Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla
In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[a]pyrene ([BaP], at 0, 0.1, 1, 10, and 50 mg/kg), P-naphthoflavone ([...
Published in: | Environmental Toxicology and Chemistry |
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Online Access: | http://hdl.handle.net/11365/11231 https://doi.org/10.1897/02-378 |
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ftunivsiena:oai:usiena-air.unisi.it:11365/11231 2024-04-14T08:01:01+00:00 Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla REGOLI, F. WINSTON, G. W. GORBI, S. FRENZILLI, G. NIGRO, M. CORSI, I. FOCARDI, S. Regoli, F. Winston, G. W. Gorbi, S. Frenzilli, G. Nigro, M. Corsi, I. Focardi, S. 2003 STAMPA http://hdl.handle.net/11365/11231 https://doi.org/10.1897/02-378 eng eng info:eu-repo/semantics/altIdentifier/pmid/12959540 info:eu-repo/semantics/altIdentifier/wos/WOS:000185639400023 volume:22 issue:9 firstpage:2120 lastpage:2129 numberofpages:10 journal:ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY http://hdl.handle.net/11365/11231 doi:10.1897/02-378 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242319037 info:eu-repo/semantics/closedAccess oxyradical oxidative stre DNA damage total oxyradical scavenging capacity cytochrome P450 info:eu-repo/semantics/article 2003 ftunivsiena https://doi.org/10.1897/02-378 2024-03-21T16:12:31Z In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[a]pyrene ([BaP], at 0, 0.1, 1, 10, and 50 mg/kg), P-naphthoflavone ([BNF], at 0, 0.1, 1, 10, and 50 mg/kg), Arochlor(R) 1254 (at 0, 0.1, 1, 10, and 50 mg/kg), and 2,3,7,8-tetrachlorodibenzo p-dioxin ([TCDD]; at 0, 0.01, 0.1, 1, and 2 mug/kg). The integral relationships were analyzed between induction of ethoxyresorufin O-deethylase (EROD) activity, its involvement in perturbing oxyradical metabolism, and the role of cytochrome P450 and/or oxidative stress in mediating genotoxic effects. To reveal whether the oxidative status in exposed organisms was altered as a result of chemical exposure, measurements of the main endogenous antioxidant defenses were integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals (-OH). This approach permits discriminating the resistance of a tissue toward different forms of oxyradicals, thereby indicating a differential role for specific reactive oxygen species (ROS) in perturbing the balance between prooxidant and antioxidant mechanisms. All the analyzed chemicals promoted EROD induction (reflective of CYP1A) and altered either the levels or the activities of the antioxidants studied, which might be anticipated to exert alterations in oxyradical metabolism. Analysis of TOSC suggested the prevalence of metabolic oxidative pathways leading to the more reactive -OH on exposure to the chemicals studied. Of these chemicals, enhanced EROD activity correlated with genotoxic damage only in the cases of the nonhalogenated hydrocarbons BaP and BNF. The highest degree of genotoxic damage was consistently observed in organisms in which the capacity to absorb or scavenge -OH was lowest. These data suggest a general relationship between oxidative stress and loss of DNA integrity in juvenile eels exposed ... Article in Journal/Newspaper Anguilla anguilla European eel Università degli Studi di Siena: USiena air Environmental Toxicology and Chemistry 22 9 2120 |
institution |
Open Polar |
collection |
Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
oxyradical oxidative stre DNA damage total oxyradical scavenging capacity cytochrome P450 |
spellingShingle |
oxyradical oxidative stre DNA damage total oxyradical scavenging capacity cytochrome P450 REGOLI, F. WINSTON, G. W. GORBI, S. FRENZILLI, G. NIGRO, M. CORSI, I. FOCARDI, S. Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla |
topic_facet |
oxyradical oxidative stre DNA damage total oxyradical scavenging capacity cytochrome P450 |
description |
In this work, susceptibility to oxidative stress was analyzed under laboratory conditions in the European eel Anguilla anguilla. Eels were treated with increasing concentrations of benchmark environmental pollutants, namely, benzo[a]pyrene ([BaP], at 0, 0.1, 1, 10, and 50 mg/kg), P-naphthoflavone ([BNF], at 0, 0.1, 1, 10, and 50 mg/kg), Arochlor(R) 1254 (at 0, 0.1, 1, 10, and 50 mg/kg), and 2,3,7,8-tetrachlorodibenzo p-dioxin ([TCDD]; at 0, 0.01, 0.1, 1, and 2 mug/kg). The integral relationships were analyzed between induction of ethoxyresorufin O-deethylase (EROD) activity, its involvement in perturbing oxyradical metabolism, and the role of cytochrome P450 and/or oxidative stress in mediating genotoxic effects. To reveal whether the oxidative status in exposed organisms was altered as a result of chemical exposure, measurements of the main endogenous antioxidant defenses were integrated with the measurement of total oxyradical scavenging capacity (TOSC) toward peroxyl radicals and hydroxyl radicals (-OH). This approach permits discriminating the resistance of a tissue toward different forms of oxyradicals, thereby indicating a differential role for specific reactive oxygen species (ROS) in perturbing the balance between prooxidant and antioxidant mechanisms. All the analyzed chemicals promoted EROD induction (reflective of CYP1A) and altered either the levels or the activities of the antioxidants studied, which might be anticipated to exert alterations in oxyradical metabolism. Analysis of TOSC suggested the prevalence of metabolic oxidative pathways leading to the more reactive -OH on exposure to the chemicals studied. Of these chemicals, enhanced EROD activity correlated with genotoxic damage only in the cases of the nonhalogenated hydrocarbons BaP and BNF. The highest degree of genotoxic damage was consistently observed in organisms in which the capacity to absorb or scavenge -OH was lowest. These data suggest a general relationship between oxidative stress and loss of DNA integrity in juvenile eels exposed ... |
author2 |
Regoli, F. Winston, G. W. Gorbi, S. Frenzilli, G. Nigro, M. Corsi, I. Focardi, S. |
format |
Article in Journal/Newspaper |
author |
REGOLI, F. WINSTON, G. W. GORBI, S. FRENZILLI, G. NIGRO, M. CORSI, I. FOCARDI, S. |
author_facet |
REGOLI, F. WINSTON, G. W. GORBI, S. FRENZILLI, G. NIGRO, M. CORSI, I. FOCARDI, S. |
author_sort |
REGOLI, F. |
title |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla |
title_short |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla |
title_full |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla |
title_fullStr |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla |
title_full_unstemmed |
Integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and DNA damage in the European eel Anguilla anguilla |
title_sort |
integrating enzymatic responses to organic chemical exposure with total oxyradical absorbing capacity and dna damage in the european eel anguilla anguilla |
publishDate |
2003 |
url |
http://hdl.handle.net/11365/11231 https://doi.org/10.1897/02-378 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/12959540 info:eu-repo/semantics/altIdentifier/wos/WOS:000185639400023 volume:22 issue:9 firstpage:2120 lastpage:2129 numberofpages:10 journal:ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY http://hdl.handle.net/11365/11231 doi:10.1897/02-378 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-0242319037 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1897/02-378 |
container_title |
Environmental Toxicology and Chemistry |
container_volume |
22 |
container_issue |
9 |
container_start_page |
2120 |
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1796306280691793920 |