Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758)
The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6 h and 24 h to 0.5, 1 and 2.5 mg/L nominal concentrations of TNT. E...
Published in: | Marine Environmental Research |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2008
|
Subjects: | |
Online Access: | http://hdl.handle.net/11365/4305 https://doi.org/10.1016/j.marenvres.2008.02.008 |
id |
ftunivsiena:oai:usiena-air.unisi.it:11365/4305 |
---|---|
record_format |
openpolar |
spelling |
ftunivsiena:oai:usiena-air.unisi.it:11365/4305 2024-02-11T09:55:29+01:00 Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) DELLA TORRE, C. CORSI, I. ARUKWE, A. ALCARO, L. AMATO, E. FOCARDI, S. DELLA TORRE, C. Corsi, I. Arukwe, A. Alcaro, L. Amato, E. Focardi, S. 2008 STAMPA http://hdl.handle.net/11365/4305 https://doi.org/10.1016/j.marenvres.2008.02.008 eng eng info:eu-repo/semantics/altIdentifier/pmid/18378298 info:eu-repo/semantics/altIdentifier/wos/WOS:000257575400005 volume:66 issue:1 firstpage:9 lastpage:11 numberofpages:3 journal:MARINE ENVIRONMENTAL RESEARCH http://hdl.handle.net/11365/4305 doi:10.1016/j.marenvres.2008.02.008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-44749083437 info:eu-repo/semantics/closedAccess 2,4,6-Trinitrotoluene CYP1A European eel GST UDPGT info:eu-repo/semantics/article 2008 ftunivsiena https://doi.org/10.1016/j.marenvres.2008.02.008 2024-01-23T23:17:18Z The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6 h and 24 h to 0.5, 1 and 2.5 mg/L nominal concentrations of TNT. Expression of CYPIA, glutathione-S-transferase (pi-class; CST) and uridine-diphosphate glucuronosyltransferase (]family) (UDPGT) genes was investigated by RT-PCR, and 7-ethoxy- and 7-methoxyresorufln-0-deall :ylases (EROD, MROD), NADPH cyt c reductase (NADPH red), UDPGT and CST enzyme activities were measured by biochemical assays. An in vitro study was also performed, measuring only EROD activity. TNT exposure produced no modulation of CYPlA transcript expression while a significant inhibition of EROD enzyme activity was observed and confirmed in vitro. UDPGT transcript increased closedependently only at 6 h while the UDPGT activity tended to increase dose-dependently at 24 h. CST gene expression increased after 24 h and significant increases of CST activity were observed both at 6 and 24 h only at the highest TNT concentration. An increase of NADPH red activity was observed at 24 h. Our results seem to indicate an inhibitory effect of TNT on CYPI A-dependent catalytic activities and a possible involvement of phase 11 enzymes as well as NADPH red in TNT metabolism in eels. (C) 2008 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Anguilla anguilla European eel Università degli Studi di Siena: USiena air Marine Environmental Research 66 1 9 11 |
institution |
Open Polar |
collection |
Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
2,4,6-Trinitrotoluene CYP1A European eel GST UDPGT |
spellingShingle |
2,4,6-Trinitrotoluene CYP1A European eel GST UDPGT DELLA TORRE, C. CORSI, I. ARUKWE, A. ALCARO, L. AMATO, E. FOCARDI, S. Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
topic_facet |
2,4,6-Trinitrotoluene CYP1A European eel GST UDPGT |
description |
The aim of this study was to investigate effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver drug metabolizing genes and enzymes in the European eel Anguilla anguilla as a model fish species. Eels were exposed in vivo for 6 h and 24 h to 0.5, 1 and 2.5 mg/L nominal concentrations of TNT. Expression of CYPIA, glutathione-S-transferase (pi-class; CST) and uridine-diphosphate glucuronosyltransferase (]family) (UDPGT) genes was investigated by RT-PCR, and 7-ethoxy- and 7-methoxyresorufln-0-deall :ylases (EROD, MROD), NADPH cyt c reductase (NADPH red), UDPGT and CST enzyme activities were measured by biochemical assays. An in vitro study was also performed, measuring only EROD activity. TNT exposure produced no modulation of CYPlA transcript expression while a significant inhibition of EROD enzyme activity was observed and confirmed in vitro. UDPGT transcript increased closedependently only at 6 h while the UDPGT activity tended to increase dose-dependently at 24 h. CST gene expression increased after 24 h and significant increases of CST activity were observed both at 6 and 24 h only at the highest TNT concentration. An increase of NADPH red activity was observed at 24 h. Our results seem to indicate an inhibitory effect of TNT on CYPI A-dependent catalytic activities and a possible involvement of phase 11 enzymes as well as NADPH red in TNT metabolism in eels. (C) 2008 Elsevier Ltd. All rights reserved. |
author2 |
DELLA TORRE, C. Corsi, I. Arukwe, A. Alcaro, L. Amato, E. Focardi, S. |
format |
Article in Journal/Newspaper |
author |
DELLA TORRE, C. CORSI, I. ARUKWE, A. ALCARO, L. AMATO, E. FOCARDI, S. |
author_facet |
DELLA TORRE, C. CORSI, I. ARUKWE, A. ALCARO, L. AMATO, E. FOCARDI, S. |
author_sort |
DELLA TORRE, C. |
title |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_short |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_full |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_fullStr |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_full_unstemmed |
Effects of 2,4,6-trinitrotoluene (TNT) on phase I and phase II biotransformation enzymes in European eel Anguilla anguilla (Linnaeus, 1758) |
title_sort |
effects of 2,4,6-trinitrotoluene (tnt) on phase i and phase ii biotransformation enzymes in european eel anguilla anguilla (linnaeus, 1758) |
publishDate |
2008 |
url |
http://hdl.handle.net/11365/4305 https://doi.org/10.1016/j.marenvres.2008.02.008 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/18378298 info:eu-repo/semantics/altIdentifier/wos/WOS:000257575400005 volume:66 issue:1 firstpage:9 lastpage:11 numberofpages:3 journal:MARINE ENVIRONMENTAL RESEARCH http://hdl.handle.net/11365/4305 doi:10.1016/j.marenvres.2008.02.008 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-44749083437 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.marenvres.2008.02.008 |
container_title |
Marine Environmental Research |
container_volume |
66 |
container_issue |
1 |
container_start_page |
9 |
op_container_end_page |
11 |
_version_ |
1790596638062936064 |