The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla)
TNT (2,4,6-trinitrotoluene) was the most common nitro aromatic explosive available in WorldWar II ammunitions. The presence of ordnance dumped at sea might represent a great concern for marine species living close to dumping sites and the toxicological properties of the chemicals released into the m...
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Online Access: | http://hdl.handle.net/11365/49907 https://doi.org/10.1042/BST0341228 |
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ftunivsiena:oai:usiena-air.unisi.it:11365/49907 2024-04-14T08:00:57+00:00 The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) Della Torre, C. Corsi, I. Alcaro, L. Amato, E. Focardi, S. Della Torre, C. Corsi, I. Alcaro, L. Amato, E. Focardi, S. 2006 STAMPA http://hdl.handle.net/11365/49907 https://doi.org/10.1042/BST0341228 eng eng info:eu-repo/semantics/altIdentifier/pmid/17073791 info:eu-repo/semantics/altIdentifier/wos/WOS:000243013500056 volume:34 issue:Part 6 firstpage:1228 lastpage:1230 numberofpages:3 journal:BIOCHEMICAL SOCIETY TRANSACTIONS http://hdl.handle.net/11365/49907 doi:10.1042/BST0341228 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34248645986 2,4,6-trinitrotoluene (TNT) 7-ethoxyresorufin-O-de-ethylase (EROD) Cytochrome P450 1A (CYP1A) European eel [Anguilla anguilla (Linnaeus 1758)] NADPH cytochrome c reductase UDP-glucuronosyltransferase info:eu-repo/semantics/article 2006 ftunivsiena https://doi.org/10.1042/BST0341228 2024-03-21T16:12:31Z TNT (2,4,6-trinitrotoluene) was the most common nitro aromatic explosive available in WorldWar II ammunitions. The presence of ordnance dumped at sea might represent a great concern for marine species living close to dumping sites and the toxicological properties of the chemicals released into the marine environments need to be evaluated. The aim of the present study is to investigate the involvement of CYP (cytochrome P450) system in the metabolism of TNT in marine organisms by using the European eel [Anguilla anguilla (Linnaeus, 1758)] as model species. In vivo exposure to sublethal concentration of TNT (0.5, 1 and 2.5 mg/l) leads to a significant decrease in the phase I CYP1A catalytic activities such as EROD (7-ethoxyresorufin-O-de-ethylase) and MROD (7-methoxyresorufin-O-de-ethylase). On the opposite, a significant increase in NADPH cytochrome c reductase activity aswell as phase II UDP- glucuronosyltransferase activity is observed. An inhibition at enzyme level is hypothesized for both CYP1A enzymes, also confirmed by a similar decrease observed after in vitro exposure. An active role of NADPH cytochrome c reductase and phase II enzymes in the TNT metabolism may also be hypothesized. ©2006 Biochemical Society. Article in Journal/Newspaper Anguilla anguilla European eel Università degli Studi di Siena: USiena air Biochemical Society Transactions 34 6 1228 1230 |
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Università degli Studi di Siena: USiena air |
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ftunivsiena |
language |
English |
topic |
2,4,6-trinitrotoluene (TNT) 7-ethoxyresorufin-O-de-ethylase (EROD) Cytochrome P450 1A (CYP1A) European eel [Anguilla anguilla (Linnaeus 1758)] NADPH cytochrome c reductase UDP-glucuronosyltransferase |
spellingShingle |
2,4,6-trinitrotoluene (TNT) 7-ethoxyresorufin-O-de-ethylase (EROD) Cytochrome P450 1A (CYP1A) European eel [Anguilla anguilla (Linnaeus 1758)] NADPH cytochrome c reductase UDP-glucuronosyltransferase Della Torre, C. Corsi, I. Alcaro, L. Amato, E. Focardi, S. The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) |
topic_facet |
2,4,6-trinitrotoluene (TNT) 7-ethoxyresorufin-O-de-ethylase (EROD) Cytochrome P450 1A (CYP1A) European eel [Anguilla anguilla (Linnaeus 1758)] NADPH cytochrome c reductase UDP-glucuronosyltransferase |
description |
TNT (2,4,6-trinitrotoluene) was the most common nitro aromatic explosive available in WorldWar II ammunitions. The presence of ordnance dumped at sea might represent a great concern for marine species living close to dumping sites and the toxicological properties of the chemicals released into the marine environments need to be evaluated. The aim of the present study is to investigate the involvement of CYP (cytochrome P450) system in the metabolism of TNT in marine organisms by using the European eel [Anguilla anguilla (Linnaeus, 1758)] as model species. In vivo exposure to sublethal concentration of TNT (0.5, 1 and 2.5 mg/l) leads to a significant decrease in the phase I CYP1A catalytic activities such as EROD (7-ethoxyresorufin-O-de-ethylase) and MROD (7-methoxyresorufin-O-de-ethylase). On the opposite, a significant increase in NADPH cytochrome c reductase activity aswell as phase II UDP- glucuronosyltransferase activity is observed. An inhibition at enzyme level is hypothesized for both CYP1A enzymes, also confirmed by a similar decrease observed after in vitro exposure. An active role of NADPH cytochrome c reductase and phase II enzymes in the TNT metabolism may also be hypothesized. ©2006 Biochemical Society. |
author2 |
Della Torre, C. Corsi, I. Alcaro, L. Amato, E. Focardi, S. |
format |
Article in Journal/Newspaper |
author |
Della Torre, C. Corsi, I. Alcaro, L. Amato, E. Focardi, S. |
author_facet |
Della Torre, C. Corsi, I. Alcaro, L. Amato, E. Focardi, S. |
author_sort |
Della Torre, C. |
title |
The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) |
title_short |
The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) |
title_full |
The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) |
title_fullStr |
The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) |
title_full_unstemmed |
The involvement of cytochrome P450 system in the fate of 2,4,6-trinitrotoluene in European eel (Anguilla anguilla) |
title_sort |
involvement of cytochrome p450 system in the fate of 2,4,6-trinitrotoluene in european eel (anguilla anguilla) |
publishDate |
2006 |
url |
http://hdl.handle.net/11365/49907 https://doi.org/10.1042/BST0341228 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/17073791 info:eu-repo/semantics/altIdentifier/wos/WOS:000243013500056 volume:34 issue:Part 6 firstpage:1228 lastpage:1230 numberofpages:3 journal:BIOCHEMICAL SOCIETY TRANSACTIONS http://hdl.handle.net/11365/49907 doi:10.1042/BST0341228 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-34248645986 |
op_doi |
https://doi.org/10.1042/BST0341228 |
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Biochemical Society Transactions |
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34 |
container_issue |
6 |
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1228 |
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1230 |
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1796305591213228032 |