Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.)
Lower jaw deformity (LJD) is a skeletal anomaly affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads to considerable economic losses for industry and has animal welfare implications. The present study employed transcriptome analysis in parallel with real-time qPCR techniques to cha...
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ftunivscoast:usc:21267 2023-05-15T15:31:05+02:00 Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) Amoroso, Gianluca Ventura, T Cobcroft, J M Adams, Mark B Elizur, A Carter, Chris G 2016 https://doi.org/10.1371/journal.pone.0168454 eng eng Public Library of Science usc:21267 URN:ISSN: 1932-6203 Copyright: © 2016 Amoroso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY FoR multidisciplinary Journal Article 2016 ftunivscoast https://doi.org/10.1371/journal.pone.0168454 2020-04-20T22:26:27Z Lower jaw deformity (LJD) is a skeletal anomaly affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads to considerable economic losses for industry and has animal welfare implications. The present study employed transcriptome analysis in parallel with real-time qPCR techniques to characterise for the first time the LJD condition in triploid Atlantic salmon juveniles using two independent sample sets: experimentally-sourced salmon (60 g) and commercially produced salmon (100 g). A total of eleven genes, some detected/identified through the transcriptome analysis (fbn2, gal and gphb5) and others previously determined to be related to skeletal physiology (alp, bmp4, col1a1, col2a1, fgf23, igf1, mmp13, ocn), were tested in the two independent sample sets. Gphb5, a recently discovered hormone, was significantly (P < 0.05) down-regulated in LJD affected fish in both sample sets, suggesting a possible hormonal involvement. In-situ hybridization detected gphb5 expression in oral epithelium, teeth and skin of the lower jaw. Col2a1 showed the same consistent significant (P < 0.05) down-regulation in LJD suggesting a possible cartilaginous impairment as a distinctive feature of the condition. Significant (P < 0.05) differential expression of other genes found in either one or the other sample set highlighted the possible effect of stage of development or condition progression on transcription and showed that anomalous bone development, likely driven by cartilage impairment, is more evident at larger fish sizes. The present study improved our understanding of LJD suggesting that a cartilage impairment likely underlies the condition and col2a1 may be a marker. In addition, the involvement of gphb5 urges further investigation of a hormonal role in LJD and skeletal physiology in general. Article in Journal/Newspaper Atlantic salmon Salmo salar University of the Sunshine Coast, Queensland, Australia: COAST Research Database PLOS ONE 11 12 e0168454 |
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University of the Sunshine Coast, Queensland, Australia: COAST Research Database |
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language |
English |
topic |
FoR multidisciplinary |
spellingShingle |
FoR multidisciplinary Amoroso, Gianluca Ventura, T Cobcroft, J M Adams, Mark B Elizur, A Carter, Chris G Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) |
topic_facet |
FoR multidisciplinary |
description |
Lower jaw deformity (LJD) is a skeletal anomaly affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads to considerable economic losses for industry and has animal welfare implications. The present study employed transcriptome analysis in parallel with real-time qPCR techniques to characterise for the first time the LJD condition in triploid Atlantic salmon juveniles using two independent sample sets: experimentally-sourced salmon (60 g) and commercially produced salmon (100 g). A total of eleven genes, some detected/identified through the transcriptome analysis (fbn2, gal and gphb5) and others previously determined to be related to skeletal physiology (alp, bmp4, col1a1, col2a1, fgf23, igf1, mmp13, ocn), were tested in the two independent sample sets. Gphb5, a recently discovered hormone, was significantly (P < 0.05) down-regulated in LJD affected fish in both sample sets, suggesting a possible hormonal involvement. In-situ hybridization detected gphb5 expression in oral epithelium, teeth and skin of the lower jaw. Col2a1 showed the same consistent significant (P < 0.05) down-regulation in LJD suggesting a possible cartilaginous impairment as a distinctive feature of the condition. Significant (P < 0.05) differential expression of other genes found in either one or the other sample set highlighted the possible effect of stage of development or condition progression on transcription and showed that anomalous bone development, likely driven by cartilage impairment, is more evident at larger fish sizes. The present study improved our understanding of LJD suggesting that a cartilage impairment likely underlies the condition and col2a1 may be a marker. In addition, the involvement of gphb5 urges further investigation of a hormonal role in LJD and skeletal physiology in general. |
format |
Article in Journal/Newspaper |
author |
Amoroso, Gianluca Ventura, T Cobcroft, J M Adams, Mark B Elizur, A Carter, Chris G |
author_facet |
Amoroso, Gianluca Ventura, T Cobcroft, J M Adams, Mark B Elizur, A Carter, Chris G |
author_sort |
Amoroso, Gianluca |
title |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) |
title_short |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) |
title_full |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) |
title_fullStr |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) |
title_full_unstemmed |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.) |
title_sort |
multigenic delineation of lower jaw deformity in triploid atlantic salmon (salmo salar l.) |
publisher |
Public Library of Science |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0168454 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
usc:21267 URN:ISSN: 1932-6203 |
op_rights |
Copyright: © 2016 Amoroso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0168454 |
container_title |
PLOS ONE |
container_volume |
11 |
container_issue |
12 |
container_start_page |
e0168454 |
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1766361583384002560 |