Alicyclobacillus pohliae sp. nov., a novel thermophilic endospore-forming bacterium isolated from geothermal soil of the north-west slope of Mount Melbourne (Antarctica)

• Published in: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
• Main Authors: IMPERIO T., VTI C., MARRI, LAURA
• Other Authors: Imperio, T., Vti, C., Marri, Laura
• Format: Article in Journal/Newspaper
• Language: English
• Published: SOC GENERAL MICROBIOLOGY, MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING, ENGLAND, BERKS, RG7 1AG 2008
• Subjects: Antarc*; Antarctica
• Online Access: http://hdl.handle.net/11365/21499; https://doi.org/10.1099/ijs.0.65092-0

Gram-positive, rod-shaped, endospore-forming, thermophilic bacteria were isolated from a geothermal soil collected on the north-west slope of Mount Melbourne in Antarctica. They grew aerobically at 42–60°C (optimum 55°C) and at pH 4.5–7.5 (optimum pH 5.5). Phylogenetic analysis of 16S rRNA gene sequences showed that these isolates were related most closely to the type strain of Alicyclobacillus pomorum (91% similarity). Growth occurred in the presence of ferrous iron at micromolar concentrations and acid was produced from various sugars. Iso-branched fatty acids C15 : 0 (45.56 %) and C17 : 0 (35.81 %) were the most abundant cellular fatty acids. The DNA G+C content was 55.1 mol%. On the basis of phenotypic and phylogenetic characteristics, it is concluded that these strains represent a novel species of the genus Alicyclobacillus, for which the name Alicyclobacillus pohliae sp. nov. is proposed. The type strain is MP4T (=CIP 109385T =NCIMB 14276T).