Induction of EROD activity in European eel (Anguilla anguilla) experimentally exposed to B(a)P and βNP

• Published in: Environment International
• Main Authors: BONACCI, S., CORSI, I., CHIEA, R., REGOLI, S., FOCARDI, S.
• Other Authors: Bonacci, S., Corsi, I., Chiea, R., Regoli, S., Focardi, S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2003
• Subjects: benzo[a]pyrene; beta-naphthoflavone; CYP 1A1; EROD; Anguilla anguilla
• Online Access: http://hdl.handle.net/11365/44709; https://doi.org/10.1016/S0160-4120(03)00005-9

The induction of liver ethoxyresorufin O-deethylase (EROD) activity was investigated in the European eel, Anguilla anguilla, collected from a Mediterranean brackish environment and experimentally exposed to benzo[a]pyrene (B[a]P) and h-naphthoflavone (BNF). Eels were injected intraperitoneally at increasing doses (0.1, 1, 10, and 50 mg/kg wet body weight) using corn oil as a carrier and sacrificed after 7 days. The main objectives of the present study are: (1) to assess of the sensitivity of EROD induction as a biomarker to polycyclic aromatic hydrocarbon (PAH) exposure; (2) to determine an EROD dose–response relationship of the contaminants used; and (3) to compare the efficiency of B[a]P and BNF as inducers of EROD activity. Results showed that both chemicals resulted in a dose-dependent EROD induction, but increases were not linear. EROD activity seemed to reach a plateau at the exposure of 10 mg/kg in both treatment groups; B[a]P was a more potent inducer than BNF was at the higher doses (10 and 50 mg/kg), while the opposite result was observed at the lower ones (0.1 and 1 mg/kg). The greatest induction occurred in eels treated with 10 mg/kg B[a]P, in which a 261-fold increase in EROD activity was observed. Results showed that EROD activity in A. anguilla is significantly induced by B[a]P and BNF exposure, responding to a wide range of concentrations of these contaminants. We infer that this tool may be suited as a diagnostic biomarker for biomonitoring PAHs pollution in Mediterranean brackish environments and further field research is suggested