Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd
Several ABC transporters have been characterized from many aquatic organisms, but no information is yet available for Antarctic fish. The aim of this work was to identify the expression of genes for ABC proteins in Trematomus bernacchii, a bioindicator species of the Southern Ocean. Partial cDNA seq...
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ftunivsiena:oai:usiena-air.unisi.it:11365/3454 2024-04-14T08:01:26+00:00 Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd ZUCCHI, S. CORSI, I. LUCKENBACH, T. BARD, S. M. REGOLI, F. FOCARDI, S. Zucchi, S. Corsi, I. Luckenbach, T. Bard, S. M. Regoli, F. Focardi, S. 2010 STAMPA http://hdl.handle.net/11365/3454 https://doi.org/10.1016/j.envpol.2010.04.012 eng eng info:eu-repo/semantics/altIdentifier/pmid/20627496 info:eu-repo/semantics/altIdentifier/wos/WOS:000280571500032 volume:158 issue:8 firstpage:2746 lastpage:2756 numberofpages:11 journal:ENVIRONMENTAL POLLUTION http://hdl.handle.net/11365/3454 doi:10.1016/j.envpol.2010.04.012 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-77958189954 info:eu-repo/semantics/closedAccess ABC transporter Antarctica Cadmium Liver Trematomus bernacchii info:eu-repo/semantics/article 2010 ftunivsiena https://doi.org/10.1016/j.envpol.2010.04.012 2024-03-21T15:54:55Z Several ABC transporters have been characterized from many aquatic organisms, but no information is yet available for Antarctic fish. The aim of this work was to identify the expression of genes for ABC proteins in Trematomus bernacchii, a bioindicator species of the Southern Ocean. Partial cDNA sequences of ABCB1, ABCC1, ABCC2, ABCC4 and ABCC9 were cloned from liver. Using RACE technology, 3.5 and 2.2 kb contigs were obtained for ABCB1 and ABCC2. Considering the elevated natural bioavailability of cadmium at Terra Nova Bay, responsiveness of ABCB1 and ABCC2 to this element was investigated under laboratory conditions. ABCB1 and ABCC2 mRNA levels were approximately four-fold higher in Cd-exposed fish compared to the controls. Induction of ABCB1 protein was also found by western blot. This study provides the first identification of five ABC genes in the liver of an Antarctic key species, some of which may be involved in cellular detoxification. © 2010 Elsevier Ltd. All rights reserved. Article in Journal/Newspaper Antarc* Antarctic Antarctica Southern Ocean Università degli Studi di Siena: USiena air Antarctic Southern Ocean Terra Nova Bay The Antarctic Environmental Pollution 158 8 2746 2756 |
institution |
Open Polar |
collection |
Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
ABC transporter Antarctica Cadmium Liver Trematomus bernacchii |
spellingShingle |
ABC transporter Antarctica Cadmium Liver Trematomus bernacchii ZUCCHI, S. CORSI, I. LUCKENBACH, T. BARD, S. M. REGOLI, F. FOCARDI, S. Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd |
topic_facet |
ABC transporter Antarctica Cadmium Liver Trematomus bernacchii |
description |
Several ABC transporters have been characterized from many aquatic organisms, but no information is yet available for Antarctic fish. The aim of this work was to identify the expression of genes for ABC proteins in Trematomus bernacchii, a bioindicator species of the Southern Ocean. Partial cDNA sequences of ABCB1, ABCC1, ABCC2, ABCC4 and ABCC9 were cloned from liver. Using RACE technology, 3.5 and 2.2 kb contigs were obtained for ABCB1 and ABCC2. Considering the elevated natural bioavailability of cadmium at Terra Nova Bay, responsiveness of ABCB1 and ABCC2 to this element was investigated under laboratory conditions. ABCB1 and ABCC2 mRNA levels were approximately four-fold higher in Cd-exposed fish compared to the controls. Induction of ABCB1 protein was also found by western blot. This study provides the first identification of five ABC genes in the liver of an Antarctic key species, some of which may be involved in cellular detoxification. © 2010 Elsevier Ltd. All rights reserved. |
author2 |
Zucchi, S. Corsi, I. Luckenbach, T. Bard, S. M. Regoli, F. Focardi, S. |
format |
Article in Journal/Newspaper |
author |
ZUCCHI, S. CORSI, I. LUCKENBACH, T. BARD, S. M. REGOLI, F. FOCARDI, S. |
author_facet |
ZUCCHI, S. CORSI, I. LUCKENBACH, T. BARD, S. M. REGOLI, F. FOCARDI, S. |
author_sort |
ZUCCHI, S. |
title |
Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd |
title_short |
Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd |
title_full |
Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd |
title_fullStr |
Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd |
title_full_unstemmed |
Identification of five partial ABC-transporter genes in the liver of the Antarctic fish Trematomus bernacchii: sensitivity of ABCB1 and ABCC2 to Cd |
title_sort |
identification of five partial abc-transporter genes in the liver of the antarctic fish trematomus bernacchii: sensitivity of abcb1 and abcc2 to cd |
publishDate |
2010 |
url |
http://hdl.handle.net/11365/3454 https://doi.org/10.1016/j.envpol.2010.04.012 |
geographic |
Antarctic Southern Ocean Terra Nova Bay The Antarctic |
geographic_facet |
Antarctic Southern Ocean Terra Nova Bay The Antarctic |
genre |
Antarc* Antarctic Antarctica Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Southern Ocean |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/20627496 info:eu-repo/semantics/altIdentifier/wos/WOS:000280571500032 volume:158 issue:8 firstpage:2746 lastpage:2756 numberofpages:11 journal:ENVIRONMENTAL POLLUTION http://hdl.handle.net/11365/3454 doi:10.1016/j.envpol.2010.04.012 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-77958189954 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.envpol.2010.04.012 |
container_title |
Environmental Pollution |
container_volume |
158 |
container_issue |
8 |
container_start_page |
2746 |
op_container_end_page |
2756 |
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1796308907956633600 |