Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples
4 none Sex identification is crucial for behavioral, ecological and conservation studies. In monomorphic bird species, traditional methods for sex assessment require potentially invasive sampling and manipulation of individuals, such as through cloacal examination. Thus, molecular methods involving...
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2021
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Online Access: | http://hdl.handle.net/11365/1136100 https://complete.bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short https://doi.org/10.1675/063.043.0209 |
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ftunivsiena:oai:usiena-air.unisi.it:11365/1136100.2 2023-05-15T18:03:49+02:00 Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples Dipartimento di Medicina Molecolare e dello Sviluppo Dipartimento di Scienze Fisiche, della Terra e dell'Ambiente Dipartimento di Scienze della Vita 575 576 579 Non assegn AREA MIN. 05 - Scienze biologiche 2021-03-26T11:44:00Z http://hdl.handle.net/11365/1136100 https://complete.bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short https://doi.org/10.1675/063.043.0209 eng eng 2 ELETTRONICO 43 crossref 2-s2.0-85103236349 http://hdl.handle.net/11365/1136100 https://complete.bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short doi:10.1675/063.043.0209 Mori, E., Brunetti, C., Carapelli, A., & Olmastroni, S. (2020). Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples. WATERBIRDS, 43(2) [10.1675/063.043.0209]. CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing internazionale Esperti anonimi Si 262 1 Contributo su Rivista::1.1 Articolo in rivista info:eu-repo/semantics/article 2021 ftunivsiena https://doi.org/10.1675/063.043.0209 2022-08-16T22:27:42Z 4 none Sex identification is crucial for behavioral, ecological and conservation studies. In monomorphic bird species, traditional methods for sex assessment require potentially invasive sampling and manipulation of individuals, such as through cloacal examination. Thus, molecular methods involving non-destructive sampling are needed to reduce the stress of animals under study. In monomorphic Adélie penguins (Pygoscelis adeliae), the use of a penguin-specific primer pair (PL/PR) to identify sex has been evaluated through DNA extracted from nondestructive feather samples. With penguin-specific primers for the CHD1 gene found on the sex chromosomes, we efficiently identified the sex of all sampled individuals (n = 92, belonging to 46 complete nesting pairs in study colonies; n = 26 belonging to surveyed individuals in a control colony). DNA extraction and PCR protocols to improve amplification success are reported. Our results were also confirmed through Sanger sequencing used to determine the previously unpublished sequences of the CHD1 alleles in Adélie penguins, which were subsequently deposited in GenBank and used to compare to similar species. In phylogenetic reconstructions, CHD1Z and CHD1W sequences of the Adélie Penguin grouped together with other Pygoscelis and were the sister group of Spheniscus and Eudyptes genera. mixed Mori, Emiliano; Brunetti, Claudia; Carapelli, Antonio; Olmastroni, Silvia Mori, Emiliano; Brunetti, Claudia; Carapelli, Antonio; Olmastroni, Silvia Article in Journal/Newspaper Pygoscelis adeliae Università degli Studi di Siena: USiena air Silvia ENVELOPE(-57.900,-57.900,-63.300,-63.300) Waterbirds 43 2 |
institution |
Open Polar |
collection |
Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing |
spellingShingle |
CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
topic_facet |
CHD1 gene feathers molecular sex identification penguin phylogenetics Pygoscelis adeliae primers sexing |
description |
4 none Sex identification is crucial for behavioral, ecological and conservation studies. In monomorphic bird species, traditional methods for sex assessment require potentially invasive sampling and manipulation of individuals, such as through cloacal examination. Thus, molecular methods involving non-destructive sampling are needed to reduce the stress of animals under study. In monomorphic Adélie penguins (Pygoscelis adeliae), the use of a penguin-specific primer pair (PL/PR) to identify sex has been evaluated through DNA extracted from nondestructive feather samples. With penguin-specific primers for the CHD1 gene found on the sex chromosomes, we efficiently identified the sex of all sampled individuals (n = 92, belonging to 46 complete nesting pairs in study colonies; n = 26 belonging to surveyed individuals in a control colony). DNA extraction and PCR protocols to improve amplification success are reported. Our results were also confirmed through Sanger sequencing used to determine the previously unpublished sequences of the CHD1 alleles in Adélie penguins, which were subsequently deposited in GenBank and used to compare to similar species. In phylogenetic reconstructions, CHD1Z and CHD1W sequences of the Adélie Penguin grouped together with other Pygoscelis and were the sister group of Spheniscus and Eudyptes genera. mixed Mori, Emiliano; Brunetti, Claudia; Carapelli, Antonio; Olmastroni, Silvia Mori, Emiliano; Brunetti, Claudia; Carapelli, Antonio; Olmastroni, Silvia |
author2 |
Dipartimento di Medicina Molecolare e dello Sviluppo Dipartimento di Scienze Fisiche, della Terra e dell'Ambiente Dipartimento di Scienze della Vita 575 576 579 Non assegn AREA MIN. 05 - Scienze biologiche |
format |
Article in Journal/Newspaper |
title |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_short |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_full |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_fullStr |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_full_unstemmed |
Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples |
title_sort |
reliability of molecular sex identification in the adélie penguin (pygoscelis adeliae) from dna-poor samples |
publishDate |
2021 |
url |
http://hdl.handle.net/11365/1136100 https://complete.bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short https://doi.org/10.1675/063.043.0209 |
long_lat |
ENVELOPE(-57.900,-57.900,-63.300,-63.300) |
geographic |
Silvia |
geographic_facet |
Silvia |
genre |
Pygoscelis adeliae |
genre_facet |
Pygoscelis adeliae |
op_relation |
2 ELETTRONICO 43 crossref 2-s2.0-85103236349 http://hdl.handle.net/11365/1136100 https://complete.bioone.org/journals/waterbirds/volume-43/issue-2/063.043.0209/Reliability-of-Molecular-Sex-Identification-in-the-Adélie-Penguin-Pygoscelis/10.1675/063.043.0209.short doi:10.1675/063.043.0209 Mori, E., Brunetti, C., Carapelli, A., & Olmastroni, S. (2020). Reliability of Molecular Sex Identification in the Adélie Penguin (Pygoscelis adeliae) from DNA-Poor Samples. WATERBIRDS, 43(2) [10.1675/063.043.0209]. |
op_doi |
https://doi.org/10.1675/063.043.0209 |
container_title |
Waterbirds |
container_volume |
43 |
container_issue |
2 |
_version_ |
1766174832425172992 |