that had been forming and releasing subglacial water in the glacial forefield during accumulation...
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Online Access: | https://hdl.handle.net/20.500.12128/13319 https://doi.org/10.1016/j.scitotenv.2020.138025 |
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ftunivsilesia:oai:rebus.us.edu.pl:20.500.12128/13319 2023-05-15T16:22:16+02:00 Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard Sułowicz, Sławomir Bondarczuk, Kinga Ignatiuk, Dariusz Jania, Jacek Piotrowska-Seget, Zofia 2020 https://hdl.handle.net/20.500.12128/13319 https://doi.org/10.1016/j.scitotenv.2020.138025 en eng "Science of the Total Environment" Vol. 723 (2020), art. no 138025 0048-9697 http://hdl.handle.net/20.500.12128/13319 doi:10.1016/j.scitotenv.2020.138025 Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska http://creativecommons.org/licenses/by-nc-nd/3.0/pl/ CC-BY-NC-ND microbial communities subglacial microbiome naled-ice bodies NGS of 16S rRNA info:eu-repo/semantics/article 2020 ftunivsilesia https://doi.org/20.500.12128/13319 https://doi.org/10.1016/j.scitotenv.2020.138025 2022-12-31T20:13:27Z We assessed the structure of microbial communities in the subglacial drainage system of the Werenskioldbreen glacier, Svalbard, which consists of three independent channels. Dome-shaped naled ice bodies that had been forming and releasing subglacial water in the glacial forefield during accumulations season were used to study glacial microbiome. We tested the hypothesis that the properties of the water transported by these channels are site-dependent and influence bacterial diversity. We therefore established the phylogenetic structure of the subglacial microbial communities using next generation sequencing (NGS) of the 16S rRNA gene and performed bioinformatics analyses. A total of 1409 OTUs (operational taxonomic units) belonged to 40 phyla; mostly Proteobacteria, Gracilibacteria, Bacteroidetes, Actinobacteria and Parcubacteria were identified. Sites located on the edge of Werenskioldbreen forefield (Angell, Kvisla) were mainly dominated by Betaproteobacteria. In the central site (Dusan) domination of Epsilonproteobacteria class was observed. Gracilibacteria (GN02) and Gammaproteobacteria represented the dominant taxa only in the sample Kvisla 2. Principal Coordinate Analysis (PCoA) of beta diversity revealed that phylogenetic profiles grouped in three different clusters according to the sampling site. Moreover, higher similarity of bacterial communities from Angell and Kvisla compared to Dusan was confirmed by cluster analysis and Venn diagrams. The highest alpha index values was measured in Dusan. Richness and phylogenetic diversity indices were significantly (p < .05) and positively correlated with pH values of subglacial water and negatively with concentration of Cl−, Br−, and NO3− anions. These anions negatively impacted the values of richness indices but positively correlated with abundance of some microbial phyla. Our results indicated that subglacial water from naled ice bodies offer the possibility to study the glacial microbiome. In the studied subglacial water, the microbial community structure ... Article in Journal/Newspaper glacier Svalbard The Repository of the University of Silesia (RE-BUŚ) Svalbard Venn ENVELOPE(9.281,9.281,63.350,63.350) Werenskioldbreen ENVELOPE(15.336,15.336,77.077,77.077) Science of The Total Environment 723 138025 |
institution |
Open Polar |
collection |
The Repository of the University of Silesia (RE-BUŚ) |
op_collection_id |
ftunivsilesia |
language |
English |
topic |
microbial communities subglacial microbiome naled-ice bodies NGS of 16S rRNA |
spellingShingle |
microbial communities subglacial microbiome naled-ice bodies NGS of 16S rRNA Sułowicz, Sławomir Bondarczuk, Kinga Ignatiuk, Dariusz Jania, Jacek Piotrowska-Seget, Zofia Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard |
topic_facet |
microbial communities subglacial microbiome naled-ice bodies NGS of 16S rRNA |
description |
We assessed the structure of microbial communities in the subglacial drainage system of the Werenskioldbreen glacier, Svalbard, which consists of three independent channels. Dome-shaped naled ice bodies that had been forming and releasing subglacial water in the glacial forefield during accumulations season were used to study glacial microbiome. We tested the hypothesis that the properties of the water transported by these channels are site-dependent and influence bacterial diversity. We therefore established the phylogenetic structure of the subglacial microbial communities using next generation sequencing (NGS) of the 16S rRNA gene and performed bioinformatics analyses. A total of 1409 OTUs (operational taxonomic units) belonged to 40 phyla; mostly Proteobacteria, Gracilibacteria, Bacteroidetes, Actinobacteria and Parcubacteria were identified. Sites located on the edge of Werenskioldbreen forefield (Angell, Kvisla) were mainly dominated by Betaproteobacteria. In the central site (Dusan) domination of Epsilonproteobacteria class was observed. Gracilibacteria (GN02) and Gammaproteobacteria represented the dominant taxa only in the sample Kvisla 2. Principal Coordinate Analysis (PCoA) of beta diversity revealed that phylogenetic profiles grouped in three different clusters according to the sampling site. Moreover, higher similarity of bacterial communities from Angell and Kvisla compared to Dusan was confirmed by cluster analysis and Venn diagrams. The highest alpha index values was measured in Dusan. Richness and phylogenetic diversity indices were significantly (p < .05) and positively correlated with pH values of subglacial water and negatively with concentration of Cl−, Br−, and NO3− anions. These anions negatively impacted the values of richness indices but positively correlated with abundance of some microbial phyla. Our results indicated that subglacial water from naled ice bodies offer the possibility to study the glacial microbiome. In the studied subglacial water, the microbial community structure ... |
format |
Article in Journal/Newspaper |
author |
Sułowicz, Sławomir Bondarczuk, Kinga Ignatiuk, Dariusz Jania, Jacek Piotrowska-Seget, Zofia |
author_facet |
Sułowicz, Sławomir Bondarczuk, Kinga Ignatiuk, Dariusz Jania, Jacek Piotrowska-Seget, Zofia |
author_sort |
Sułowicz, Sławomir |
title |
Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard |
title_short |
Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard |
title_full |
Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard |
title_fullStr |
Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard |
title_full_unstemmed |
Microbial communities from subglacial water of naled ice bodies in the forefield of Werenskioldbreen, Svalbard |
title_sort |
microbial communities from subglacial water of naled ice bodies in the forefield of werenskioldbreen, svalbard |
publishDate |
2020 |
url |
https://hdl.handle.net/20.500.12128/13319 https://doi.org/10.1016/j.scitotenv.2020.138025 |
long_lat |
ENVELOPE(9.281,9.281,63.350,63.350) ENVELOPE(15.336,15.336,77.077,77.077) |
geographic |
Svalbard Venn Werenskioldbreen |
geographic_facet |
Svalbard Venn Werenskioldbreen |
genre |
glacier Svalbard |
genre_facet |
glacier Svalbard |
op_relation |
"Science of the Total Environment" Vol. 723 (2020), art. no 138025 0048-9697 http://hdl.handle.net/20.500.12128/13319 doi:10.1016/j.scitotenv.2020.138025 |
op_rights |
Uznanie autorstwa-Użycie niekomercyjne-Bez utworów zależnych 3.0 Polska http://creativecommons.org/licenses/by-nc-nd/3.0/pl/ |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/20.500.12128/13319 https://doi.org/10.1016/j.scitotenv.2020.138025 |
container_title |
Science of The Total Environment |
container_volume |
723 |
container_start_page |
138025 |
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1766010236678701056 |