Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure
Populations of killer whale (Orcinus orca) contain some of the most polluted animals on Earth. Yet, the knowl-edge on effects of chemical pollutants is limited in this species. Cell cultures and in vitro exposure experiments are pertinent tools to study effects of pollutants in free-ranging marine m...
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Online Access: | https://hdl.handle.net/11365/1233154 https://doi.org/10.1016/j.envint.2023.107915 https://www.sciencedirect.com/science/article/pii/S0160412023001885?via=ihub |
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ftunivsiena:oai:usiena-air.unisi.it:11365/1233154 2024-04-14T08:14:22+00:00 Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure Bjørneset, J. Blévin, P. Bjørnstad, P. M. Dalmo, R. A. Goksøyr, A. Harju, M. Limonta, G. Panti, C. Rikardsen, A. H. Sundaram, A. Y. M. Yadetie, F. Routti, H. Bjørneset, J. Blévin, P. Bjørnstad, P. M. Dalmo, R. A. Goksøyr, A. Harju, M. Limonta, G. Panti, C. Rikardsen, A. H. Sundaram, A. Y. M. Yadetie, F. Routti, H. 2023 ELETTRONICO https://hdl.handle.net/11365/1233154 https://doi.org/10.1016/j.envint.2023.107915 https://www.sciencedirect.com/science/article/pii/S0160412023001885?via=ihub eng eng info:eu-repo/semantics/altIdentifier/pmid/37031518 info:eu-repo/semantics/altIdentifier/wos/WOS:000982146200001 volume:174 journal:ENVIRONMENT INTERNATIONAL https://hdl.handle.net/11365/1233154 doi:10.1016/j.envint.2023.107915 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85151753551 https://www.sciencedirect.com/science/article/pii/S0160412023001885?via=ihub info:eu-repo/semantics/openAccess Cell culture In vitro Marine mammal Mixture Persistent organic pollutant RNA-seq info:eu-repo/semantics/article 2023 ftunivsiena https://doi.org/10.1016/j.envint.2023.107915 2024-03-21T15:51:40Z Populations of killer whale (Orcinus orca) contain some of the most polluted animals on Earth. Yet, the knowl-edge on effects of chemical pollutants is limited in this species. Cell cultures and in vitro exposure experiments are pertinent tools to study effects of pollutants in free-ranging marine mammals. To investigate transcriptional responses to pollutants in killer whale cells, we collected skin biopsies of killer whales from the Northern Norwegian fjords and successfully established primary fibroblast cell cultures from the dermis of 4 out of 5 of them. Cells from the individual with the highest cell yield were exposed to three different concentrations of a mixture of persistent organic pollutants (POPs) that reflects the composition of the 10 most abundant POPs found in Norwegian killer whales (p,p'-DDE, trans-nonachlor, PCB52, 99, 101, 118, 138, 153, 180, 187). Transcrip-tional responses of 13 selected target genes were studied using digital droplet PCR, and whole transcriptome responses were investigated utilizing RNA sequencing. Among the target genes analysed, CYP1A1 was signifi-cantly downregulated in the cells exposed to medium (11.6 mu M) and high (116 mu M) concentrations of the pollutant mixture, while seven genes involved in endocrine functions showed a non-significant tendency to be upregulated at the highest exposure concentration. Bioinformatic analyses of RNA-seq data indicated that 13 and 43 genes were differentially expressed in the cells exposed to low and high concentrations of the mixture, respectively, in comparison to solvent control. Subsequent pathway and functional analyses of the differentially expressed genes indicated that the enriched pathways were mainly related to lipid metabolism, myogenesis and glucocorticoid receptor regulation. The current study results support previous correlative studies and provide cause-effect relationships, which is highly relevant for chemical and environmental management. Article in Journal/Newspaper Killer Whale Orca Orcinus orca Killer whale Università degli Studi di Siena: USiena air Environment International 174 107915 |
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Open Polar |
collection |
Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
Cell culture In vitro Marine mammal Mixture Persistent organic pollutant RNA-seq |
spellingShingle |
