Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758)
The aim of this study is to investigate the effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver phase I and II biotransformation enzymes at gene (CYP1A1, GST and UDPGT) and catalytic levels by investigating EROD, GST and UDPGT activities, on crucial brain steroidogenic proteins (StAR and P...
Published in: | WIT Transactions on Ecology and the Environment, Environmental Toxicology II |
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ftunivsiena:oai:usiena-air.unisi.it:11365/1094935 2024-04-14T08:01:00+00:00 Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) Della Torre C. Corsi I. Sensini C. Arukwe A. Focardi S. Kungolos, A Zamorano, M Della Torre, C. Corsi, I. Sensini, C. Arukwe, A. Focardi, S. 2008 STAMPA http://hdl.handle.net/11365/1094935 https://doi.org/10.2495/ETOX080321 http://library.witpress.com/pages/listBooks.asp?tID=4 eng eng WITPress country:GBR place:ASHURST LODGE, SOUTHAMPTON SO40 7AA, ASHURST, ENGLAND info:eu-repo/semantics/altIdentifier/isbn/9781845641146 info:eu-repo/semantics/altIdentifier/wos/WOS:000257704900032 ispartofbook:ENVIRONMENTAL TOXICOLOGY II volume:110 firstpage:305 lastpage:314 numberofpages:10 serie:WIT TRANSACTIONS ON ECOLOGY AND THE ENVIRONMENT http://hdl.handle.net/11365/1094935 doi:10.2495/ETOX080321 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-58849146639 http://library.witpress.com/pages/listBooks.asp?tID=4 2,4,6-trinitrotoluene EROD European eel Gill histology GST Neurosteroidogenesi P450scc StAR UDPGT info:eu-repo/semantics/bookPart 2008 ftunivsiena https://doi.org/10.2495/ETOX080321 2024-03-21T16:07:18Z The aim of this study is to investigate the effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver phase I and II biotransformation enzymes at gene (CYP1A1, GST and UDPGT) and catalytic levels by investigating EROD, GST and UDPGT activities, on crucial brain steroidogenic proteins (StAR and P450scc genes expression) and on gills histology in a model fish species such as the European eel (Anguilla anguilla, Linnaeus, 1758). Eels were exposed in vivo for 6 and 24 hours to 0.5mg/L, 1mg/L and 2.5mg/L nominal concentration of TNT by using 0.1 of DMSO as a carrier. The TNT produced a significant inhibition of EROD activity and an increase of UDPG T and GST genes expression and activities compared to controls. A decrease of StAR and P450scc genes was also observed in TNT exposed eels. Finally concerning gills, branchial lifting was evident at the lowest TNT concentration (0.5 mg/L) while lamellar aneurisms, vascular congestion, hypertrophy and hyperplasia of the epithelium were evident at 2.5 mg/L. Our results highlighted the concern related to the release of TNT on the seabed: the inhibition of EROD activity may result in an increased susceptibility of the organism to P450 inducers such as dioxins and PAH. TNT also seems to affect fish neurosteroidogenesis by downregulating key enzymes (StAR and P450scc genes). Gills seem to be a target organ for TNT in fish. The present research provided relevant information on TNT metabolism/toxicity and indicated sensitive targets of TNT toxicity in fish species. © 2008 WIT Press. Book Part Anguilla anguilla European eel Università degli Studi di Siena: USiena air WIT Transactions on Ecology and the Environment, Environmental Toxicology II 1 305 314 |
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Università degli Studi di Siena: USiena air |
op_collection_id |
ftunivsiena |
language |
English |
topic |
2,4,6-trinitrotoluene EROD European eel Gill histology GST Neurosteroidogenesi P450scc StAR UDPGT |
spellingShingle |
2,4,6-trinitrotoluene EROD European eel Gill histology GST Neurosteroidogenesi P450scc StAR UDPGT Della Torre C. Corsi I. Sensini C. Arukwe A. Focardi S. Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) |
topic_facet |
2,4,6-trinitrotoluene EROD European eel Gill histology GST Neurosteroidogenesi P450scc StAR UDPGT |
description |
The aim of this study is to investigate the effects of the explosive 2,4,6-trinitrotoluene (TNT) on liver phase I and II biotransformation enzymes at gene (CYP1A1, GST and UDPGT) and catalytic levels by investigating EROD, GST and UDPGT activities, on crucial brain steroidogenic proteins (StAR and P450scc genes expression) and on gills histology in a model fish species such as the European eel (Anguilla anguilla, Linnaeus, 1758). Eels were exposed in vivo for 6 and 24 hours to 0.5mg/L, 1mg/L and 2.5mg/L nominal concentration of TNT by using 0.1 of DMSO as a carrier. The TNT produced a significant inhibition of EROD activity and an increase of UDPG T and GST genes expression and activities compared to controls. A decrease of StAR and P450scc genes was also observed in TNT exposed eels. Finally concerning gills, branchial lifting was evident at the lowest TNT concentration (0.5 mg/L) while lamellar aneurisms, vascular congestion, hypertrophy and hyperplasia of the epithelium were evident at 2.5 mg/L. Our results highlighted the concern related to the release of TNT on the seabed: the inhibition of EROD activity may result in an increased susceptibility of the organism to P450 inducers such as dioxins and PAH. TNT also seems to affect fish neurosteroidogenesis by downregulating key enzymes (StAR and P450scc genes). Gills seem to be a target organ for TNT in fish. The present research provided relevant information on TNT metabolism/toxicity and indicated sensitive targets of TNT toxicity in fish species. © 2008 WIT Press. |
author2 |
Kungolos, A Zamorano, M Della Torre, C. Corsi, I. Sensini, C. Arukwe, A. Focardi, S. |
format |
Book Part |
author |
Della Torre C. Corsi I. Sensini C. Arukwe A. Focardi S. |
author_facet |
Della Torre C. Corsi I. Sensini C. Arukwe A. Focardi S. |
author_sort |
Della Torre C. |
title |
Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) |
title_short |
Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) |
title_full |
Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) |
title_fullStr |
Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) |
title_full_unstemmed |
Acute sublethal effects of 2,4,6-trinitrotoluene (TNT) on the European eel Anguilla anguilla (Linnaeus, 1758) |
title_sort |
acute sublethal effects of 2,4,6-trinitrotoluene (tnt) on the european eel anguilla anguilla (linnaeus, 1758) |
publisher |
WITPress |
publishDate |
2008 |
url |
http://hdl.handle.net/11365/1094935 https://doi.org/10.2495/ETOX080321 http://library.witpress.com/pages/listBooks.asp?tID=4 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
info:eu-repo/semantics/altIdentifier/isbn/9781845641146 info:eu-repo/semantics/altIdentifier/wos/WOS:000257704900032 ispartofbook:ENVIRONMENTAL TOXICOLOGY II volume:110 firstpage:305 lastpage:314 numberofpages:10 serie:WIT TRANSACTIONS ON ECOLOGY AND THE ENVIRONMENT http://hdl.handle.net/11365/1094935 doi:10.2495/ETOX080321 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-58849146639 http://library.witpress.com/pages/listBooks.asp?tID=4 |
op_doi |
https://doi.org/10.2495/ETOX080321 |
container_title |
WIT Transactions on Ecology and the Environment, Environmental Toxicology II |
container_volume |
1 |
container_start_page |
305 |
op_container_end_page |
314 |
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1796306121226452992 |