Behavioural, physiological and molecular responses of the Antarctic fairy shrimp Branchinecta gaini (Daday, 1910) to polystyrene nanoplastics

• Published in: NanoImpact
• Main Authors: Bergami, E., Krupinski Emerenciano, A., Palmeira Pinto, L., Reina Joviano, W., Font, A., Almeida de Godoy, T., Silva, J. M. C., González-Aravena, M., Corsi, I.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2022
• Subjects: Antarctica; Ecotoxicity; Freshwater microcrustacean; Plastic pollution; Polystyrene nanoparticle; South Shetland Islands; Antarc*; Antarctic; Antarctic Peninsula
• Online Access: https://hdl.handle.net/11365/1224296; https://doi.org/10.1016/j.impact.2022.100437; https://www.sciencedirect.com/science/article/pii/S2452074822000593

Plastic pollution represents an emerging environmental issue in terrestrial Antarctica, especially in the Antarctic Peninsula and Maritime Antarctica, which have been recently recognized as hot spots for plastic litter. In these regions, freshwater (FW) environments such as lakes host isolated ecosystems and species that can be severely affected by increasing environmental and anthropogenic stressors, which include plastics that are still overlooked. In this study, we investigated for the first time the impact of nanoplastics on adults of the fairy shrimp Branchinecta gaini (Order Anostraca) populating Antarctic FW ecosystems, using surface charged polystyrene nanoparticles (PS NPs) as a proxy. Short-term acute toxicity (48h) was investigated by exposing adults to carboxyl (-COOH, 60nm) and amino-modified (-NH2, 50nm) PS NPs at 1 and 5μgmL-1. Biodisposition of PS NPs and lethal and sub-lethal effects (i.e., swimming, moulting, histology, gene expression) were assessed. Behaviour of PS NPs in Antarctic FW media was monitored through 48h of exposure showing that both PS NPs kept their nanoscale size in the Antarctic FW media. Survival of fairy shrimp adults over short-term exposure was not affected, on the other hand an increase in moulting rate and alterations in the gut epithelium were observed upon exposure to both PS NPs. Significant alterations at the behavioural (ventilation rate) and molecular (up-regulation of Hsp70mit, Hsp83, Sod, P450) levels were related to PS NP surface charge and associated with PS-NH2 exposure only. Nanoplastics could represent a threat for Antarctic FW biodiversity and the Antarctic fairy shrimp could be a valuable model for assessing their impact on such remote and pristine aquatic ecosystems.