Cell culture In vitro Marine mammal Mixture Persistent organic pollutant RNA-seq Bjørneset, J. Blévin, P. Bjørnstad, P. M. Dalmo, R. A. Goksøyr, A. Harju, M. Limonta, G. Panti, C. Rikardsen, A. H. Sundaram, A. Y. M. Yadetie, F. Routti, H. Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
topic_facet |
Cell culture In vitro Marine mammal Mixture Persistent organic pollutant RNA-seq |
description |
Populations of killer whale (Orcinus orca) contain some of the most polluted animals on Earth. Yet, the knowl-edge on effects of chemical pollutants is limited in this species. Cell cultures and in vitro exposure experiments are pertinent tools to study effects of pollutants in free-ranging marine mammals. To investigate transcriptional responses to pollutants in killer whale cells, we collected skin biopsies of killer whales from the Northern Norwegian fjords and successfully established primary fibroblast cell cultures from the dermis of 4 out of 5 of them. Cells from the individual with the highest cell yield were exposed to three different concentrations of a mixture of persistent organic pollutants (POPs) that reflects the composition of the 10 most abundant POPs found in Norwegian killer whales (p,p'-DDE, trans-nonachlor, PCB52, 99, 101, 118, 138, 153, 180, 187). Transcrip-tional responses of 13 selected target genes were studied using digital droplet PCR, and whole transcriptome responses were investigated utilizing RNA sequencing. Among the target genes analysed, CYP1A1 was signifi-cantly downregulated in the cells exposed to medium (11.6 mu M) and high (116 mu M) concentrations of the pollutant mixture, while seven genes involved in endocrine functions showed a non-significant tendency to be upregulated at the highest exposure concentration. Bioinformatic analyses of RNA-seq data indicated that 13 and 43 genes were differentially expressed in the cells exposed to low and high concentrations of the mixture, respectively, in comparison to solvent control. Subsequent pathway and functional analyses of the differentially expressed genes indicated that the enriched pathways were mainly related to lipid metabolism, myogenesis and glucocorticoid receptor regulation. The current study results support previous correlative studies and provide cause-effect relationships, which is highly relevant for chemical and environmental management. |
author2 |
Bjørneset, J. Blévin, P. Bjørnstad, P. M. Dalmo, R. A. Goksøyr, A. Harju, M. Limonta, G. Panti, C. Rikardsen, A. H. Sundaram, A. Y. M. Yadetie, F. Routti, H. |
format |
Article in Journal/Newspaper |
author |
Bjørneset, J. Blévin, P. Bjørnstad, P. M. Dalmo, R. A. Goksøyr, A. Harju, M. Limonta, G. Panti, C. Rikardsen, A. H. Sundaram, A. Y. M. Yadetie, F. Routti, H. |
author_facet |
Bjørneset, J. Blévin, P. Bjørnstad, P. M. Dalmo, R. A. Goksøyr, A. Harju, M. Limonta, G. Panti, C. Rikardsen, A. H. Sundaram, A. Y. M. Yadetie, F. Routti, H. |
author_sort |
Bjørneset, J. |
title |
Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
title_short |
Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
title_full |
Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
title_fullStr |
Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
title_full_unstemmed |
Establishment of killer whale (Orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
title_sort |
establishment of killer whale (orcinus orca) primary fibroblast cell cultures and their transcriptomic responses to pollutant exposure |
publishDate |
2023 |
url |
https://hdl.handle.net/11365/1233154 https://doi.org/10.1016/j.envint.2023.107915 https://www.sciencedirect.com/science/article/pii/S0160412023001885?via=ihub |
genre |
Killer Whale Orca Orcinus orca Killer whale |
genre_facet |
Killer Whale Orca Orcinus orca Killer whale |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/37031518 info:eu-repo/semantics/altIdentifier/wos/WOS:000982146200001 volume:174 journal:ENVIRONMENT INTERNATIONAL https://hdl.handle.net/11365/1233154 doi:10.1016/j.envint.2023.107915 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85151753551 https://www.sciencedirect.com/science/article/pii/S0160412023001885?via=ihub |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.envint.2023.107915 |
container_title |
Environment International |
container_volume |
174 |
container_start_page |
107915 |
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1796312550217875456 |