Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples
Sex identification is crucial for behavioral, ecological and conservation studies. In monomorphic bird species, traditional methods for sex assessment require potentially invasive sampling and manipulation of individuals, such as through cloacal examination. Thus, molecular methods involving non-des...
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Online Access: | https://hdl.handle.net/11365/1136100 https://doi.org/10.1675/063.043.0209 https://bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short |
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ftunivsiena:oai:usiena-air.unisi.it:11365/1136100 2024-04-14T08:18:38+00:00 Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples Mori, Emiliano Brunetti, Claudia Carapelli, Antonio Olmastroni, Silvia Mori, Emiliano Brunetti, Claudia Carapelli, Antonio Olmastroni, Silvia 2020 STAMPA https://hdl.handle.net/11365/1136100 https://doi.org/10.1675/063.043.0209 https://bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short eng eng info:eu-repo/semantics/altIdentifier/wos/WOS:000634574400009 volume:43 issue:2 firstpage:211 lastpage:216 numberofpages:6 journal:WATERBIRDS https://hdl.handle.net/11365/1136100 doi:10.1675/063.043.0209 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103236349 https://bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short info:eu-repo/semantics/closedAccess CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing info:eu-repo/semantics/article 2020 ftunivsiena https://doi.org/10.1675/063.043.0209 2024-03-21T16:09:56Z Sex identification is crucial for behavioral, ecological and conservation studies. In monomorphic bird species, traditional methods for sex assessment require potentially invasive sampling and manipulation of individuals, such as through cloacal examination. Thus, molecular methods involving non-destructive sampling are needed to reduce the stress of animals under study. In monomorphic Adélie penguins (Pygoscelis adeliae), the use of a penguin-specific primer pair (PL/PR) to identify sex has been evaluated through DNA extracted from nondestructive feather samples. With penguin-specific primers for the CHD1 gene found on the sex chromosomes, we efficiently identified the sex of all sampled individuals (n = 92, belonging to 46 complete nesting pairs in study colonies; n = 26 belonging to surveyed individuals in a control colony). DNA extraction and PCR protocols to improve amplification success are reported. Our results were also confirmed through Sanger sequencing used to determine the previously unpublished sequences of the CHD1 alleles in Adélie penguins, which were subsequently deposited in GenBank and used to compare to similar species. In phylogenetic reconstructions, CHD1Z and CHD1W sequences of the Adélie Penguin grouped together with other Pygoscelis and were the sister group of Spheniscus and Eudyptes genera. Article in Journal/Newspaper Pygoscelis adeliae Università degli Studi di Siena: USiena air Waterbirds 43 2 |
institution |
Open Polar |
collection |
Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing |
spellingShingle |
CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing Mori, Emiliano Brunetti, Claudia Carapelli, Antonio Olmastroni, Silvia Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
topic_facet |
CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing |
description |
Sex identification is crucial for behavioral, ecological and conservation studies. In monomorphic bird species, traditional methods for sex assessment require potentially invasive sampling and manipulation of individuals, such as through cloacal examination. Thus, molecular methods involving non-destructive sampling are needed to reduce the stress of animals under study. In monomorphic Adélie penguins (Pygoscelis adeliae), the use of a penguin-specific primer pair (PL/PR) to identify sex has been evaluated through DNA extracted from nondestructive feather samples. With penguin-specific primers for the CHD1 gene found on the sex chromosomes, we efficiently identified the sex of all sampled individuals (n = 92, belonging to 46 complete nesting pairs in study colonies; n = 26 belonging to surveyed individuals in a control colony). DNA extraction and PCR protocols to improve amplification success are reported. Our results were also confirmed through Sanger sequencing used to determine the previously unpublished sequences of the CHD1 alleles in Adélie penguins, which were subsequently deposited in GenBank and used to compare to similar species. In phylogenetic reconstructions, CHD1Z and CHD1W sequences of the Adélie Penguin grouped together with other Pygoscelis and were the sister group of Spheniscus and Eudyptes genera. |
author2 |
Mori, Emiliano Brunetti, Claudia Carapelli, Antonio Olmastroni, Silvia |
format |
Article in Journal/Newspaper |
author |
Mori, Emiliano Brunetti, Claudia Carapelli, Antonio Olmastroni, Silvia |
author_facet |
Mori, Emiliano Brunetti, Claudia Carapelli, Antonio Olmastroni, Silvia |
author_sort |
Mori, Emiliano |
title |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_short |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_full |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_fullStr |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_full_unstemmed |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_sort |
reliability of molecular sex identification in the adélie penguin (pygoscelis adeliae) from dna-poor samples |
publishDate |
2020 |
url |
https://hdl.handle.net/11365/1136100 https://doi.org/10.1675/063.043.0209 https://bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short |
genre |
Pygoscelis adeliae |
genre_facet |
Pygoscelis adeliae |
op_relation |
info:eu-repo/semantics/altIdentifier/wos/WOS:000634574400009 volume:43 issue:2 firstpage:211 lastpage:216 numberofpages:6 journal:WATERBIRDS https://hdl.handle.net/11365/1136100 doi:10.1675/063.043.0209 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85103236349 https://bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1675/063.043.0209 |
container_title |
Waterbirds |
container_volume |
43 |
container_issue |
2 |
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1796318164444774400 |